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  • Application Note

    High Sensitivity Methotrexate Quantitation in Human Serum Utilizing

    This application note outlines a rapid LC-MS/MS research method utilizing the QSight® 220 triple quadrupole mass spectrometer. The developed method provides exceptional results for the quantitation of methotrexate in serum especially in terms of LLOQs and linearity.

  • Brochure

    QSight Pesticides Analyzer Brochure

    Pesticides can adversely affect human health through the food we consume but can also leach into soil and groundwater and impact us through the environment. To keep up with a complex and constantly changing regulatory environment, your pesticide analysis solution needs to keep up with your burgeoning sample load.

  • Application Note

    Analysis of Pesticide Residues in Cannabis Regulated by Oregon State Using LC/MS/MS

    Liquid chromatography-tandem mass spectrometry (LC/MS/MS) has become the method of choice for pesticide The state of Oregon has issued regulatory limits for 59 pesticide residues in both cannabis flower and concentrates, while other states have come up with

  • Specification Sheet

    Quasar SPP Particle Dimensions Specification Sheet

    Our Quasar portfolio of LC columns allows you to achieve rugged and reproducible results – batch to batch and column to column – with an all-encompassing, flexible solution that meets the diverse, changing needs of analysis.

  • Application Brief

    Rapid LC Analysis of Cephalosporins Using Quasar Biphenyl Column

    The aerobic mold which yielded cephalosporin C was found in the sea near a sewage outfall nearby Cagliari harbour, Sardinia, by the Italian pharmacologist Giuseppe Brotzu in July 1945. Since their discovery and subsequent commercialization in 1964, the cephalosporins today are broad-spectrum ß-lactam antibiotics used for the treatment of a number of bacterial conditions including septicaemia, pneumonia and meningitis. The pharmacology of cephalosporins is similar to that of the penicillin class of compounds. This application brief describes use of a Quasar biphenyl column in the analysis of several cephalosporins, a mixture of first and second generation ß-lactam antibiotics.

  • Application Note

    Analysis of Aflatoxins by UHPLC

    Aflatoxin B1 is considered to be the most genotoxic of the mycotoxins, and, when ingested by cows, is converted to aflatoxin M1 which has been shown to cause liver cancer in certain animals.

  • Application Note

    Determination of the Nitrofuran Metabolites in Seafood using Ultra High-Performance Liquid Chromatography - Tandem Mass Spectrometry

    Nitrofurans are a class of broad-spectrum antibiotics that are widely used to kill or slow down the growth of bacteria in the aquaculture industry. The use of nitrofurans and their metabolites has been restricted by a number of countries and organizations within the European Union, United States and China, due to their harmful side effects to human health, and a Minimum Required Performance Limit (MRPL) 0f 1.0 µg/kg has been set for food, animal and aquaculture products.

    LC/MS/MS methods have been used for the detection of nitrofuran metabolites in many studies owing to the high sensitivity, selectivity and specificity of the methods. However, nitrofuran metabolites demonstrate low ionization efficiency with the electrospray ion source used in LC/MS/MS systems. Therefore, the use of a derivatization step for nitrofuran metabolites before extraction and LC/MS/MS analysis is crucial to meet the low regulatory limits of 1.0 µg/kg in food samples. The main purpose of this work is to determine the detection limits for nitrofurans using QSight® 210 LC/MS/MS system.

  • Application Brief

    Analysis of Water-Soluble Vitamins (B2, B3) using Quasar SPP HILIC Column

    In 1906, English biochemist Sir Frederick Gowland Hopkins also discovered that certain food factors were important to health. All the vitamins we recognise today were discovered during the early 20th century. There are two groups of vitamins, fat soluble and water soluble. Both types are regarded as essential for normal growth and our overall well being. Vitamin B2 (Riboflavin) and vitamin B3 (Niacinamide) are water soluble vitamins, naturally occurring in a number of food stuffs including milk and eggs. Both vitamins are used as dietary supplements and can help lower cholesterol, prevent migraines. Hydrophilic interaction liquid chromatography (HILIC), as first suggested by Alpert in 1990 provides an alternative approach to effectively separate small polar compounds, on polar stationary phases, which are not well retained under reversed phase conditions. Water is the strongest solvent in HILIC and as mobile phases typically contain low levels of water the resulting methods are ideally suited for MS applications. This application brief illustrates the efficient separation of vitamin B2 and B3 applying the HILIC mode of separation using the Quasar SPP HILIC column.

  • Application Note

    Augmenting Standard Methods of Measuring Airborne PM2.5 Using IR Imaging

    The use of IR imaging expands the measurement possibilities by giving information about the individual types of materials present in the collected particles. The information from the IR imaging experiment is not only qualitative (i.e. identification of the particulates), but can also be calibrated to give quantitative information on the components present. The IR imaging measurement will take only five minutes, whereas Ion Chromatography requires solvent extraction of the particulates and would take around 20-25 minutes per sample

  • Application Brief

    Robust Atorvastatin Analysis Under High Aqueous LC Conditions Application Brief

    The origin of statins dates back to the mid 1970’s when the Japanese biochemist, Akira Endo, isolated a factor from the fungus Penicillium citrinum which he identified as a competitive inhibitor of 3-hydroxy-3-methylglutarylcoenzyme A reductase (HMG-CoA reductase). This substance, which he named compactin or mevastatin, was the first statin to be administered to humans. Statins competitively inhibit HMG-CoA reductase, an enzyme involved in cholesterol synthesis, especially in the liver. Used to prevent cardiovascular disease and to lower cholesterol levels, atorvastatin is one of a number of synthetic statins routinely produced. It is now the most commonly prescribed statin medication. This application brief describes the robust HPLC analysis of the polar compound atorvastatin using a gradient which reaches 100% aqueous mobile phase conditions.

  • Brochure

    Quasar Liquid Chromatography Columns Brochure

    Whether you’re testing food, water, or pharmaceuticals, success often depends on getting back to basics. So whatever your separation challenge, your choice of liquid chromatography (LC) column can make all the difference. Our Quasar portfolio of LC columns allows you to achieve rugged and reproducible results – batch to batch and column to column – with an all-encompassing, flexible solution that meets the diverse, changing needs of analysis. Ultrapure silica-based Quasar columns deliver a comprehensive range of chemistries, together with state-of-the art, optimized bonding technology to give you a versatile, high-performing analytical solution for your increasingly complex samples. For flexibility, we provide a wide range of column sizes, including shorter columns packed with smaller particle sizes for shorter run times and better productivity. Plus, our scalable columns facilitate easy method transfer between HPLC and UHPLCtechnology platforms – and the smaller particle sizes means optimized sensitivity for those applications. Whatever your separation need, we have a chemistry or dimension to fill it.

  • Application Note

    Simultaneous Analysis of Nine Food Additives with the PerkinElmer Flexar FX-15 System Equipped with a PDA Detector

    This application note presents a fast and robust liquid chromatography method to simultaneously test nine widely used additives. Among the additives tested are: preservatives (benzoic acid, sorbic acid, dehydroacetic acid and methylparaben); artificial sweeteners (acesulfame potassium, saccharin and aspartame); flavoring agent (quinine); and a stimulant (caffeine).

  • Application Brief

    Amoxicillin Analysis Using Quasar C18 HPLC Column

    Antibiotics are natural substances released by bacteria and fungi which are capable of killing, or inhibiting, competing microbial species. This phenomenon has long been known; but it was not until 1928 that penicillin, the first true antibiotic, was discovered by Alexander Fleming, Professor of Bacteriology at St. Mary's Hospital in London. However, it was 12 years later that penicillin was isolated and developed as a medicine by Howard Florey and Ernst Chain. Since this breakthrough in therapeutic medicine, derivates of penicillin were developed with increased efficacy levels. Amoxicillin is one of several such semisynthetic penicillin’s. First discovered in the 1960’s its patent has now expired. Consequently, amoxicillin and co-amoxiclav is now marketed under many different trade names worldwide. It is commonly used in the treatment of numerous infections including pneumonia, skin infections and Lyme disease. This application brief illustrates the rapid analysis of amoxicillin using the Quasar C18 column.

  • Application Brief

    HPLC Analysis of Plavix Using Quasar C8 Column

    The medication known as Plavix is an antiplatelet drug which prevents platelets in your blood from sticking together to form an unwanted blood clot, that could block an artery. It is used to lower the risk of having a stroke, or serious heart problem after you've had a heart attack, or aid with circulation problems as it helps to keep blood flowing smoothly in the body. The patent expired in 2012 and the drug is now also sold under many generic brands. Plavix tablets contain the active ingredient clopidogrel hydrogen sulphate, which is readily analysed by HPLC. This application brief describes use of a Quasar C8 column in the analysis of Plavix.

  • Application Brief

    HPLC Analysis of Vitamin C Using Quasar Biphenyl Column

    Vitamin C, also known as ascorbic acid and L-ascorbic acid, is a water soluble vitamin found in various foods including peppers, kiwifruit, oranges and kale. It is regarded as an essential nutrient to prevent scurvy, involved in the repair of tissue and also thought to lower cancer risk. It is required for the functioning of several enzymes and is important for immune system function. Since it’s isolation in 1928 it was the first vitamin to be commercially produced. Today it is widely available as a dietary supplement. This application brief describes use of a Quasar biphenyl column in the analysis of vitamin C.

  • Application Note

    Rapid UHPLC Determination of Nine Common Herbicides in Drinking Water with the PerkinElmer Flexar FX

    Water polluted by herbicides leach and runoff can cause human health problems including cancer tumors, reproduction deformity, disruption of the endocrine system and DNA damage. This application presents a sensitive and robust liquid chromatography method to test nine widely used herbicides (Figure 1), using a 3 µm UHPLC column to achieve very high throughput at a low flow rate to reduce testing time and solvent consumption. The throughput is compared to that of a conventional C18 HPLC column. Method conditions and performance data including precision and linearity, are presented.

  • Poster

    Quasar Liquid Chromatography Columns Postcard

    INTRODUCTORY TRIAL OFFER: 25% OFF DISCOUNT on all Quasar LC Columns Until June 30, 2019 To take advantage of this offer, visit our website and use promotion code: AMSLC19 at checkout.

  • Application Note

    Cannabis Analysis: Potency Testing Identification and Quantification of THC and CBD by GC/FID and GC/MS

    This application note will concentrate on the potency identification and quantification of THC and CBD in cannabis by Gas Chromatography. Other application notes will cover potency by HPLC, pesticide analysis and residual solvent analyses. Analysis of cannabis has taken on new importance in light of legalized marijuana in several states of the USA. Cannabis contains several different components classed as cannabinoids. Primary cannabinoids of interest are tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN). Positive identification and quantification of the THC/CBD ratio is a primary objective in the analysis of cannabis. Cannabis is analyzed for several different purposes.

  • Application Brief

    Warfarin Analysis Using a Quasar AQ UHPLC Column

    Today, Warfarin is the most widely used anticoagulant in the world, used to thin the blood and prevent clots.1 It was discovered by chance when in the 1920’s cattle in the US were found to be bleeding to death having eaten mouldy hay from sweet clover crops.2 However, the exact identity of the substance causing the haemorrhaging was to remain unknown for many years. Over the coming years studies of the spoiled hay eventually led to the extraction of a compound which was later named dicoumarol. It was observed that this dicoumarol could not act as an anticoagulant on its own. It was only after it was metabolised byfungi that it exhibited anticoagulant properties. This explained why only spoiled hay caused the outbreak in the cattle. After further research, the synthesis of a more potent anticoagulant from dicoumarol, warfarin, was produced. Warfarin first commercial use was as a rat poison in 1948, followed by license for human use in 1953. This application brief illustrates the analysis of warfarin, Figure 1, using the Quasar AQ liquid chromatography phase.

  • Flyer

    Quasar Liquid Chromatography Columns Flyer

    Our wide range of column sizes has its benefits, including:• Longevity to withstand highthroughput environments• High-efficiency separations to facilitate trace-level analysis• Good resolution and peak shapes for more effective compound separation• Analysis of increasing polar and complex analytes.

  • Application Note

    Pesticides and Organic Toxins Compendium

    A Compendium of Pesticides and Organic Toxins Application Notes: Rice, oatmeal, wine, berries, corn and multi-grain cereals.

  • Application Brief

    Analysis of Naproxen using Quasar SPP C18 Column

    Naproxen is a nonsteroidal anti-inflammatory drugs (NSAIDs) used to treat certain types of arthritis and other acute inflammatory conditions. It was first released to the prescription drug market in 1976 under the name Naprosyn. Then a few years later its counterpart salt, naproxen sodium, was released for prescription use and is used predominately in formulations today. The HPLC analysis of Naproxen on a porous silica C18 column is well documented in the literature. However, with the current trend in liquid chromatography being towards higher kinetic efficiency and shorter analysis time the subsequent development in column technology has realised that, without the need to change instrumentation. This application brief will illustrate the application of a superficially porous particles (SPP) column for the analysis of naproxen.

  • Application Note

    Cannabinoid Monitoring in Dried Cannabis Flowers

    This application describes an analytical method for the chromatographic separation and quantitative monitoring of seven primary cannabinoids, including THC and THC-A, in cannabis extracts by HPLC with PDA detection. Naturally occurring cannabinoids, the main biologically active component of the cannabis plant, form a complex group of closely related compounds, of which 113 are known and 70 are well described. Of these, the primary focus has been on ?9-tetrahydrocannabinol (THC), as the primary active ingredient due to its pharmacological and toxicological characteristics, upon which strict legal limits have been enforced.

  • Application Note

    Analysis of Ginsenosides in Ginseng Root with the PerkinElmer Flexar FX-15 System Equipped with a PDA Detector

    Ginseng has been used as an herbal medicine in Asia for over two thousand years for its purported various health benefits, including antioxidant, anticarcinogenic, antiinflammatory, antihypertensive and anti-diabetic. The pharmacologically active compounds behind the claims of ginseng’s efficacy are ginsenosides; their underlying mechanism of action although not entirely elucidated appears to be similar to that of steroid hormones. There are a number of ginseng species, and each has its own set of ginsenosides.

  • Case Study

    A Better Way to Target Pesticides in Berries

    With the use of potentially harmful pesticides to protect berry crops on the rise, PerkinElmer introduces unique ways to detect and quantify pesticide residue in fruit.

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