PerkinElmer
Analysis of Sugars in Maple Syrup by HILIC with Refractive Index Detection

Application Note

Analysis of Sugars in Maple Syrup by HILIC with Refractive Index Detection

Analysis of Sugars in Maple Syrup by HILIC with Refractive Index Detection

Introduction

Maple syrup, a common natural sweetener, is consumed regularly by households around the world. Quantification of sugar content in maple syrup is paramount to satisfy product labeling requirements and ensure label-claim accuracy. Although sucrose is the primary sugar component in maple syrup, a complete identification and quantification of the other complex carbohydrates, including glucose, fructose and maltose, is essential.

In this application note, a robust and efficient method for the determination of the four aforementioned complex carbohydrates is presented. Utilizing an LC 300 HPLC system, a hydrophilic interaction chromatography (HILIC) technique is utilized with subsequent detection achieved utilizing an LC 300 Refractive Index (RI) detector.