LC 300 HPLC System

Accurate identification and quantification of cannabinoid content is a critical step in the process to bringing safe and high quality cannabis flower and fortified products to market. As the legalization of recreational cannabis use continues throughout the world, fast and efficient liquid chromatography methods for the determination of cannabinoid content are need to satisfy both regulatory and consumer demands.

In this application note, a fast and simple method for the determination of 16 common cannabinoids is presented. Utilizing a PerkinElmer LC 300 HPLC system with a PDA detector, the method results in the elution of all sixteen compounds in under seven minutes, with LOQs = 0.15 µg/mL for most analytes.

Parabens are utilized as an antimicrobial preservative in many commercial offerings including cosmetics, personal care products and foods, and offer both chemical and thermal stability to products. Although parabens increase the safety of products, recent studies have suggested that parabens may exhibit weak endocrine disrupting effects on users of the products, an effect that can be further intensified by the ability of parabens to be absorbed by the skin without being broken done by esterase enzymes.

In this application note, a fast, robust and sensitive method utilizing a PerkinElmer LC 300 HPLC system with Multi-Wavelength UV/Vis (MWD) detector for the analysis of 2-phenoxyethanol and seven commonly used parabens in eight different commercially available cosmetic and personal care products is presented.

Isoflavones are water-soluble compounds found in many plant and food sources. Designated as phytoestrogens, isoflavones are structurally similar to to the female hormone estrogen, and have been shown to exhibit a number of beneficial impacts to human health, such as a reduction in the development of certain cancers, as well as an improvement in cholesterol ratios. Studies also suggest, however, that the phytoestrogenic nature of isoflavones can also lead to an exacerbation of existing thyroid disorders, owing to a disruption to the endocrine system. As such, precise quantification of isoflavone content in nutraceutical products is paramount to ensure label-claim accuracy and consumer safety.

In this application note, an efficient and reliable method for the determination of six common isoflavone compounds is presented. The method, which was developed in accordance with the USP monograph for soy isoflavones in dietary supplement capsules, utilizes a PerkinElmer LC 300 UHPLC system, with subsequent detection of compounds achieved utilizing an LC 300 PDA detector.

High fructose corn syrup (HFCS) as a substitute for sucrose in a wide variety of products, including baked goods, sauces, beverages, canned fruits and dairy products. HFCS is produced from regular corn syrup, with an enzymatic process that isomerizes some of the corn syrup's glucose into fructose. The fructose concentration can vary, depending upon the intended use, and is commonly produced at concentrations of 42% (HFCS-42), 55% (HFCS-55) and 90% (HFCS-90).

The US Food and Drug Administration Code of Federal Regulations have incorporated identity standards for the sugar composition of HFCS, specifically around fructose content where HFCS-42 cannot be less than 42% fructose and HFCS-55 cannot be less than 55% fructose. This application note demonstrates the suitability of the PerkinElmer LC 300 HPLC system for performing saccharide content determination via method CRA SACCH.03 through the analysis of nine commercially available high fructose corn syrups.

Maple syrup, a common natural sweetener, is consumed regularly by households around the world. Quantification of sugar content in maple syrup is paramount to satisfy product labeling requirements and ensure label-claim accuracy. Although sucrose is the primary sugar component in maple syrup, a complete identification and quantification of the other complex carbohydrates, including glucose, fructose and maltose, is essential.

In this application note, a robust and efficient method for the determination of the four aforementioned complex carbohydrates is presented. Utilizing an LC 300 HPLC system, a hydrophilic interaction chromatography (HILIC) technique is utilized with subsequent detection achieved utilizing an LC 300 Refractive Index (RI) detector.

Feed milling today is a complex business, and agribusinesses both large and small need to balance the nutritional and safety needs of livestock with availability of raw ingredients and their seasonality and variability. Add to that the valuable supplementation and medication that millers supply, and it’s clear: you’re creating a complete nutritional delivery system, not just a simple mix of grains.

Laboratories in nearly every industry are continuously faced with growing demands for increased throughput, improved margins, and more efficient processes. And when it comes to liquid chromatography, a one-size-fits-all approach just isn’t enough. You need an LC system tailored to your needs, with user-friendly software, consumables, service, and support – the total package.

Meet the PerkinElmer LC 300, the latest LC to step off our production lines. With features and benefits such as five available detectors, ultraprecise gradient flows, low dispersion, and new Simplicity^™ Chrom CDS software, our LC 300 systems provide improved workflows, throughput, and usability.

SimplicityChrom^™ is a modern, intuitive and flexible CDS software platform that enables users to efficiently control their instrument, process data, and generate results. Pair this functionality with the peace of mind of 21 CFR Part 11 compliance and industry-leading service and support, and you’re free to focus on what matters most – your analytical goals.

The PerkinElmer LC 300 liquid chromatography systems are the result of listening to user needs, and paying careful attention to every detail in the resulting design. The LC 300 systems are the ideal solution for labs faced with high throughput requirements or challenging chromatographic separations.

Whatever the application, the PerkinElmer LC 300 system’s performance and enhanced user experience will ensure your lab’s success, whether that need be for pharmaceutical, food, consumer products or specialty chemicals.

In this product note, detailed information on each of the LC 300's modules is presented.

The injection of an accurate and reproducible sample volume into a UHPLC system is challenging, but essential to ensuring a high quality analysis. The patented PerkinElmer Intermediate Loop Decompression (ILD) valve, featured in the LC 300 Autosampler, is a 7-port valve with a strategically placed radial groove in the rotor seal which ensures the injection of an accurate and reproducible sample volume, while also increasing the lifetime of columns used in analysis.