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LC 300 UHPLC System

The PerkinElmer LC 300 platform redefines the liquid chromatography experience, offering the flexibility, performance and efficiency needed to tackle even the most challenging analytical demands. With the option to customize your experience with a wide range of detectors and accessories, each LC 300 system is designed to meet the most important specifications – yours. Paired with SimplicityChrom CDS software, the LC 300 UHPLC system delivers an elevated user experience with simplified liquid chromatography workflows, intuitive functions, and automated processes.

Part Number N2550120
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The PerkinElmer LC 300 UHPLC system has been designed to increase laboratory productivity and throughput, even in the face of challenging budget and staffing situations. Paired with the highly intuitive and powerful SimplicityChrom chromatography data system (CDS) software, our LC 300 systems are the ideal solution for labs seeking to maximize their liquid chromatography experience.

The LC 300 offers superior performance and flexibility throughout its intelligent design:

  • Robust and precise autosampler with integrated column oven and preheater, as well as a high visibility color LCD display showing full instrument system status, current queue and sample progress.
  • Optional external Peltier column oven for ultra-precise heating and cooling, for sample integrity and exceptionally accurate results.
  • Patented Intermediate Loop Decompression (ILD™) valve ensures the injection of an accurate and reproducible sample volume, while also increasing the lifetime of columns used in analysis.
  • Automated, onboard sample dilution and derivatization capabilities simplify the front-end analytical processes, while also ensuring reproducibility and precision.
  • Powerful 18k psi pump for ultra-precise UHPLC analyses, delivering repeatable solvent delivery at a wide range of flow rates and pressures.
  • Automatic priming design allows the system to achieve full self-priming capabilities, automating the otherwise cumbersome priming process utilized in many other systems.
  • Built-in solvent degassing technology simplifies the analytical process, and obviates the need for a separate, external degassing module.
  • Portfolio of five available detectors, which can be used individually or in sequence.
    • Photodiode array (PDA)
    • Multiwavelength UV/Vis (MWD)
    • Single wavelength UV/Vis
    • Refractive index (RI)
    • Fluorescence (FL)
  • Efficient and powerful instrument control with SimplicityChrom CDS, allowing users to create custom workflows and experiences, all while achieving compliance with 21 CFR Part 11 requirements.


Depth 22 inches
Height 38.2 inches maximum (single stack with detector and standalone solvent organizer)
Weight 183 pounds (single stack with one detector and standalone solvent organizer) or 260 pounds maximum (dual stack with external oven)
Width 13.5 inches maximum per component
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Application Brief

HPLC Analysis of Acetaminophen, Aspirin and Caffeine Using an Epic C18 Column in Accordance with the USP

Acetaminophen, aspirin and caffeine used in combination are an effective method of treating acute headaches and migraines. The combination of these substances is considered superior to acetaminophen on its own when treating headaches. There are other benefits due to the individual components of the tablets. Acetaminophen (also known as paracetamol) is the most common analgesic and antipyretic around the world, it is an effective treatment for acute primary headaches on its own and can be combined with tramadol to treat cluster headaches. It has a very low risk of causing allergic reactions and so can be used by those with bronchial asthma. Aspirin is a first-line therapy to treat moderate to severe primary headaches as well as reducing pain, fever and inflammation. However, it is not taken by children under twelve due to the risk of Reye syndrome. Caffeine is considered an effective treatment for various types of headaches including post-dural puncture headaches and hypnic headaches. It also provides an increase in high energy exercise tolerance and reduces fatigue. These drugs combined are commonly sold under the brand names Anadin Extra in the UK and Excedrin in the U.S. In 2019 approximately 13.1 million units of Excedrin Migraine were sold in the United States. This application brief describes the use of an ES Industries Epic C18 column for the analysis of acetaminophen, aspirin and caffeine in accordance with the official Acetaminophen, Aspirin and Caffeine Tablets United States Pharmacopeia (USP) method for the assay.

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HPLC Analysis of Amlodipine Besylate in Accordance with the USP

Amlodipine besylate belongs to a class of drugs known as dihydropyridine calcium channel blockers. It is primarily used to treat hypertension and angina as well as other conditions which arise as a result of coronary artery disease. As a calcium channel blocker, it prevents diffusion of calcium into cardiac muscle and the smooth muscle of the blood vessels. This causes vasodilation, lower heart contractility and therefore lower blood pressure. In addition, production of nitric oxide (a known vasodilator) increases. Amlodipine is listed on the World Health Organization’s (WHO) list of essential medicines as an antihypertensive medicine, meaning that it considered among the safest and most effective compounds needed to meet the requirements of a basic healthcare system. This application brief describes the use of our Epic C18 column to analyze amlodipine besylate in accordance with the official USP monograph.

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HPLC Analysis of Betamethasone Dipropionate Using Epic C18 HPLC Column

Glucocorticoid steroids work by suppressing various aspects of the human immune system in conditions where hyperactivity can cause poor health through allergies, inflammation, and autoimmune dysfunction. Betamethasone dipropionate belongs to this class of steroids. It is used for its high potency as an anti-inflammatory and immunosuppressant in the treatment of diseases such as eczema, dermatitis, and psoriasis. Betamethasone dipropionate is classified as a ‘super-potent’ steroid in the treatment of psoriasis in comparison with betamethasone valerate (another common analogue of betamethasone) which is rated as upper mid-strength. This application brief describes the use of an Epic C18 column for the analysis of betamethasone dipropionate in accordance with the official USP monograph.

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HPLC Analysis of Common Ginsenosides Found in Ginseng Using the Chinese Pharmacopeia

The term ginseng can refer to several natural products and herbal remedies usually produced from the plant, panax ginseng. Panax ginseng is considered a traditional Chinese medicine (TCM) as it has a reported wide range of medicinal properties such as antioxidant effects and promotion of immune function.1 The chemistry of ginseng is still not fully understood and more research is required to understand its functions as it becomes increasingly popular in western culture. The main active components of ginseng are ginsenosides of which there are over 100 in various forms of panax ginseng. Ginsenosides are named as Ginsenoside Rx, with x representing the movement on a thin layer chromatography plate, x represents a letter from a-h with a being the most polar and h being the least. This application brief describes the use of an Epic C18 to analyze three ginsenosides using a method based on the Chinese Pharmacopeia (ChP).

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HPLC Analysis of Finasteride Using an Epic C18 HPLC Column

Finasteride is an antiandrogenic compound or ‘testosterone blocker’ which is used as a treatment for an enlarged prostate and can treat male hair loss. It inhibits the production of the enzyme Type-II 5a-reductase which converts testosterone into the more active metabolite 5a-dihydrotestosterone (DHT). DHT is responsible for the development and enlargement of the prostate and can contribute to hair loss by shortening hair follicles and causing them to fall out more quickly. By suppressing production of DHT, finasteride directly targets the molecule responsible for stimulation of prostatic growth. Finasteride is commonly sold under the brand name Proscar and in 2016 was the 75th most prescribed drug in the USA. This application brief describes the use of an Epic C18 column to analyze finasteride in accordance with the official Finasteride Tablets USP monograph.

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Application Note

Analysis of 16 Cannabinoids Using the PerkinElmer LC 300 HPLC System with PDA Detection

Accurate identification and quantification of cannabinoid content is a critical step in the process to bringing safe and high quality cannabis flower and fortified products to market. As the legalization of recreational cannabis use continues throughout the world, fast and efficient liquid chromatography methods for the determination of cannabinoid content are need to satisfy both regulatory and consumer demands.

In this application note, a fast and simple method for the determination of 16 common cannabinoids is presented. Utilizing a PerkinElmer LC 300 HPLC system with a PDA detector, the method results in the elution of all sixteen compounds in under seven minutes, with LOQs = 0.15 µg/mL for most analytes.

Analysis of Common Preservatives in Personal Care Products by HPLC with UV Detection

Parabens are utilized as an antimicrobial preservative in many commercial offerings including cosmetics, personal care products and foods, and offer both chemical and thermal stability to products. Although parabens increase the safety of products, recent studies have suggested that parabens may exhibit weak endocrine disrupting effects on users of the products, an effect that can be further intensified by the ability of parabens to be absorbed by the skin without being broken done by esterase enzymes.

In this application note, a fast, robust and sensitive method utilizing a PerkinElmer LC 300 HPLC system with Multi-Wavelength UV/Vis (MWD) detector for the analysis of 2-phenoxyethanol and seven commonly used parabens in eight different commercially available cosmetic and personal care products is presented.

Analysis of Phytoestrogen Isoflavones in Dietary Supplements by HPLC/UV

Isoflavones are water-soluble compounds found in many plant and food sources. Designated as phytoestrogens, isoflavones are structurally similar to to the female hormone estrogen, and have been shown to exhibit a number of beneficial impacts to human health, such as a reduction in the development of certain cancers, as well as an improvement in cholesterol ratios. Studies also suggest, however, that the phytoestrogenic nature of isoflavones can also lead to an exacerbation of existing thyroid disorders, owing to a disruption to the endocrine system. As such, precise quantification of isoflavone content in nutraceutical products is paramount to ensure label-claim accuracy and consumer safety.

In this application note, an efficient and reliable method for the determination of six common isoflavone compounds is presented. The method, which was developed in accordance with the USP monograph for soy isoflavones in dietary supplement capsules, utilizes a PerkinElmer LC 300 UHPLC system, with subsequent detection of compounds achieved utilizing an LC 300 PDA detector.

Analysis of Sugars in Maple Syrup by HILIC with Refractive Index Detection

Maple syrup, a common natural sweetener, is consumed regularly by households around the world. Quantification of sugar content in maple syrup is paramount to satisfy product labeling requirements and ensure label-claim accuracy. Although sucrose is the primary sugar component in maple syrup, a complete identification and quantification of the other complex carbohydrates, including glucose, fructose and maltose, is essential.

In this application note, a robust and efficient method for the determination of the four aforementioned complex carbohydrates is presented. Utilizing an LC 300 HPLC system, a hydrophilic interaction chromatography (HILIC) technique is utilized with subsequent detection achieved utilizing an LC 300 Refractive Index (RI) detector.



Comprehensive Testing Solutions for Feed Millers

Feed milling today is a complex business, and agribusinesses both large and small need to balance the nutritional and safety needs of livestock with availability of raw ingredients and their seasonality and variability. Add to that the valuable supplementation and medication that millers supply, and it’s clear: you’re creating a complete nutritional delivery system, not just a simple mix of grains.

LC 300 HPLC and UHPLC Systems Interactive Brochure

Laboratories in nearly every industry are continuously faced with growing demands for increased throughput, improved margins, and more efficient processes. And when it comes to liquid chromatography, a one-size-fits-all approach just isn’t enough. You need an LC system tailored to your needs, with user-friendly software, consumables, service, and support – the total package.

Meet the PerkinElmer LC 300, the latest LC to step off our production lines. With features and benefits such as five available detectors, ultraprecise gradient flows, low dispersion, and new Simplicity Chrom CDS software, our LC 300 systems provide improved workflows, throughput, and usability.



Epic HPLC-UHPLC Column Phase Specifications Sheet

The Epic™ line is our latest range of LC columns, based on high density monomerically bonded phases produced through a proprietary bonding process. Epic HPLC and UHPLC columns are compatible with a wide range of organic modifiers and buffers and stable over a wide pH range. All Epic products use an ultra-high purity metal free silica and undergo strict quality control testing. For flexibility, we offer a wide range of column sizes as well being fully scalable from analytical to preparative dimensions. Whatever your separation need, we have a chemistry or dimension to fill it.

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SimplicityChrom CDS

SimplicityChrom is a modern, intuitive and flexible CDS software platform that enables users to efficiently control their instrument, process data, and generate results. Pair this functionality with the peace of mind of 21 CFR Part 11 compliance and industry-leading service and support, and you’re free to focus on what matters most – your analytical goals.


Product Note

LC 300 Product Note

The PerkinElmer LC 300 liquid chromatography systems are the result of listening to user needs, and paying careful attention to every detail in the resulting design. The LC 300 systems are the ideal solution for labs faced with high throughput requirements or challenging chromatographic separations.

Whatever the application, the PerkinElmer LC 300 system’s performance and enhanced user experience will ensure your lab’s success, whether that need be for pharmaceutical, food, consumer products or specialty chemicals.

In this product note, detailed information on each of the LC 300's modules is presented.

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Technical Note

Clone Columns-Harmony C18 and Waters Symmetry C18 Comparison

Legacy methods, by their very nature, often use older column technologies. These older phases can be accompanied by larger variations in batch-to-batch performance which can lead to inconsistent results and cause out of specification (OOS) occurrences. With routine analysis, often completed with compliant procedures, any unplanned downtime to investigate OOS instances can impact productivity. Our range of clone phases offer a cost-effective comparable alternative to many of the older leading brands, whilst ensuring consistency and stability in analysis. Improved lot-to-lot reproducibility is also achieved due to more stable production methods especially when compared to older brands.

The HarmonyTM C18 provides equivalent selectivity to the Waters Symmetry® C18 HPLC column and many chromatographers use Harmony when looking for a Symmetry equivalent. Harmony offers excellent column lifetime and reproducibility, and is engineered to the tightest specifications. It is also available in large scale bulk. Other Harmony phases are also available including C8 and C4.

This technical note provides examples of comparative studies between our Harmony C18 and the Waters Symmetry C18 phases for the following pharmaceutical drug applications:

  • Fluconazole
  • Betamethasone dipropionate and beclomethasone dipropionate
  • Acetaminophen, aspirin, caffeine and benzoic acid

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PerkinElmer LC 300 Autosampler Intermediate Loop Decompression (ILD) Injection Valve

The injection of an accurate and reproducible sample volume into a UHPLC system is challenging, but essential to ensuring a high quality analysis. The patented PerkinElmer Intermediate Loop Decompression (ILD) valve, featured in the LC 300 Autosampler, is a 7-port valve with a strategically placed radial groove in the rotor seal which ensures the injection of an accurate and reproducible sample volume, while also increasing the lifetime of columns used in analysis.

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