Designed specifically for high-end research as well as routine and high-throughput applications, the LAMBDA™ 465 UV/Vis is the innovative PDA solution that provides maximum reliability – for maximum confidence in your results.
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Its PDA technology allows the acquisition of a full spectrum – from 1100 nm to 190 nm – in as little as 20 msec. In addition, the system has 1-nm Optical resolution, allowing it to meet the requirements of a number of pharmacopoeias. With 21 CFR part 11 compliant software, it’s an ideal solution for dissolution, fast kinetics, and other applications where high-speed scanning and high resolution are required – and it’s perfect for method development and sample analysis, too.
For flexibility in sampling along with high resolution and low noise spectrum, a dual light source (tungsten and deuterium) in a see-through configuration is unitized, providing the highest energy throughput possible – an important consideration with accessories that impact energy throughput.
|21 CFR Part 11 Compatible||Yes|
|Interface||Tungsten-halogen and Deuterium|
|Maximum Temperature||35 °C|
|Minimum Temperature||15 °C|
|Model Name||LAMBDA 465|
|Operating Range||190 - 1100 nm|
|Product Brand Name||LAMBDA|
|Wave Length Range||190 - 1100 nm|
Clinical chemistry uses chemical processes to measure levels of chemical components in the blood. It is very useful for the early diagnostic of disease and for monitoring organ function. The most common specimens used in clinical chemistry are blood and urine and this application note shows the common blood tests and measurable items using UV/Vis spectrophotometers as determined by the enzymatic method.
The level of Hydroxymethylfurfural (HMF) present is a key indicator as to the quality of honey. In this application note, the HMF content has been determined using the LAMBDA™ 465 UV/Vis Spectrophotometer and the Equation Calculation mode in the UV Lab™ software.
In this application note, the amount of sugar or carbohydrate in a soft drink was determined using a colorimetric method. The rapid measurement of the PDA (Photodiode Array) UV/Vis Spectrophotometer allows for the collection of accurate data from the time-dependent reaction. The calibration curve was automatically calculated using the Quantification mode of the UV Lab™ software.
The Lowry and Biuret methods are standard methods for protein quantification. Ohnishi and Barr1 modified and simplified the Biuret combined reagent for the Lowry procedure and at the same time improved its stability. This application note describes the modified Lowry procedure for quantitative analysis of protein using the LAMBDA 465 and UV Lab software.
Spectrosopic techniques, such as UV-Visible spectrometry, can be deployed to observe the chemical changes taking place during fast reactions. However, in many cases the reaction timescales are too fast to be performed and observed by the simple manual mixing of the reactants in a cuvette. The stopped-flow technique is frequently used for rapid kinetics experiments allowing the reactants to mix effectively and react whilst monitoring the reaction in a low volume cuvette in the UV-Visible instrument. This application note demonstrates how the PerkinElmer LAMBDA 465 Diode Array UV-Visible Spectrophotometer together with the TgK Scientific SFA-20 Rapid Kinetics Accessory allows reaction monitoring on the millisecond timescale.
Quantification methods for total protein are among the longest-established fundamental and important experiments of bioscience. UV/Vis Spectrophotometry is widely used for the determination of protein. This application note describes a typical protein method, the Bradford method. Data is rapidly acquired using the LAMBDA™ 465 UV/Vis Spectrophotometer and processed using the UV Lab Software.
Using the LAMBDA 465 UV/Vis Spectrophotometer and UV Lab software, quantification of the oligonucleotide was performed.The range of concentrations was 0.5 - 2.0 µM. Rapid acquirement of spectra and good sensitivity were obtained with the LAMBDA 465.
When a water sample containing nitrate ions is treated with brucine in sulfuric acid condition, a yellow compound is created. The quantity of nitrate nitrogen can be determined by measuring the absorbance of the yellow compound at 410 nm. In this application note, the quantitative analysis of Nitrate nitrogen (NO3-N) was performed by Brucine method. Data was rapidly acquired using the LAMBDA™ 465 UV/Vis Spectrophotometer and processed using the UV Lab™ Software.
Principle Total phosphoric compounds in water sample is changed to phosphate (PO4 -) form by oxidation. After treatment with ammonium molybdate • ascorbic acid solution, blue color is created. This color is measured at 880 nm. In this application note, the quantitative analysis of total phosphorus (T-P) was performed by as-corbic acid method. Data are rapidly acquired using LAMBDA™ 465 UV-Vis Spectrophotometer and processed using UV Lab™ Software.
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