Immunoassays in Food Testing
As a leader in the food and feed safety testing industry, PerkinElmer develops, manufactures, and markets a wide range of test kits for the detection of antibiotics, hormones, veterinary drug residues, natural toxins, pathogens, and a variety of industrial contaminants found within the global food chain. Our goal is to provide food producers, processors, and government agencies with high quality test kits enabling compliance with global and local regulations to produce safe food for their customers. Highlights of our food and feed safety product line include:
With our quality management system certified to ISO 9001 standards, we strive to deliver high quality products and services in conjunction with exceptional customer service and technical support.
Immunoassays in Food Technologies
An ELISA or Enzyme Linked Immuno-Sorbent Assay allows for the rapid screening and quantitation of analytes against a variety of materials. PerkinElmer offers a wide range of ELISA kits to measure the safety of dairy, meat, poultry, seafood, grain, feed, and honey.
ELISAs involve at least one antibody with specificity for a particular analyte or antigen and consist of the following basic protocol:
PerkinElmer's Solus Pathogen Detection System provides highly efficient assays for the detection of Salmonella, Listeria or E. coli O157 in food and environmental samples. Each system comprises a specifically designed immunoassay kit, dedicated selective enrichment media and automated liquid handling. Our pathogen detection system is robust, reliable and all assays are validated by external independent laboratories to AFNOR 16140 and/or certified by the AOAC Performance Tested Method program.
When automated, our immunoassays deliver the capacity to maximise the use of available laboratory resources and optimise lab productivity.
The lateral flow immunoassay is a convenient, rapid, strip-based technology for the detection of small molecules, including antibiotics and toxins, by harnessing the ability of purified antibodies to bind to one another in a highly specific and concerted movement along a membrane. PerkinElmer offers a range of lateral flow test strip products that can give farmers, factory workers, and scientists in numerous food industries the solutions they need to qualitatively and quantitatively confirm that the material they produce is safe for the consumer or ready for further downstream processing.
Lateral flow test strips are designed to detect specific target antibiotics or analytes in a variety of sample types with high specificity and accuracy in accordance with various regulatory agencies such as the USDA, European Union and CODEX maximum residual limits (MRL). The testing procedure can be completed in minutes with minimal opportunities for error, as PerkinElmer strives to make the end-user testing experience as friendly, efficient, and accurate as possible. Results can be interpreted visually or with a PerkinElmer Lateral Flow Reader.
Histamine testing is a control strategy used by seafood processors in Hazard Analysis and Critical Control Points(HACCP). A strong need exists for a histamine detection method that couples simple extraction with high-accuracy quantification. The MaxSignal® Histamine Enzymatic Assay Kit is one such robust enzyme-based assay, using a simple aqueous extraction with high recovery in a variety of fish samples such as fresh/frozen tuna, canned tuna, pouched tuna, and mahi-mahi.
Ractopamine has been banned for use as a livestock growth promoter in many regions and countries including Europe and China. To prevent ractopamine residues from entering the food chain, both producers and government surveillance agencies need technologies and methods that can provide rapid, accurate and reliable detection at specific sensitivities. MaxSignal® Ractopamine ELISA Kit enables government agencies, food manufacturers, as well as quality assurance organizations, to detect ractopamine as low as 0.1 ng/g or 0.1 ppb in a variety of sample types. In the current study, an improved sample preparation protocol was developed and validated for swine feed.
The use of nitrofurans, a class of broad-spectrum antibiotics widely used in the aquaculture industry and their metabolites, has been regulated by everal countries and organizations across the world for their ahrmful effects on human health. ELISA assays are widely used for the detection of nitrofuran metabolites and chloramphenicol for regulatory conformance owing to the high sensitivity, selectivity and ease of use of the method. In this study, we demonstrate the accuracy and precision (CCß validation study) of the simultaneous 5-in-1 sample extraction method by manual ELISA and DS2 automation methods. Sensitivityof the assay kits (LOD) was also demonstrated using manual and DS2 method. Finally, sample variability testing was performed to characterize the effects of matrix from various shrimp sources.
Solus Pathogen Detection System offer food testing laboratories a highly efficient method for the detection of Salmonella, Listeria, and E. coli O157 in food and environmental samples.
Solus Salmonella™ offers food testing laboratories an easy to use, cost effective and efficient method for the detection of Salmonella in food and environmental samples.
In a fast-paced food testing environment, processing samples quickly and efficiently is critical, enabling production facilities to take immediate action when necessary. As sample volumes fluctuate, your lab must have the flexibility to cope with varying demands, maximizing throughput with optimal use of resources. That’s the kind of capability our Solus One Salmonella pathogen detection system can provide. Solus One Salmonella is a highly efficient assay for the next day detection of Salmonella in food and environmental samples. The immunoassay has been developed alongside dedicated selective enrichment media and can be automated using the Dynex DS2 for a complete pathogen detection system.
SOLUS ONE ListeriaRapid testing and enhanced throughput for environmental samples
Solus Listeria™ provides laboratories with an easy to use, cost effective and efficient method for the detection of Listeria in food and environmental samples.
Solus E. coli O157™ offers food testing laboratories an easy to use, cost effective and efficient method for the detection of E. coli O157™ in food and environmental samples.
ALS Rotherham test over half a million samples each year. Samples include raw and cookedmeats and fish, dairy, chocolate and a number of ready-to-eat products. The laboratory offersa mixture of conventional culture methods and automated immunoassays to process over2000 samples per day.
The AuroFlow™ PR1ME™ BT Combo MRL Assay is able to detect 14 beta-lactam antibiotics under the European MRL levels, as well as the metabolites of ceftiofur and cephaprin below MRL levels. The assay can detect all 3 regulated tetracycline antibiotics under the European MRL.The validation results indicate that the AuroFlow™ PR1ME™ BT Combo MRL Assay is appropriate for use as a screening test for beta-lactams and tetracyclines in raw commingled cow’s milk.
Histamine poisoning is the second leading cause of food-borne illness in the United States, accounting for nearly one in fifty of the total cases of food-borne illness in the US. The expensive, time-consuming, and labor-intensive nature of the available detection methods for histamine dramatically limit the capacity to test large numbers of samples. The HistaStrip™ Test Kit offers a convenient solution for quick, quantitative, field-based screening of seafood samples.