Nitrite, occurs naturally in the environment as an intermediate of the nitrogen cycle through microbial decomposition of organic matter. However, nitrite contains nitrogen in a reasonably unstable oxidation state and readily oxidizes to nitrate. As a result, nitrite is present at considerably lower concentrations in ground and surface waters than nitrate1. Higher concentrations of nitrite are indicative of pollution by industrial wastewater or agricultural run-off. In this application, the quantitative analysis of nitrite-nitrogen was performed using the LAMBDA™ 265 UV-Vis spectrophotometer and CHEMetrics nitrite test kit.