PerkinElmer
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250 mm, 4.6 mm I.D., 5 Quasar SPP C18 LC Column

HILIC Quasar SPP HILIC LC columns encompass a uniform surface for improved reproducibility and yield excellent peak shapes for a wide range of compounds, including basic analytes.

Part Number
Particle Size
Length
Inner Diameter
List Price
Your Price
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N9308910
2.6 µm
150.0 mm
4.6 mm
785.00 USD
 
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N9308911
2.6 µm
100.0 mm
4.6 mm
773.00 USD
 
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N9308912
2.6 µm
50.0 mm
4.6 mm
762.00 USD
 
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N9308913
2.6 µm
150.0 mm
3 mm
785.00 USD
 
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N9308914
2.6 µm
100.0 mm
3 mm
773.00 USD
 
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N9308915
2.6 µm
50.0 mm
3 mm
572.00 USD
 
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N9308916
2.6 µm
150.0 mm
2.1 mm
706.00 USD
 
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N9308917
2.6 µm
100.0 mm
2.1 mm
639.00 USD
 
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N9308918
2.6 µm
50.0 mm
2.1 mm
572.00 USD
 
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N9308955
5.0 µm
250.0 mm
4.6 mm
762.00 USD
 
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N9308956
5.0 µm
150.0 mm
4.6 mm
695.00 USD
 
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N9308957
5.0 µm
100.0 mm
4.6 mm
650.00 USD
 
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N9308958
5.0 µm
50.0 mm
4.6 mm
605.00 USD
 
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Overview

Specifications

Inner Diameter 4.6 mm
Length 250.0 mm
Particle Size 5.0 µm
Product Brand Name Quasar
Type SPP C18
Resources, Events & More
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Application Brief

Optimizing the Separation of Four Tocopherols and Four Tocotrienols via RP-HPLC

The knowledge about tocochromanol-related compounds, especially tocotrienols, tocodienols,tocomonoenols, and others, is still limited due to several challenges faced in analytical chemistry. These challenges include separation resolution, co-elution, price and absence of standards, and low analyte concentration in plant material. Application of different column stationary phase chemistries can assist in the challenges faced in compound separation.

Superficially porous particle (SPP) columns can also be used to improve separation of these compounds. SPP particles are made of a solid, non-porous core surrounded by a shell of a porous silica material, resulting in a shorter diffusion path in comparison with fully porous based columns. With a shorter diffusion path within the SPP particle itself, coupled with a uniform packed bed and ultra-inert silica surface, reductions in run times can be observed. Such phases benefit from increased efficiency, with separation resembling that of a UHPLC column. They can be used on standard HPLC instrumentation, without concerns regarding high backpressures, which often compromise column longevity.

In this application brief, five different Quasar SPP column phases were screened for the separation of four tocopherol and four tocotrienol homologs, with focus on resolving β and γ isomers.

PDF 1 MB

Technical Note

A Comparison of Fully Porous C18 Reversed Phase HPLC Columns Technical Note

The most commonly used LC reversed-phase alkyl bonded stationary phase is octadecyl carbon chain (C18)-bonded silica, which is denoted as USP classification L1. C18 columns have a broad applicability from pharmaceuticals to food and environmental analyses. However, not all C18 columns are alike. Simply swapping a C18 column from one manufacturer to another can result in differences in retention time, resolution and even selectivity. Differences can arise due to variations in hydrophobicity, silanol activity, packing quality, particle size distribution, and silica purity, to name a few.

This technical note provides details of a comparative study between twenty-four silica-based C18 phases, from a number of manufacturers covering the following areas:

  • Relative Hydrophobicity Study
  • Efficiencies for a Neutral Compound
  • Evaluation of Silanol Activity
  • PDF 1 MB