PerkinElmer
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LAMBDA 465 UV/Vis Spectrophotometer

Designed specifically for high-end research as well as routine and high-throughput applications, the LAMBDA 465 UV/Vis is the innovative PDA solution that provides maximum reliability – for maximum confidence in your results.

Part Number N4100030
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Overview

Ultrafast, high-performance, reliable photodiode array UV Vis

Its PDA technology allows the acquisition of a full spectrum – from 1100 nm to 190 nm – in as little as 20 msec. In addition, the system has 1-nm Optical resolution, allowing it to meet the requirements of a number of pharmacopoeias. With 21 CFR part 11 compliant software, it’s an ideal solution for dissolution, fast kinetics, and other applications where high-speed scanning and high resolution are required – and it’s perfect for method development and sample analysis, too.

For flexibility in sampling along with high resolution and low noise spectrum, a dual light source (tungsten and deuterium) in a see-through configuration is unitized, providing the highest energy throughput possible – an important consideration with accessories that impact energy throughput.

Specifications

21 CFR Part 11 Compatible Yes
Height 23.0 cm
Interface Tungsten-halogen and Deuterium
Maximum Temperature 35 °C
Minimum Temperature 15 °C
Model Name LAMBDA 465
Operating Range 190 - 1100 nm
Portable No
Product Brand Name LAMBDA
Warranty 1 year
Wave Length Range 190 - 1100 nm
Weight 16.0 kg
Width 45.0 cm
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Application Note

Determination of Cholesterol Level in Human Serum - Enzymatic Colorimetric Method

Clinical chemistry uses chemical processes to measure levels of chemical components in the blood. It is very useful for the early diagnostic of disease and for monitoring organ function. The most common specimens used in clinical chemistry are blood and urine and this application note shows the common blood tests and measurable items using UV/Vis spectrophotometers as determined by the enzymatic method.

PDF 830 KB
The Determination of Total Protein Using the LAMBDA UV/Vis Spectrophotometer: Bradford Method

Quantification methods for total protein are among the longest-established fundamental and important experiments of bioscience. UV/Vis Spectrophotometry is widely used for the determination of protein. This application note describes a typical protein method, the Bradford method. Data is rapidly acquired using the LAMBDA™ 465 UV/Vis Spectrophotometer and processed using the UV Lab Software.

PDF 1020 KB
The Quantification of Oligonucleotide Using a LAMBDA 465 UV/Vis Spectrophotometer

Using the LAMBDA 465 UV/Vis Spectrophotometer and UV Lab software, quantification of the oligonucleotide was performed.The range of concentrations was 0.5 - 2.0 µM. Rapid acquirement of spectra and good sensitivity were obtained with the LAMBDA 465.

PDF 958 KB
Water Analysis using LAMBDA Spectrophotometer: Nitrate Nitrogen (NO3-N), Brucine Method

When a water sample containing nitrate ions is treated with brucine in sulfuric acid condition, a yellow compound is created. The quantity of nitrate nitrogen can be determined by measuring the absorbance of the yellow compound at 410 nm. In this application note, the quantitative analysis of Nitrate nitrogen (NO3-N) was performed by Brucine method. Data was rapidly acquired using the LAMBDA™ 465 UV/Vis Spectrophotometer and processed using the UV Lab™ Software.

PDF 1 MB
Water Analysis using LAMBDA: Total Phosphorus (T-P), Ascorbic Acid Method

Principle Total phosphoric compounds in water sample is changed to phosphate (PO4 -) form by oxidation. After treatment with ammonium molybdate • ascorbic acid solution, blue color is created. This color is measured at 880 nm. In this application note, the quantitative analysis of total phosphorus (T-P) was performed by as-corbic acid method. Data are rapidly acquired using LAMBDA™ 465 UV-Vis Spectrophotometer and processed using UV Lab™ Software.

PDF 1 MB

Brochure

Dissolution Testing Brochure

Pharmaceutical labs consider dissolution testing to be one of the critical steps in the drug development process. It makes an impact at almost every step of the journey, from early product development to late-stage quality control - so it's no surprise that it is a key concern for regulatory bodies. To ensure quality and compliance in your lab, the dissolution testing solution you depend on must provide the most accurate and reproducible results available.

PDF 858 KB

Regulatory Compliance Certification