In this article, we provide an overview of the liquid chromatography process and instrument components, as well as the differences between HPLC and UHPLC analytical techniques.
Liquid chromatography (LC) supports a wide range of applications and is ideal for laboratories with demands to increase throughput, analytical flexibility and performance. High performance liquid chromatography (HPLC) has traditionally been viewed as one of the most commonly utilized analytical techniques, but as the need for faster separations, better peak resolution and lower detection limits has increased in recent years, many labs have begun to transition their HPLC methods to ultra high-performance liquid chromatography (UHPLC) techniques.
In this series of articles, we will explore many of the aspects of HPLC to UHPLC method conversion, providing practical and actionable information to help your laboratory determine if converting to UHPLC is the right choice, as well as how to effectively transition a method while maintaining data quality and consistency.
Transferring methods from HPLC to UHPLC can result in efficiency gains and improved data quality. In this article, the benefits of UHPLC are discussed, as well as a number of points to consider before transferring a method.
Best Practices in UHPLC Method Transfer
There are a number of best practices that should be followed when transferring a method from HPLC to UHPLC. This article presents recommendations on choosing the right column and calculating method parameters to ensure a smooth transition to UHPLC.
Case Study in UHPLC Method Transfer
Following on our Best Practices in UHPLC Method Transfer article, this case study applies method transfer calculations to a nutraceutical application to demonstrate efficiency gains utilizing UHPLC.