PerkinElmer

Application Note

Resurgence in Fluorinated Chiral Polysaccharide Phases for Supercritical Fluid Chromatography

Introduction

Chiral supercritical fluid chromatography (SFC) is the primary tool for the separation of enantiomers in support of drug discovery efforts. SFC uses a simple mobile phase system consisting of non-toxic CO2 and an organic modifier, typically an alcohol. The lower toxicity of solvents and reduced overall waste generation of SFC make this a greener alternative to normal phase HPLC and is thus ideal for preparative scale applications including chiral separations. Building upon existing research, several new chiral stationary phases have been created to explore fluorophilic retention mechanisms towards enantioseparation of fluorinated compounds.