Columns - Liquid Chromatography

ChromegaChiral^™ CCO F4 T3 (tris(4-Fluoro-3-(trifluoromethylphenyl carbamate) cellulose) incorporates fluoro groups into its structure, and can be used in SFC or HPLC. The addition of a fluorine atom into a phenyl cellulose structure can be useful in promoting fluorophilic retention mechanism which can provide improved retention for fluorinated compounds. A fluorophilic retention mechanism can be particular useful in medicinal chemistry and drug discovery, where more than a third of newly approved small molecule drugs contain fluorine.

A polysaccharide coated chiral stationary phase and columns which are produced using a unique production process of coating the proven chiral selector, tris-(3,5-dimethylphenylcarbamate) cellulose on high purity, high performance silica. ChromegaChiral^™ CCO columns are similar in selectivity to CHIRALCEL^® OD.

Sterically protected ligand provides greater stability at low pH where most peptide separations are performed.

Epic^™ Polar is a high density C18 packing specifically engineered for the retention of polar analytes and allows full interaction with the bonded hydrocarbon phase, even in 100% aqueous mobile phases. Under these highly aqueous conditions, our novel proprietary bonding chemistry allows the bonded chains to remain fully extended in the mobile phase. Epic Polar can retain highly water-soluble compounds such as small organic acids, water-soluble vitamins, purines and pyrimidines, cathecholamines and other polar compounds.

ChromegaChiral^™ CCO F4 is a tris(4-Fluoro 3-methylphenylcarbamate) cellulose phase which can be used in SFC or HPLC. The addition of a fluorine atom into a phenyl carbamate cellulose structure is useful in promoting a fluorophilic retention mechanism which provides improved retention for fluorinated compounds. A fluorophilic retention mechanism is particularly useful in medicinal chemistry and drug discovery, where more than a third of newly approved small molecule drugs contain fluorine.

Epic^™ Silica is the backbone for the Epic bonded product range and is a 120 Angstrom high purity metal free synthetic silica. The Epic Silica is the only Epic column designed for use in normal phase chromatography. The Epic Silica Prep columns are very effective in preparative chromatography as normal phase organic solvents are more easily removed than reversed phase solvents.

Epic^™ Phenyl-Hexyl employs a 6-carbon (hexyl) linked phenyl phase bonded to high purity silica, where the hexyl alkyl chain delivers unique selectivity and increased hydrolytic stability when compared to propyl-linked phenyl phase chemistry. This retentive phase provides different selectivity to straight chain hydrocarbon phases like C6, C8 or C18, and it is especially useful for aromatic analytes, complex samples and polar pharmaceutical compounds. The Epic high-density bonding technology delivers to the chromatographer superior performance, durability, and enhanced lot-to-lot reproducibility. Epic Phenyl-Hexyl offers the chromatographer a truly superior phenyl-based interaction for enhanced chromatographic performance.

Epic^™ Biphenyl is a truly unique stationary phase with properties significantly different than ODS phases. This unique character results from bonded biphenyl groups, covalently attached to high purity silica, imparting a π-π electron interaction which produces an enhanced retention for many compounds particularly those that contain polarizable electrons. Many classes of compounds contain polarizable electrons including halogenated compounds, aromatics, nitro aromatics and conjugated systems.

Reduced activity C18 phase recommended for the fast, reversed-phase separation of basic compounds/pharmaceuticals.

High purity, general purpose Octadecyl (C18) phase with wide selectivity. Recommended for the reverse phase separation of acidic, basic, or neutral compounds.

The Epic^™ PFP LB (low bleed) is a pentafluorophenyl that has been baseline stabilized and is ready for high performance separations. The Epic PFP LB is a truly unique stationary phase with properties significantly different than C18 phases. This unique characteristic results from bonded pentafluorophenyl groups imparting a π-π electron interaction which produces an enhanced retention for many compounds, such as natural products, halogenated compounds, aromatics, conjugated compounds and trace impurities in complex matrices. Many of these high-performance separations were not possible with existing PFP columns especially in the area of trace impurities where baseline bleed levels were unacceptable. Epic PFP LB columns have been stabilized to provide low column bleed, increased lifetimes, better pH stability, and superior LC-MS performance.

ChromegaChiral^™ CCX is a modified amylose coated on high purity, high performance spherical silica particles. The chemical modification includes the combination of chemical bonding groups of methylbenzylcarbamate and 3,5-dimethylphenylcarbamate attached to amylose. This combination of bonded groups stabilizes the solubility of coated phase, making for a durable phase similar to other widely used coated phases. The use of amylose modified with phenyl groups moiety provides for the separation for many previously unresolved/poorly resolved chiral mixtures. This chemical modification provides for unique separation behaviour.

Epic^™ Polar is a high density C18 packing specifically engineered for the retention of polar analytes and allows full interaction with the bonded hydrocarbon phase, even in 100% aqueous mobile phases. Under these highly aqueous conditions, our novel proprietary bonding chemistry allows the bonded chains to remain fully extended in the mobile phase. Epic Polar can retain highly water-soluble compounds such as small organic acids, water-soluble vitamins, purines and pyrimidines, cathecholamines and other polar compounds.

Epic^™ C18 is a highly inert phase due to its superior base deactivation. As a result of the high-density bonding levels (> 4 µmol/m2), Epic C18 demonstrates superior peak shapes for the most demanding applications. This phase provides exceptional peak shape and selectivity over a wide range of compounds and pH and is the workhorse HPLC and UHPLC phase for RP small molecule analysis. It can be used with basic, neutral and acidic analytes.

ChromegaChiral^™ CCJ (cellulose tris(4-methylbenzoate)) is a new product for high resolution chiral separations based on a new halogenated carbohydrate based chiral stationary phase. Similar in selectivity to CHIRALCEL^® OJ-H.

Epic^™ C18 MS is specifically engineered for the demands of LC-MS and is a product of high bonding density, allowing maximum stationary phase interaction and providing a platform for flexible mobile phase compositions. It provides superior retention even at high organic levels. Many LC-MS applications rely on high level of organic modifier in the mobile phase, particularly for high performance trace analysis requiring high sensitivity and low-level detection; Epic C18 MS is ideally suited for these mobile phase conditions. This phase is compatible with all LC-MS mobile phases and buffer systems.

Reduced activity C18 phase recommended for the fast, reversed-phase separation of basic compounds/pharmaceuticals.

Less retentive, high purity Octyl (C8) phase for reverse phase separations.

ChromegaChiral^™ CCO F2 is a tris(2-Fluoro 5-methylphenylcarbamate) cellulose phase which can be used in SFC or HPLC. The addition of a fluorine atom into a phenyl carbamate cellulose structure can be useful in promoting a fluorophilic retention mechanism which can provide improved retention for fluorinated compounds. A fluorophilic retention mechanism can be particularly useful in medicinal chemistry and drug discovery, where more than a third of newly approved small molecule drugs contain fluorine.

Epic^™ C18 MS is specifically engineered for the demands of LC-MS and is a product of high bonding density, allowing maximum stationary phase interaction and providing a platform for flexible mobile phase compositions. It provides superior retention even at high organic levels. Many LC-MS applications rely on high level of organic modifier in the mobile phase, particularly for high performance trace analysis requiring high sensitivity and low-level detection; Epic C18 MS is ideally suited for these mobile phase conditions. This phase is compatible with all LC-MS mobile phases and buffer systems.

ChromegaChiral^™ CCU is a modified amylose coated on high purity, high performance spherical silica particles. The chemical modification includes the combination of chemical bonding groups of methylbenzylcarbamate and 3-chloro-4 methylphenylcarbamate attached to amylose. This combination of bonded groups stabilizes the solubility of coated phase making for a durable phase similar to other widely used coated phases. The use of amylose modified with phenyl groups provides for the separation for many previously unresolved/poorly resolved chiral mixtures.

Epic^™ Amine HD (high density) is a polymeric amino phase bonded to silica. Applications include HILIC, weak acids, and sugars. The Epic bonding technology produces columns of superior performance and durability. These performance characteristics result from the high bonding density found in the Epic bonding process.

The Epic^™ Phenyl phase is π-basic (electron donating) and is similar in overall retention to alkyl phases. The alternate selectivity exhibited by phenyl phases is explained by the π-π interactions available through the phenyl ring. Compounds that exhibit this alternate selectivity on the Epic Phenyl phase include antibiotics such as tetracycline, moderate bases such as anesthetics, some acidic compounds such as quinoline antibiotics and nucleosides. The Epic high-density bonding technology delivers superior performance, durability and enhanced lot-to-lot reproducibility. Epic Phenyl offers a truly superior phenyl-based interaction for enhanced chromatographic performance.

Epic^™ Silica is the backbone for the Epic bonded product range and is a 120 Angstrom high purity metal free synthetic silica. The Epic Silica is the only Epic column designed for use in normal phase chromatography. The Epic Silica Prep columns are very effective in preparative chromatography as normal phase organic solvents are more easily removed than reversed phase solvents.