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Determination of Mycotoxins in Cereals by LC/MS/MS with Online SPE

Mycotoxins are toxic secondary metabolites produced by fungi, and are capable of causing disease and death in both humans and animals. Sample preparation is a critical step in the successful analysis of mycotoxins in food matrices. The "dilute and shoot" approach is a quick and easy way to introduce the sample into the LC/MS/MS analysis. However, due to the complexity of food matrices, this approach will often result in irreproducible matrix effects. To overcome these problems and improve sensitivity, this application note details the use of online solid phase extraction (SPE), coupled to an LC/MS/MS system for sample enrichment.

Starches (cationic) method - RVA ST04.03

The method is applicable to cationic corn starches (waxy and dent), including those that have also been acidthinned. These industrial starches are used widely for wet end, size press and coating applications in papermaking. High shear is used during cooking to approximate typical processing conditions for thesestarches.

Starches (acid thinned) method - RVA ST05.03

The method is applicable to unmodified and to acid thinned dent corn starches. These food and industrial starches are used as thickeners, gelling agents, sizes and coatings in products such as puddings, gravies, sauces, confection and paper. The final temperature of 65°C (149°F) is used to rapidly stabilize viscosity and minimize retrogradation. The Rapid Visco Analyser (RVA) is a cooking stirring viscometer with ramped temperature and variable shear profiles optimized for testing viscous properties.

Starches (dent cook-up) method - RVA ST02.03

This method is applicable to substituted (acetyl or hydroxypropyl), crosslinked, and both substituted and crosslinked dent corn starch. These cook-up food starches are used as thickeners, stabilizers and clarifiers in products such as soups, puddings, pie fillings and retorted foods. An extended hold time at 95°C (203°F) is used to assist in swelling the crosslinked starches and to better differentiate these products.

High Temperature Jet Cooking Method - RVA 48.01

This high temperature RVA method is applicable to any unmodifies and modified starches and their derivatives, including composites with hydrocolloids and lipids. High shear is used in the method to approximate typical processing conditions for these ingredients, which are commonly used as far replacers, gelling agents and stabilizers, and as paper wet end, size press and coating additives.

High Temperature General Pasting Method - RVA 45.01

The ability of the components of a product to withstand high-temperature processing conditions is of interest to formulators, who must balance the need for acceptable product rheology with safety and throughput. The Rapid Visco Analyser (RVA) can perform high temperature tests. The method presented in this document is applicable to any liquid or hydrated ground material including, but not limited to, unmodified starches, flours, whole meals, gums, diary ingredients, and other formulations.

Starches (dent cook-up) method - RVA ST02.03

This method is applicable to substituted (acetyl or hydroxypropyl), crosslinked, and both substituted and crosslinked dent corn starch. These cook-up food starches are used as thickeners, stabilizers and clarifiers in products such as soups, puddings, pie fillings and retorted foods. An extended hold time at 95°C (203°F) is used to assist in swelling the crosslinked starches and to better differentiate these products.

High Temperature Retorting Method - RVA 46.01

This high-temperature RVA method is designed to mimimc retorting, allowing assessment of the suitabilitu and stability of polysaccharide thickeners (eg. Gums, modified starches) in the formulation for high temperature processing.

Corn Starch Method

This method is applicable to unmodified corn and other cereal starches. A 13-minute test in the RVA provides a rapid yet accurate alternative to study the cooking properties of unmodified cereal starches, making it suitable for both research and quality control applications.

Grain Testing Solutions Brochure

The grain industry is very complex. It’s global, diverse, and can also present analytical challenges. Today’s grain users demand more when it comes to quality, safety, and uniformity. In addition, they seek diverse products with unique characteristics. PerkinElmer is equipped to help the grain industry in its quest to feed the world – nutritiously and economically. Our testing and analysis solutions encompass the three primary areas required for complete knowledge of grains and their derivatives – composition, functionality, and safety.

Determination of Mycotoxins in Cereals by LC/MS/MS with Online SPE

Mycotoxins are toxic secondary metabolites produced by fungi, and are capable of causing disease and death in both humans and animals. Sample preparation is a critical step in the successful analysis of mycotoxins in food matrices. The "dilute and shoot" approach is a quick and easy way to introduce the sample into the LC/MS/MS analysis. However, due to the complexity of food matrices, this approach will often result in irreproducible matrix effects. To overcome these problems and improve sensitivity, this application note details the use of online solid phase extraction (SPE), coupled to an LC/MS/MS system for sample enrichment.

Malt flour method - RVA 03.04

The Rapid Visco Analyser (RVA) is a cooking stirring viscometer with rampedtemperature and variable shear profiles optimized for testing viscous properties.The instrument includes international standard methods as well as full flexibilityfor customer tailor-made profiles. Combining speed, precision, flexibility andautomation, the RVA is a unique tool for product development, quality andprocess control and quality assurance.

Starches (cationic) method - RVA ST04.03

The method is applicable to cationic corn starches (waxy and dent), including those that have also been acidthinned. These industrial starches are used widely for wet end, size press and coating applications in papermaking. High shear is used during cooking to approximate typical processing conditions for thesestarches.

Starches (acid thinned) method - RVA ST05.03

The method is applicable to unmodified and to acid thinned dent corn starches. These food and industrial starches are used as thickeners, gelling agents, sizes and coatings in products such as puddings, gravies, sauces, confection and paper. The final temperature of 65°C (149°F) is used to rapidly stabilize viscosity and minimize retrogradation. The Rapid Visco Analyser (RVA) is a cooking stirring viscometer with ramped temperature and variable shear profiles optimized for testing viscous properties.

Starches (dent cook-up) method - RVA ST02.03

This method is applicable to substituted (acetyl or hydroxypropyl), crosslinked, and both substituted and crosslinked dent corn starch. These cook-up food starches are used as thickeners, stabilizers and clarifiers in products such as soups, puddings, pie fillings and retorted foods. An extended hold time at 95°C (203°F) is used to assist in swelling the crosslinked starches and to better differentiate these products.

General pasting (91 'C) method - RVA 44.01

The method uses a maximum test temperature of 91ºC, to avoid boiling of water in the RVA canister at high altitudes (900-2100 m, or 3000-7000 ft). The pasting properties of starch and starch-containing products are readily assessed in the RVA. During the test, the starch is gelatinized with consequent rise in viscosity, subject to high temperature and controlled shear during which its stability is revealed, then cooled to provide an indication of setback during gelation. Samples can be assessed for pasting temperature, peak paste viscosity, time to peak, temperature at peak, hot and cold paste viscosity, breakdown, setback, final viscosity and other parameters.

Corn Starch Method

This method is applicable to unmodified corn and other cereal starches. A 13-minute test in the RVA provides a rapid yet accurate alternative to study the cooking properties of unmodified cereal starches, making it suitable for both research and quality control applications.

Grain Testing Solutions Brochure

The grain industry is very complex. It’s global, diverse, and can also present analytical challenges. Today’s grain users demand more when it comes to quality, safety, and uniformity. In addition, they seek diverse products with unique characteristics. PerkinElmer is equipped to help the grain industry in its quest to feed the world – nutritiously and economically. Our testing and analysis solutions encompass the three primary areas required for complete knowledge of grains and their derivatives – composition, functionality, and safety.