check quantity

LC 300 HPLC System

The PerkinElmer LC 300 platform redefines the liquid chromatography experience, offering the flexibility, performance and efficiency needed to tackle even the most challenging analytical demands. With the option to customize your experience with a wide range of detectors and accessories, each LC 300 system is designed to meet the most important specifications – yours. Paired with SimplicityChrom CDS software, the LC 300 HPLC system delivers an elevated user experience with simplified liquid chromatography workflows, intuitive functions, and automated processes.

Part Number N2550110
Buy Now

Please enter valid quantity

Please log in to add favorites.



The PerkinElmer LC 300 HPLC system has been designed to increase laboratory productivity and throughput, even in the face of challenging budget and staffing situations. Paired with the highly intuitive and powerful SimplicityChrom chromatography data system (CDS) software, our LC 300 systems are the ideal solution for labs seeking to maximize their liquid chromatography experience.

The LC 300 offers superior performance and flexibility throughout its intelligent design:

  • Robust and precise autosampler with integrated column oven and preheater, as well as a high visibility color LCD display showing full instrument system status, current queue and sample progress.
  • Optional external Peltier column oven for ultra-precise heating and cooling, for sample integrity and exceptionally accurate results.
  • Patented Intermediate Loop Decompression (ILD™) valve ensures the injection of an accurate and reproducible sample volume, while also increasing the lifetime of columns used in analysis.
  • Automated, onboard sample dilution and derivatization capabilities simplify the front-end analytical processes, while also ensuring reproducibility and precision.
  • 10k psi solvent delivery pump for high-end HPLC functionality, delivering repeatable solvent delivery at a wide range of flow rates and pressures.
  • Automatic priming design allows the system to achieve full self-priming capabilities, automating the otherwise cumbersome priming process utilized in many other systems.
  • Built-in solvent degassing technology simplifies the analytical process, and obviates the need for a separate, external degassing module.
  • Portfolio of five available detectors, which can be used individually or in sequence.
    • Photodiode array (PDA)
    • Multiwavelength UV/Vis (MWD)
    • Single wavelength UV/Vis
    • Refractive index (RI)
    • Fluorescence (FL)
  • Efficient and powerful instrument control with SimplicityChrom CDS, allowing users to create custom workflows and experiences, all while achieving compliance with 21 CFR Part 11 requirements.


Depth 22 inches

38.2 inches maximum (single stack with detector and standalone solvent organizer)


183 pounds (single stack with one detector and standalone solvent organizer) or 260 pounds maximum (dual stack with external oven)


13.5 inches maximum per component

Resources, Events & More
  • All

Application Note

Analysis of 16 Cannabinoids Using the PerkinElmer LC 300 HPLC System with PDA Detection

Accurate identification and quantification of cannabinoid content is a critical step in the process to bringing safe and high quality cannabis flower and fortified products to market. As the legalization of recreational cannabis use continues throughout the world, fast and efficient liquid chromatography methods for the determination of cannabinoid content are need to satisfy both regulatory and consumer demands.

In this application note, a fast and simple method for the determination of 16 common cannabinoids is presented. Utilizing a PerkinElmer LC 300 HPLC system with a PDA detector, the method results in the elution of all sixteen compounds in under seven minutes, with LOQs = 0.15 µg/mL for most analytes.

Analysis of Common Preservatives in Personal Care Products by HPLC with UV Detection

Parabens are utilized as an antimicrobial preservative in many commercial offerings including cosmetics, personal care products and foods, and offer both chemical and thermal stability to products. Although parabens increase the safety of products, recent studies have suggested that parabens may exhibit weak endocrine disrupting effects on users of the products, an effect that can be further intensified by the ability of parabens to be absorbed by the skin without being broken done by esterase enzymes.

In this application note, a fast, robust and sensitive method utilizing a PerkinElmer LC 300 HPLC system with Multi-Wavelength UV/Vis (MWD) detector for the analysis of 2-phenoxyethanol and seven commonly used parabens in eight different commercially available cosmetic and personal care products is presented.

Analysis of Phytoestrogen Isoflavones in Dietary Supplements by HPLC/UV

Isoflavones are water-soluble compounds found in many plant and food sources. Designated as phytoestrogens, isoflavones are structurally similar to to the female hormone estrogen, and have been shown to exhibit a number of beneficial impacts to human health, such as a reduction in the development of certain cancers, as well as an improvement in cholesterol ratios. Studies also suggest, however, that the phytoestrogenic nature of isoflavones can also lead to an exacerbation of existing thyroid disorders, owing to a disruption to the endocrine system. As such, precise quantification of isoflavone content in nutraceutical products is paramount to ensure label-claim accuracy and consumer safety.

In this application note, an efficient and reliable method for the determination of six common isoflavone compounds is presented. The method, which was developed in accordance with the USP monograph for soy isoflavones in dietary supplement capsules, utilizes a PerkinElmer LC 300 UHPLC system, with subsequent detection of compounds achieved utilizing an LC 300 PDA detector.

Analysis of Saccharides in High Fructose Corn Syrup

High fructose corn syrup (HFCS) as a substitute for sucrose in a wide variety of products, including baked goods, sauces, beverages, canned fruits and dairy products. HFCS is produced from regular corn syrup, with an enzymatic process that isomerizes some of the corn syrup's glucose into fructose. The fructose concentration can vary, depending upon the intended use, and is commonly produced at concentrations of 42% (HFCS-42), 55% (HFCS-55) and 90% (HFCS-90).

The US Food and Drug Administration Code of Federal Regulations have incorporated identity standards for the sugar composition of HFCS, specifically around fructose content where HFCS-42 cannot be less than 42% fructose and HFCS-55 cannot be less than 55% fructose. This application note demonstrates the suitability of the PerkinElmer LC 300 HPLC system for performing saccharide content determination via method CRA SACCH.03 through the analysis of nine commercially available high fructose corn syrups.

Analysis of Sugars in Maple Syrup by HILIC with Refractive Index Detection

Maple syrup, a common natural sweetener, is consumed regularly by households around the world. Quantification of sugar content in maple syrup is paramount to satisfy product labeling requirements and ensure label-claim accuracy. Although sucrose is the primary sugar component in maple syrup, a complete identification and quantification of the other complex carbohydrates, including glucose, fructose and maltose, is essential.

In this application note, a robust and efficient method for the determination of the four aforementioned complex carbohydrates is presented. Utilizing an LC 300 HPLC system, a hydrophilic interaction chromatography (HILIC) technique is utilized with subsequent detection achieved utilizing an LC 300 Refractive Index (RI) detector.



Comprehensive Testing Solutions for Feed Millers

Feed milling today is a complex business, and agribusinesses both large and small need to balance the nutritional and safety needs of livestock with availability of raw ingredients and their seasonality and variability. Add to that the valuable supplementation and medication that millers supply, and it’s clear: you’re creating a complete nutritional delivery system, not just a simple mix of grains.

LC 300 HPLC and UHPLC Systems Interactive Brochure

Laboratories in nearly every industry are continuously faced with growing demands for increased throughput, improved margins, and more efficient processes. And when it comes to liquid chromatography, a one-size-fits-all approach just isn’t enough. You need an LC system tailored to your needs, with user-friendly software, consumables, service, and support – the total package.

Meet the PerkinElmer LC 300, the latest LC to step off our production lines. With features and benefits such as five available detectors, ultraprecise gradient flows, low dispersion, and new Simplicity Chrom CDS software, our LC 300 systems provide improved workflows, throughput, and usability.



SimplicityChrom CDS

SimplicityChrom is a modern, intuitive and flexible CDS software platform that enables users to efficiently control their instrument, process data, and generate results. Pair this functionality with the peace of mind of 21 CFR Part 11 compliance and industry-leading service and support, and you’re free to focus on what matters most – your analytical goals.


Product Note

LC 300 Product Note

The PerkinElmer LC 300 liquid chromatography systems are the result of listening to user needs, and paying careful attention to every detail in the resulting design. The LC 300 systems are the ideal solution for labs faced with high throughput requirements or challenging chromatographic separations.

Whatever the application, the PerkinElmer LC 300 system’s performance and enhanced user experience will ensure your lab’s success, whether that need be for pharmaceutical, food, consumer products or specialty chemicals.

In this product note, detailed information on each of the LC 300's modules is presented.

PDF 950 KB

Technical Note

PerkinElmer LC 300 Autosampler Intermediate Loop Decompression (ILD) Injection Valve

The injection of an accurate and reproducible sample volume into a UHPLC system is challenging, but essential to ensuring a high quality analysis. The patented PerkinElmer Intermediate Loop Decompression (ILD) valve, featured in the LC 300 Autosampler, is a 7-port valve with a strategically placed radial groove in the rotor seal which ensures the injection of an accurate and reproducible sample volume, while also increasing the lifetime of columns used in analysis.

PDF 849 KB