Application Note

Determination of Sulfonamides in milk by Ultra-Performance Liquid Chromatography coupled with Tandem Mass Spectrometry (QSight™)


Antibiotics are used in dairy cattle for different treatments, but it leads to accumulation of these residues in milk. Presence of such residues in human food can be responsible for allergic reactions, toxic effects and develops resistant strains of bacteria. To protect consumers, regulatory agencies in the European Union published several official documents to regulate the control of veterinary drugs. Council Directive 96/23/EC establishes the veterinary residue control in food producing animals. In India, as per FSSAI (gazette notification dated 20th July 2018) and EIC (RMP for Aquaculture, Egg, Honey, Milk Poultry etc.) it is a mandate to do the analysis of antibiotics. EIC and FSSAI both have maximum residue limits (MRLs) for various antibiotic residues for food matrices from animals. It is essential to test milk for residues of sulfonamides considering safety and prevent exposure of consumers to veterinary drugs. In this work, we present a fast and sensitive LC-MS/MS method for the quantitative analysis of sulfonamides antibiotics in milk.