HPLC & UHPLC Instruments

Liquid Chromatography

Liquid chromatography is a vitally important technology utilized across a number of industries, including food safety, cannabis/hemp production, pharmaceutical development, CMC, manufacturing and QA/QC, environmental monitoring and various industrial and consumer product applications. Although these industries may vary greatly, the LC applications they utilize all demand precision, accuracy and flexibility to meet their unique analytical goals.

Whether your application requires the ruggedness of high-performance liquid chromatography (HPLC), the precision and power of ultra-high performance chromatography (UHPLC), or the flexibility of an HPLC-ICP-MS speciation solution, PerkinElmer offers the right technology to meet your analytical goals. Our analytical solutions, including a full suite of chromatography data system (CDS) software packages, consumables and service offerings, come together to deliver a total liquid chromatography solution to increase your throughput, reduce costs and limit unnecessary complexity in analytical processes.


LC 300 UHPLC System

Our LC 300 UHPLC is the system of choice for applications that demand superior performance and sensitivity. With ultra-precise gradient flows up to 18,000 psi/1,240 bar, the LC 300 UHPLC system delivers the power and increased throughput required for even the most demanding applications.

Key features of the LC 300 UHPLC platform include:

  • 18,000 psi (1240 bar) autosampler and pump configuration.
  • Patented ILD injection valve design reduces pressure and shock to improve data quality and increase column lifetimes.
  • Integrated solvent containment and column oven for reduced system complexity
  • Wide range of detectors: PDA, MWD, UV/Vis, FL and RI
  • Automated sample dilution and derivatization
  • Full instrument control and data processing with the SimplicityChrom CDS software

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LC 300 HPLC System

The next generation LC 300 HPLC system delivers the accuracy and flexibility needed for routine liquid chromatography applications. With its robust feature set and intuitive operation, the LC 300 HPLC has been designed to increase your laboratory productivity and throughput, even in the face of challenging budget and staffing situations.

Key features of the LC 300 HPLC platform include:

  • 10,000 psi (690 bar) autosampler and pump configuration.
  • High visibility color display for immediate assessment of system status without having to log into your CDS software.
  • Outstanding injection reproducibility – even at low volumes
  • Three injection modes: full loop, partial fill and µL-pickup for maximum flexibility
  • Long seal life via a dedicated and automated piston seal wash mechanism
  • Wide range of detectors: PDA, MWD, UV/Vis, FL and RI

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NexSAR Speciation Analysis Ready HPLC System

Next-generation Speciation Analysis Ready system engineered with a completely inert and metal-free fluid path, enabling laboratories to meet low chromatographic background requirements on the most challenging speciation applications.

  • Inert HPLC pump engineered to deliver pulse-free and quiet baselines, with a fluid path that is completely metal-free and composed of inert materials.
  • Inert/cooled inert HPLC autosampler with metal-free and inert fluid path available in either cooled or non-cooled configurations
  • Ergonomically designed solvent tray reduces cavitation and stores solvent bottles in an inert holder, preventing leaks or spillages from damaging the rest of the system.
  • Optional three-channel degasser and solvent tray allows degassing of up to three different solvents, minimizing the risk of air bubbles from entering the system, which would otherwise affect pump and column performance.

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SimplicityChrom CDS Software

Laboratories in nearly every industry are continuously faced with growing demands for increased throughput, improved margins, and more efficient processes. To meet these goals, you need a solution that creates an effective, secure, and easy-to-use workflow environment.

The SimplicityChrom is a modern, intuitive and flexible CDS software platform that enables you to efficiently control the instrument, process data, and generate results. Pair this functionality with the peace of mind of 21 CFR Part 11 compliance and industry-leading service and support, and you're free to focus on what matters most - your analytical goals.

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LC 300 Resources

Analysis of Common Preservatives in Personal Care Products by HPLC with UV Detection

Parabens are utilized as an antimicrobial preservative in many commercial offerings including cosmetics, personal care products and foods, and offer both chemical and thermal stability to products. Although parabens increase the safety of products, recent studies have suggested that parabens may exh ...

Analysis of Phytoestrogen Isoflavones in Dietary Supplements by HPLC/UV

Isoflavones are water-soluble compounds found in many plant and food sources. Designated as phytoestrogens, isoflavones are structurally similar to to the female hormone estrogen, and have been shown to exhibit a number of beneficial impacts to human health, such as a reduction in the development of ...

Analysis of Sugars in Maple Syrup by HILIC with Refractive Index Detection

Maple syrup, a common natural sweetener, is consumed regularly by households around the world. Quantification of sugar content in maple syrup is paramount to satisfy product labeling requirements and ensure label-claim accuracy. Although sucrose is the primary sugar component in maple syrup, a compl ...

Analysis of 16 Cannabinoids Using the PerkinElmer LC 300 HPLC System with PDA Detection

Accurate identification and quantification of cannabinoid content is a critical step in the process to bringing safe and high quality cannabis flower and fortified products to market. As the legalization of recreational cannabis use continues throughout the world, fast and efficient liquid chromatog ...

PerkinElmer LC 300 Autosampler Intermediate Loop Decompression (ILD) Injection Valve

The injection of an accurate and reproducible sample volume into a UHPLC system is challenging, but essential to ensuring a high quality analysis. The patented PerkinElmer Intermediate Loop Decompression (ILD) valve, featured in the LC 300 Autosampler, is a 7-port valve with a strategically placed r ...

HPLC Columns: The Foundation of Great Chromatography

The foundation of great Chromatography starts with selecting the right column. Our LC column portfolio brings you new opportunities for full performance-boosting LC workflow solutions, ensuring that you have the optimum column for your application.

LC Applications

Analysis of Drug Substances in Common Cold Medicines with the PerkinElmer Flexar FX-15 System Equipp

The misuse of cold remedies can be prevented by appropriate medical indications and accurate label claims. To that end, the U.S. Food and Drug Administration and the pharmaceutical industry have made it a standard procedure to routinely test drug products to ensure the accuracy of the amount of acti ...

Analysis of Organic Acids in Fruit Juices by HPLC and UV Detection

Organic acids, used as preservatives, acidulants or stabilizers in food, contribute to the sensory properties of foods and beverages by providing both taste and aroma. Monitoring the content of organic acids in foods is an important step in the food manufacturing process, as well as quality and regu ...

PAHs in Surface Water by PDA and Fluorescence Detection

Polycyclic aromatic hydrocarbons (PAHs) are prevalent in a number of environmental matrices, and are created as a result of the combustion of fossil fuels. PAHs are water soluble and toxic to invertebrate aquatic organisms. They have also been shown to bioaccumulate in the environment, and their sol ...

The Analysis of Sunscreen Active Ingredients and Parabens in Lotions and Lip Balms by UHPLC with PDA Detection

Cosmetics utilized to prevent skin damage from the sun’s radiation often include ingredients which can pose a risk to human health at high enough concentrations. Oxybenzone, for example, can contribute to melanoma associated with the production of DNA-attacking free radicals upon UV exposure. Parabe ...

NexSAR Resources

Characterization of Arsenic Species in Apple Juice using a NexSAR HPLC-ICP-MS Speciation Analysis Ready Solution

Although apple juice is a healthy, and often preferred, alternative to artificially flavored and carbonated drinks for many parents to give to children, concern has been raised over the presence of arsenic within. This work demonstrates a reversed-phase ion-pairing method for the complete separation ...

Quantification of Low Levels of Hexavalent Chromium in Water Using a NexSAR HPLC-ICP-MS

Due to rising concerns over the carcinogenic properties of hexavalent chromium (Cr6+) in drinking water, many national and regional water standards are looking to lower the maximum allowable levels of total chromium and hexavalent chromium in potable waters. And in order to assess trace concentratio ...

Speciation of Hexavalent Chromium in Children's Toys According to EN 71-3 Category II with the NexSAR HPLC-ICP-MS

Testing for harmful metal(loid)s that may be included in raw materials, processes, paints and additives used in the manufacturing processes of children's toys has been mandated by the regulation EN 71-3. This regulation recommends specific testing methods and maximum allowable concentrations of leac ...

NexSAR HPLC-ICP-MS Speciation Solution

For labs looking to expand their analytical portfolio to include HPLC-ICP-MS, PerkinElmer's NexSAR HPLC-ICP-MS brings together the inert NexSAR speciation analysis ready HPLC system and the revolutionary NexION ICP-MS in a seamless platform, integrated using Clarity chromatography software.

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