PerkinElmer
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MaxSignal® Colistin ELISA Test Kit

The MaxSignal® Colistin ELISA Kit is a competitive enzyme immunoassay for the quantitative analysis of colistin in beef.

Part Number FOOD-1095-01A
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Overview

Rapid ELISA for the Screening of Colistin

The MaxSignal® Colistin ELISA Test Kit is an effective tool for the screening of colistin in a variety of sample types. This kit combines a unique and proprietary colistin extraction method along with stable standards to deliver dependable, accurate, rapid, cost effective, and easy colistin analysis.

The MaxSignal Colistin ELISA Kit is based on a competitive colorimetric ELISA assay. A colistin antibody has been coated in the plate wells. During the analysis, sample is added along with the primary colistin antibody. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Colistin, also known as polymyxin E, is a cationic lipopeptide antibiotic isolated in 1950 from Bacillus polymyxa subsp. colistinus. It is most notable for its activity toward the multidrug-resistant organism Pseudomonas aeruginosa and low rates of resistance. Colistin is a mixture of cyclic polypeptides colistin A and B. Colistin is effective against most Gram-negative bacilli. Food of animal origin is also tested for colistin residues because of the possible risks for human health. It is restricted in the EU and Japan.

This kit is manufactured to the international quality standard ISO 9001.

References that Cite the Use of the MaxSignal Colistin ELISA Test Kit:

  • Azzopardia, E. A., Fergusona, E. L. and Thomasa, D. W. (2015) Development and Validation of an In Vitro Pharmacokinetic/Pharmacodynamic Model To Test the Antibacterial Efficacy of Antibiotic Polymer Conjugates. Antimicrob. Agents Chemother. 59:4, 1837-1843.
  • Detection Limit:
    • Beef 5 ng/g or ppb

Specifications

Quantity in a Package Amount 96.0 Tests
Research Areas Food & Agriculture
Sample Type Beef
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Brochure

Meat and Seafood Testing Solutions

Food testing labs like yours are constantly challenged with accurately analyzing samples quickly and efficiently - all while striving to reduce costs due to market forces. Your commitment to ensuring meat and seafood are safe for consumption, as demand increases, is an uphill battle.

Our commitment to you: to provide a range of solutions across multiple technologies, products, and services that meets or exceeds the testing needs of food processors. Our solutions offer more efficiency and increased accuracy and sensitivity for better yields in real time with minimal training.

From instrumentation and software to consumables and reagents to service and support, we are dedicated to providing you with end-to-end solutions that ease your everyday challenges of automation, throughput, service, and time to results.

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