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LC 300 HPLC System

The PerkinElmer LC 300 platform redefines the liquid chromatography experience, offering the flexibility, performance and efficiency needed to tackle even the most challenging analytical demands. With the option to customize your experience with a wide range of detectors and accessories, each LC 300 system is designed to meet the most important specifications – yours. Paired with SimplicityChrom CDS software, the LC 300 HPLC system delivers an elevated user experience with simplified liquid chromatography workflows, intuitive functions, and automated processes.

Part Number N2550110
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The PerkinElmer LC 300 HPLC system has been designed to increase laboratory productivity and throughput, even in the face of challenging budget and staffing situations. Paired with the highly intuitive and powerful SimplicityChrom chromatography data system (CDS) software, our LC 300 systems are the ideal solution for labs seeking to maximize their liquid chromatography experience.

The LC 300 offers superior performance and flexibility throughout its intelligent design:

  • Robust and precise autosampler with integrated column oven and preheater, as well as a high visibility color LCD display showing full instrument system status, current queue and sample progress.
  • Optional external Peltier column oven for ultra-precise heating and cooling, for sample integrity and exceptionally accurate results.
  • Patented Intermediate Loop Decompression (ILD™) valve ensures the injection of an accurate and reproducible sample volume, while also increasing the lifetime of columns used in analysis.
  • Automated, onboard sample dilution and derivatization capabilities simplify the front-end analytical processes, while also ensuring reproducibility and precision.
  • 10k psi solvent delivery pump for high-end HPLC functionality, delivering repeatable solvent delivery at a wide range of flow rates and pressures.
  • Automatic priming design allows the system to achieve full self-priming capabilities, automating the otherwise cumbersome priming process utilized in many other systems.
  • Built-in solvent degassing technology simplifies the analytical process, and obviates the need for a separate, external degassing module.
  • Portfolio of five available detectors, which can be used individually or in sequence.
    • Photodiode array (PDA)
    • Multiwavelength UV/Vis (MWD)
    • Single wavelength UV/Vis
    • Refractive index (RI)
    • Fluorescence (FL)
  • Efficient and powerful instrument control with SimplicityChrom CDS, allowing users to create custom workflows and experiences, all while achieving compliance with 21 CFR Part 11 requirements.


Depth 22 inches

38.2 inches maximum (single stack with detector and standalone solvent organizer)


183 pounds (single stack with one detector and standalone solvent organizer) or 260 pounds maximum (dual stack with external oven)


13.5 inches maximum per component

Resources, Events & More
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Application Brief

Determination of Volatile Organic Compound (VOC) Metabolites in Urine by HPLC with UV Detection

Volatile organic compounds (VOCs) are compounds that can evaporate at ambient temperatures. They are emitted from certain solids or liquids and are prevalent in the environment. VOCs are present in homes and workplaces, originating from many sources, including household and industrial products, o ...

PDF 642 KB
Ferulic Acid Analysis in Angelica Sinensis Radix by HPLC

Angelica sinensis (A. sinensis) is a common health food and drug in Asia and utilized as a dietary supplement in women’s care in Europe. Nearly 20% of the traditional Chinese medicinal preparations included in the Chinese Pharmacopeia contain A. sinensis. Many bioactive constituents including pol ...

PDF 512 KB
Tanshinone and Derivatives Analysis in Radix Salviae Miltorrhizae by HPLC

Radix Salviae Miltorrhizae, called Danshen in Chinese Traditional Medicine (TCM), has been used to treat cardiovascular disease for more than a millennium. It is the root and rhizomes of the plant Salvia Miltiorrhiza Bunge of the Lamiaceae family. Pharmacological studies showed that nonpolar comp ...


Application Note

A Fast, Simple and Green Chemistry Method for the Analysis of Additives in Diet Soft Drinks by HPLC

Soft drink products serve as a major revenue stream for food and beverage manufacturers, with a global market size of USD 237 billion in 2021. Due to the popularity and wide consumption of these products, product consistency, which drives customer satisfaction, is of the utmost importance. For qu ...

PDF 594 KB
Analysis of 16 Cannabinoids Using the PerkinElmer LC 300 HPLC System with PDA Detection

Accurate identification and quantification of cannabinoid content is a critical step in the process to bringing safe and high quality cannabis flower and fortified products to market. As the legalization of recreational cannabis use continues throughout the world, fast and efficient liquid chroma ...

Analysis of Common Preservatives in Personal Care Products by HPLC with UV Detection

Parabens are utilized as an antimicrobial preservative in many commercial offerings including cosmetics, personal care products and foods, and offer both chemical and thermal stability to products. Although parabens increase the safety of products, recent studies have suggested that parabens may ...

Analysis of Phytoestrogen Isoflavones in Dietary Supplements by HPLC/UV

Isoflavones are water-soluble compounds found in many plant and food sources. Designated as phytoestrogens, isoflavones are structurally similar to to the female hormone estrogen, and have been shown to exhibit a number of beneficial impacts to human health, such as a reduction in the developmen ...

Analysis of Saccharides in High Fructose Corn Syrup

High fructose corn syrup (HFCS) as a substitute for sucrose in a wide variety of products, including baked goods, sauces, beverages, canned fruits and dairy products. HFCS is produced from regular corn syrup, with an enzymatic process that isomerizes some of the corn syrup's glucose into fructose ...

Analysis of Sugars in Maple Syrup by HILIC with Refractive Index Detection

Maple syrup, a common natural sweetener, is consumed regularly by households around the world. Quantification of sugar content in maple syrup is paramount to satisfy product labeling requirements and ensure label-claim accuracy. Although sucrose is the primary sugar component in maple syrup, a co ...

HPLC Analysis of Phenolic Antioxidants in Edible Fats and Oils supporting Food Quality Control and Label Transparency

Fats and oils are used in cooking as a frying medium or as an ingredient in baking. Autoxidation of lipids in the fats and oils proceeds as a free-radical chain reaction that leads to rancidity causing foul flavors and smells. Phenolic antioxidants may be added to fats and oils to prevent oxidati ...



Chromatography Solutions for Soft Drink Analysis

Monitoring the consistency and quality in soft drinks is essential to ensure a positive brand image and a loyal customer base. Our full portfolio of chromatography solutions, including LC, GC and GC/MS, enables you to meet – and even exceed – your quality control goals with rapid, intuitive, and ...

Comprehensive Testing Solutions for Feed Millers

Feed milling today is a complex business, and agribusinesses both large and small need to balance the nutritional and safety needs of livestock with availability of raw ingredients and their seasonality and variability. Add to that the valuable supplementation and medication that millers supply, ...

LC 300 HPLC and UHPLC Systems Interactive Brochure

Laboratories in nearly every industry are continuously faced with growing demands for increased throughput, improved margins, and more efficient processes. And when it comes to liquid chromatography, a one-size-fits-all approach just isn’t enough. You need an LC system tailored to your needs, wit ...


Case Study

PFAS Monitoring: How One Scientist is Thinking Ahead for the Environment

Predicting the long-term impact some substances have on human health and the environment is extremely difficult and in some instances we only become aware of certain substances and chemicals’ toxic impact after they have been widely used.

Recently, PFAS (per- and polyfluoroalkyl substances) ...

PDF 341 KB


PerkinElmer Soft Drink Additives Analysis Workflow Solution

Beverage bottling plants must ensure raw materials and finished products are both consistent and safe, requiring a variety of quality control analytical testing. For the analysis of additives in syrups and/or finished products – whether sweeteners, preservatives or caffeine – PerkinElmer offers a ...

SimplicityChrom CDS

SimplicityChrom is a modern, intuitive and flexible CDS software platform that enables users to efficiently control their instrument, process data, and generate results. Pair this functionality with the peace of mind of 21 CFR Part 11 compliance and industry-leading service and ...


Product Note

LC 300 Product Note

The PerkinElmer LC 300 liquid chromatography systems are the result of listening to user needs, and paying careful attention to every detail in the resulting design. The LC 300 systems are the ideal solution for labs faced with high throughput requirements or challenging chromatographic separatio ...

PDF 950 KB

Technical Note

PerkinElmer LC 300 Autosampler Intermediate Loop Decompression (ILD) Injection Valve

The injection of an accurate and reproducible sample volume into a UHPLC system is challenging, but essential to ensuring a high quality analysis. The patented PerkinElmer Intermediate Loop Decompression (ILD) valve, featured in the LC 300 Autosampler, is a 7-port valve with a strategically place ...

PDF 849 KB

White Paper

CASE Chemicals: Trends, Innovations, and Challenges

The specialty chemical category referred to as CASE – coatings, adhesives, sealants, and elastomers – are essential materials throughout industrial and consumer product manufacturing. CASE provide advanced functional characteristics – e.g. performance, protection, operation, strength, and durabil ...

PDF 293 KB