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Application Note

Determination of Antibiotics and Veterinary Drugs in Milk by QSight LC/MS/MS

Introduction

In this study we show two methods for the analysis of over 30 antibiotics and veterinary drugs in milk regulated by the Food Safety and Standards Authority of India (FSSAI). For polar analyte analysis, such as aminoglycoside antibiotics (apramycin, spectinomycin, and neomycin) in milk, a mixed mode LC method was used to improve analyte retention and a trichloroacetic acid - acetonitrile extraction method was used to enhance analyte extraction efficiency. For less polar analytes, a reversed phase UHPLC method was used for analyte separation and an acidified acetonitrile extraction was applied for sample preparation. The methodology can be extended to analysis of similar analytes in milk and milk products regulated by other regulatory bodies.