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LAMBDA 465 UV/Vis Spectrophotometer

Designed specifically for high-end research as well as routine and high-throughput applications, the LAMBDA 465 UV/Vis is the innovative PDA solution that provides maximum reliability – for maximum confidence in your results.

Part Number N4100030
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Ultrafast, high-performance, reliable photodiode array UV Vis

Its PDA technology allows the acquisition of a full spectrum – from 1100 nm to 190 nm – in as little as 20 msec. In addition, the system has 1-nm Optical resolution, allowing it to meet the requirements of a number of pharmacopoeias. With 21 CFR part 11 compliant software, it’s an ideal solution for dissolution, fast kinetics, and other applications where high-speed scanning and high resolution are required – and it’s perfect for method development and sample analysis, too.

For flexibility in sampling along with high resolution and low noise spectrum, a dual light source (tungsten and deuterium) in a see-through configuration is unitized, providing the highest energy throughput possible – an important consideration with accessories that impact energy throughput.


21 CFR Part 11 Compatible Yes
Height 23.0 cm
Interface Tungsten-halogen and Deuterium
Maximum Temperature 35 °C
Minimum Temperature 15 °C
Model Name LAMBDA 465
Operating Range 190 - 1100 nm
Portable No
Product Brand Name LAMBDA
Research Areas Food & Agriculture
Warranty 1 year
Wave Length Range 190 - 1100 nm
Weight 16.0 kg
Width 45.0 cm
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Application Brief

Determination of Monoclonal Antibody Aggregation using UV/Vis Spectroscopy

Monoclonal antibodies are becoming more widespread as treatments for a variety of diseases including osteoporosis and various cancers. Furthermore, monoclonal antibodies have received emergency authorization for use as treatment for SARS-CoV-2, or COVID-19, from the FDA under the drug names casirivimab and imdevimab. Aggregation of antibodies may take place due to unwanted folding of the protein structure and can be influenced by a wide variety of factors including pH, presence of other free bases and, most importantly for this application, temperature.

Aggregation index provides a semi-quantitative view of the extent of aggregation in antibodies and can be determined by simply measuring the UV/Visible spectrum of the material. UV/Visible spectroscopy provides a fast method for estimation of the extent to which antibodies have become aggregated. This application brief describes how the PerkinElmer LAMBDA 465 with UVLab software allows users to measure samples in less than one minute.

PDF 985 KB

Application Note

Determination of Cholesterol Level in Human Serum - Enzymatic Colorimetric Method

Clinical chemistry uses chemical processes to measure levels of chemical components in the blood. It is very useful for the early diagnostic of disease and for monitoring organ function. The most common specimens used in clinical chemistry are blood and urine and this application note shows the common blood tests and measurable items using UV/Vis spectrophotometers as determined by the enzymatic method.

PDF 830 KB
Determination of Hydroxymethylfurfural (HMF) in Honey using the LAMBDA Spectrophotometer

The level of Hydroxymethylfurfural (HMF) present is a key indicator as to the quality of honey. In this application note, the HMF content has been determined using the LAMBDA 465 UV/Vis Spectrophotometer and the Equation Calculation mode in the UV Lab software.

Determination of Sugar as Glucose in a Soft Drink Using the LAMBDA PDA UV/Vis Spectrophotometer

In this application note, the amount of sugar or carbohydrate in a soft drink was determined using a colorimetric method. The rapid measurement of the PDA (Photodiode Array) UV/Vis Spectrophotometer allows for the collection of accurate data from the time-dependent reaction. The calibration curve was automatically calculated using the Quantification mode of the UV Lab software.

PDF 1000 KB
Determination of Total Protein Using the LAMBDA UV/Vis Spectrophotometer: Lowry Method

The Lowry and Biuret methods are standard methods for protein quantification. Ohnishi and Barr1 modified and simplified the Biuret combined reagent for the Lowry procedure and at the same time improved its stability. This application note describes the modified Lowry procedure for quantitative analysis of protein using the LAMBDA 465 and UV Lab software.

PDF 827 KB
Monitoring Fast Chemical Reactions Application Note

Spectrosopic techniques, such as UV-Visible spectrometry, can be deployed to observe the chemical changes taking place during fast reactions. However, in many cases the reaction timescales are too fast to be performed and observed by the simple manual mixing of the reactants in a cuvette. The stopped-flow technique is frequently used for rapid kinetics experiments allowing the reactants to mix effectively and react whilst monitoring the reaction in a low volume cuvette in the UV-Visible instrument. This application note demonstrates how the PerkinElmer LAMBDA 465 Diode Array UV-Visible Spectrophotometer together with the TgK Scientific SFA-20 Rapid Kinetics Accessory allows reaction monitoring on the millisecond timescale.

The Determination of Total Protein Using the LAMBDA UV/Vis Spectrophotometer: Bradford Method

Quantification methods for total protein are among the longest-established fundamental and important experiments of bioscience. UV/Vis Spectrophotometry is widely used for the determination of protein. This application note describes a typical protein method, the Bradford method. Data is rapidly acquired using the LAMBDA™ 465 UV/Vis Spectrophotometer and processed using the UV Lab Software.

PDF 1020 KB
The Quantification of Oligonucleotide Using a LAMBDA 465 UV/Vis Spectrophotometer

Using the LAMBDA 465 UV/Vis Spectrophotometer and UV Lab software, quantification of the oligonucleotide was performed.The range of concentrations was 0.5 - 2.0 µM. Rapid acquirement of spectra and good sensitivity were obtained with the LAMBDA 465.

PDF 958 KB
Water Analysis using LAMBDA Spectrophotometer: Nitrate Nitrogen (NO3-N), Brucine Method

When a water sample containing nitrate ions is treated with brucine in sulfuric acid condition, a yellow compound is created. The quantity of nitrate nitrogen can be determined by measuring the absorbance of the yellow compound at 410 nm. In this application note, the quantitative analysis of Nitrate nitrogen (NO3-N) was performed by Brucine method. Data was rapidly acquired using the LAMBDA™ 465 UV/Vis Spectrophotometer and processed using the UV Lab™ Software.

Water Analysis using LAMBDA: Total Phosphorus (T-P), Ascorbic Acid Method

Principle Total phosphoric compounds in water sample is changed to phosphate (PO4 -) form by oxidation. After treatment with ammonium molybdate • ascorbic acid solution, blue color is created. This color is measured at 880 nm. In this application note, the quantitative analysis of total phosphorus (T-P) was performed by as-corbic acid method. Data are rapidly acquired using LAMBDA™ 465 UV-Vis Spectrophotometer and processed using UV Lab™ Software.



A Guide to General USP Chapters

Quality and consistency of medicines is critical to ensuring their identity, strength, and purity is at a safe level for human consumption.

That's why we rely on strict guidelines – to guarantee medications that make it to the shelves are exactly what they claim to be. This guide helps provide a simple breakdown of USP Chapters and what they contain, so that you can be confident your products are in compliance.

Dissolution Testing Brochure

Pharmaceutical labs consider dissolution testing to be one of the critical steps in the drug development process. It makes an impact at almost every step of the journey, from early product development to late-stage quality control - so it's no surprise that it is a key concern for regulatory bodies. To ensure quality and compliance in your lab, the dissolution testing solution you depend on must provide the most accurate and reproducible results available.

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Meat and Seafood Testing Solutions

Food testing labs like yours are constantly challenged with accurately analyzing samples quickly and efficiently - all while striving to reduce costs due to market forces. Your commitment to ensuring meat and seafood are safe for consumption, as demand increases, is an uphill battle.

Our commitment to you: to provide a range of solutions across multiple technologies, products, and services that meets or exceeds the testing needs of food processors. Our solutions offer more efficiency and increased accuracy and sensitivity for better yields in real time with minimal training.

From instrumentation and software to consumables and reagents to service and support, we are dedicated to providing you with end-to-end solutions that ease your everyday challenges of automation, throughput, service, and time to results.



Palm Oil Analysis. Complete lab solutions from upstream to downstream

Quality control-monitoring and testing are important in ensuring the quality of palm oil. The quality control parameters are used to judge the quality of palm oil products and it can be monitored and tested to ensure that the palm oil is not deliberately or accidentally adulterated.


Regulatory Compliance Certification

Product Certificate - UV Lambda 465

Product Certificate for the UV Lambda 465

PDF 164 KB