PerkinElmer
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LAMBDA 465 UV/Vis Spectrophotometer

Designed specifically for high-end research as well as routine and high-throughput applications, the LAMBDA 465 UV/Vis is the innovative PDA solution that provides maximum reliability – for maximum confidence in your results.

Part Number N4100030
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Detail Information

Ultrafast, high-performance, reliable photodiode array UV Vis

Its PDA technology allows the acquisition of a full spectrum – from 1100 nm to 190 nm – in as little as 20 msec. In addition, the system has 1-nm Optical resolution, allowing it to meet the requirements of a number of pharmacopoeias. With 21 CFR part 11 compliant software, it’s an ideal solution for dissolution, fast kinetics, and other applications where high-speed scanning and high resolution are required – and it’s perfect for method development and sample analysis, too.

For flexibility in sampling along with high resolution and low noise spectrum, a dual light source (tungsten and deuterium) in a see-through configuration is unitized, providing the highest energy throughput possible – an important consideration with accessories that impact energy throughput.

Specifications

21 CFR Part 11 Compatible Yes
Height 23.0 cm
Interface Tungsten-halogen and Deuterium
Maximum Temperature 35 °C
Minimum Temperature 15 °C
Model Name LAMBDA 465
Operating Range 190 - 1100 nm
Portable No
Product Brand Name LAMBDA
Warranty 1 year
Wave Length Range 190 - 1100 nm
Weight 16.0 kg
Width 45.0 cm
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Application Note

Determination of Cholesterol Level in Human Serum - Enzymatic Colorimetric Method

Clinical chemistry uses chemical processes to measure levels of chemical components in the blood. It is very useful for the early diagnostic of disease and for monitoring organ function. The most common specimens used in clinical chemistry are blood and urine and this application note shows the common blood tests and measurable items using UV/Vis spectrophotometers as determined by the enzymatic method.

PDF 830 KB
The Determination of Total Protein Using the LAMBDA UV/Vis Spectrophotometer: Bradford Method

Quantification methods for total protein are among the longest-established fundamental and important experiments of bioscience. UV/Vis Spectrophotometry is widely used for the determination of protein. This application note describes a typical protein method, the Bradford method. Data is rapidly acquired using the LAMBDA™ 465 UV/Vis Spectrophotometer and processed using the UV Lab Software

PDF 1020 KB
The Quantification of Oligonucleotide Using a LAMBDA 465

The efficiency of molecular manipulations involving nucleic acids is heavily dependent on the concentration and purity of the nucleic acid in a sample. The quantification of oligonucleotide is readily accomplished by taking advantage of the absorbance of UV light. Thus, UV light analysis may be used to derive information about the concentration of the sample. This application note describes the quantification of an oligonucleotide using a LAMBDA™ 465 UV/Vis Spectrophotometer.

PDF 958 KB
Water Analysis using LAMBDA Spectrophotometer: Nitrate Nitrogen (NO3-N), Brucine Method

When a water sample containing nitrate ions is treated with brucine in sulfuric acid condition, a yellow compound is created. The quantity of nitrate nitrogen can be determined by measuring the absorbance of the yellow compound at 410 nm. In this application note, the quantitative analysis of Nitrate nitrogen (NO3-N) was performed by Brucine method. Data was rapidly acquired using the LAMBDA™ 465 UV/Vis Spectrophotometer and processed using the UV Lab™ Software.

PDF 1 MB
Water Analysis using LAMBDA: Total Phosphorus (T-P), Ascorbic Acid Method

Principle Total phosphoric compounds in water sample is changed to phosphate (PO4 -) form by oxidation. After treatment with ammonium molybdate • ascorbic acid solution, blue color is created. This color is measured at 880 nm. In this application note, the quantitative analysis of total phosphorus (T-P) was performed by as-corbic acid method. Data are rapidly acquired using LAMBDA™ 465 UV-Vis Spectrophotometer and processed using UV Lab™ Software.

PDF 1 MB

Regulatory Compliance Certification