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  • Flexar UHPLC

    Flexar UHPLC

    For demanding applications when you need to collect more information in less time, our Flexar UHPLC is the perfect solution. This UHPLC is designed for a high productivity environment and delivers high sensitivity and resolution, exceptional flow accuracy and precision and faster results.
  • Flexar LC

    Flexar LC

    Meet the HPLC system you can count on day after day. For routine analyses, it simply streamlines your processes with easy, trouble-free operation while delivering reliable results every time.
  • NexSAR HPLC Stack

    NexSAR Speciation Analysis Ready HPLC System

    The NexSAR HPLC, is a next-generation Speciation Analysis Ready system engineered with a completely inert and metal-free fluid path, enabling laboratories to meet low chromatographic background requirements on the most challenging speciation applications. Plus, it is fully customizable, with the flexibility to select only the parts that you need for your application(s).

    This system is part of the NexSAR HPLC-ICP-MS Speciation Solution, which couples the NexSAR HPLC with our revolutionary NexION® ICP-MS and is seamlessly integrated using the proven Clarity software.

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1-14 of 14 Business Insights

  • Application Note

    UHPLC-PDA Analysis of Curcuma Using a Quasar SPP Column

    Turmeric is the powdered dry rhizome of the plant Curcuma longa L., and is used as a coloring agent to give a yellow-orange color to food dishes.

    It has been found to be a rich source of phenolic compounds, namely, three curcuminoids: curcumin (C), demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC). Research to date suggests that turmeric, in addition to having an immunomodulatory role, is also of use in preventing oxidative stress that can lead to inflammation, cancer, and arthritis. This application note describes the sample preparation and analytical method for the chromatographic separation and quantitative monitoring of the three curcuminoids in commercially available turmeric spices and roots by UHPLC, using photodiode array (PDA) detection.

  • Application Brief

    HPLC Analysis of the Semi-Synthetic Antibiotic Azithromycin Using a Quasar C18 Column

    Azithromycin, discovered in 1980, is a semi-synthetic macrolide antibiotic derived from erythromycin. The drug is primarily used to treat various bacterial infections including respiratory infections, acute bacterial sinusitis, skin infections, Lyme disease, and some sexually transmitted infections. The mode of action of azithromycin is by inhibiting bacterial protein synthesis through binding to the 50S subunit of the bacterial ribosome, thereby inhibiting translation of mRNA. The USP monograph for the analysis of azithromycin states the use of a high pH mobile phase (pH 11), which requires a specific and expensive column. The method also uses amperometric electrochemical detection, which is often unavailable in many laboratories. HPLC with a UV or PDA detector provides a good alternative as it is available in most laboratories. This application brief describes the use of a Quasar C18 column in the analysis of azithromycin.

  • Application Brief

    HPLC Analysis of Fluconazole Using Quasar C18 Columns in Accordance with USP Application Brief

    Fluconazole is an azole anti-fungal medication used primarily in the treatment of a wide range of fungal infections. Examples include thrush, urinary tract infections and the prevention of fungal infections in immunocompromised patients undergoing chemotherapy. The mechanism of action can be described as fungistatic, the growth and replication of fungi is inhibited by fluconazole rather than directly attacked. This process is achieved by inhibiting enzymes which are important in the production of fungal membranes, allowing the immune system ample opportunity to target and degrade the pathogen. Fluconazole has generic status and is thus globally produced and prescribed. It is on the World Health Organization’s (WHO) list of essential medicines, which serves as a model of the safest and most effective medications needed by a health system. This application brief describes the use of a Quasar C18, on both porous silica and SPP silica phases, for the analysis of fluconazole in accordance with the official USP monograph.

  • Application Brief

    HPLC Analysis of Ibuprofen Using Quasar C18 Columns in Accordance with the United States Pharmacopeia

    Ibuprofen belongs to the class of compounds known as Nonsteroidal Anti-inflammatory Drugs (NSAIDS) which are used in the treatment of pain, fever and various types of inflammation. NSAIDS work by inhibiting the synthesis of a wide range of prostaglandins produced as a response to injury, strain or illness. Since its introduction in 1969, its tolerability and effectiveness have made it one of the most popularly prescribed/taken medications in the world, with over 24 million prescriptions in the U.S. alone. Ibuprofen has generic status with many manufacturers, making it a very commercially important compound. Projections have estimated a global market value of ibuprofen to be 294.4 million USD in 2020, with an estimated value of 447.6 million USD by 2026. This application brief describes the use of a Quasar C18, on both porous silica and SPP silica phases for the analysis of ibuprofen in accordance with the official USP monograph, specifically, the chromatographic purity section.

  • Application Brief

    HPLC Analysis of Betamethasone Dipropionate Using a Quasar C18 Column in Accordance with the United States Pharmacopeia

    Glucocorticoid steroids work by suppressing various aspects of the human immune system in conditions where hyperactivity can cause poor health through allergies, inflammation and autoimmune dysfunction. Betamethasone dipropionate belongs to this class of steroids. It is used for its high potency as an anti-inflammatory and immunosuppressant in the treatment of diseases such as eczema, dermatitis and psoriasis. Betamethasone dipropionate is classified as a ‘super-potent’ steroid in the treatment of psoriasis in comparison with betamethasone valerate (another common analogue of betamethasone) which is rated as upper mid-strength. This application brief describes the use of a Quasar C18 column for the analysis of betamethasone dipropionate in accordance with the official USP monograph.

  • Application Note

    High Speed Chromatographic Analysis of 16 Cannabinoids by HPLC-PDA

    Current trends for the analysis of the cannabinoid content in cannabis flower extracts/concentrates and commercially available fortified foods depend on liquid chromatography for potency testing and/or ensuring the label-claim accuracy in product content descriptions. This application note describes a fast and robust chromatographic method for the separation and quantitation of 16 cannabinoids commonly analyzed in cannabis-centric labs, using the PerkinElmer Flexar HPLC system with a PDA detector. To further validate the performance of this method for the industry, The Emerald Test Proficiency Test (PT) for Potency was conducted. The Emerald Test™ is an Inter-Laboratory Comparison and Proficiency Test (ILC/PT) program for cannabis testing labs. The results from the PT inter-laboratory samples passed; therefore, the method meets inter-laboratory reproducibility and accuracy.

  • Application Brief

    HPLC Analysis of Paracetamol Using a Quasar C8 Column Based on the USP Application Brief

    Paracetamol, also known as acetaminophen, is an analgesic/antipyretic drug, that reduces fever and pain and is ubiquitous in treating these symptoms. Providing relief for these common symptoms combined with the fact that paracetamol is very well tolerated (in comparison with other analgesics such as aspirin) go towards explaining why it is so widely used. Paracetamol has generic status with many manufacturers making it a commercially important compound. Despite being so widely used the mechanism of action of paracetamol is not fully known. It has been shown to interact with same enzymes as non-steroidal anti-inflammatory drugs, but exclusively in the central nervous system as oppose to the periphery. This application brief describes the use of a Quasar C8 for the analysis of paracetamol adapted from the official USP monograph.

  • Application Brief

    Rapid and Robust HPLC Analysis of Indoxyl Sulfate Using Quasar C18 Column

    Indoxyl sulfate is one of the most extensively studied solutes that accumulates in the plasma when the kidneys fail. Originally called "indican", it was first isolated by Obermayer and Popper in 1911. High concentrations of indoxyl sulfate in blood plasma are known to be associated with the development and progression of a number of pathological conditions including chronic kidney disease and vascular disease. The scientific literature documents the use of older generation C18 columns, using type A silicas. This application brief will illustrate the application of a new generation C18 column, based on type B silica, for the analysis of indoxyl sulfate, Figure 1, as part of a research study to measure the total levels in a simulated blood serum environment.

  • Flyer

    NexSAR HPLC-ICP-MS Speciation Solution

    Speciation studies can provide comprehensive information regarding ion mobility, bioavailability, and toxicity. Consequently, many modern labs are expanding their analytical portfolio to include HPLC-ICP-MS. For such studies, it is advantageous to have an intuitive, user-friendly system that meets the user requirements for automation, ultra-low baseline, powerful integration, and flexible data export. And no one offers a more integrated, versatile, and inert speciation solution than PerkinElmer - accelerate your speciation analysis with the NexSAR™ HPLC-ICP-MS Speciation Solution.

  • Application Brief

    HPLC Analysis of Omeprazole Using a Quasar C8 Column in Accordance with the United States Pharmacopeia

    Omeprazole belongs to a class of drugs known as Proton Pump Inhibitors (PPI’s). It is used in the treatment of conditions relating to overactive stomach acid such as gastroesophageal reflux disease, peptic ulcers and Zollinger-Ellison syndrome. The World Health Organization regards omeprazole among its ‘List of Essential Medicines’ which outlines the most effective and safest medicines required by a countries healthcare system to meet its needs. Omeprazole is a very commercially important drug, being ranked as the 7th most prescribed drug in the USA in 2017 with over 58 million prescriptions. This application brief describes the use of a Quasar C8 column for the analysis of omeprazole in accordance with the United States Pharmacopoeia (USP) monograph.

  • Application Note

    Cannabinoid Monitoring in a Variety of Edibles

    This application describes the sample preparation and analytical method for the chromatographic separation and quantitative monitoring of twelve primary cannabinoids in the extracts of several food matrices by HPLC, using photodiode array (PDA) detection. The method provides exceptional chromatographic repeatability and affords LOQs well below the current concentration levels of interest for cannabinoids in edibles. Thereupon, the method/procedure defined herein can be expected to fulfill the essential task of ensuring product uniformity and cannabinoid screening in edible foods.

  • Application Note

    Rapid UHPLC Determination of Nine Common Herbicides in Drinking Water with the PerkinElmer Flexar FX

    Water polluted by herbicides leach and runoff can cause human health problems including cancer tumors, reproduction deformity, disruption of the endocrine system and DNA damage. This application presents a sensitive and robust liquid chromatography method to test nine widely used herbicides (Figure 1), using a 3 µm UHPLC column to achieve very high throughput at a low flow rate to reduce testing time and solvent consumption. The throughput is compared to that of a conventional C18 HPLC column. Method conditions and performance data including precision and linearity, are presented.

  • Application Note

    HPLC Analysis of Metformin Using a Quasar C18 Column with USP

    Metformin belongs to a class of drugs known as biguanides and contains the active ingredient metformin hydrochloride. Metformin oral tablets are used, in combination with diet and exercise, to treat high blood glucose levels in patients with type 2 diabetes mellitus. Tablets are often used as monotherapy, or in combination with other anti-diabetic agents or insulin.

    This application brief describes the use of a Quasar C18 column for the analysis of metformin hydrochloride RS in accordance with Dissolution Test 3 in the United States Pharmacopoeia (USP) monograph for metformin hydrochloride tablets.

  • Application Note

    Cannabinoid Monitoring in Dried Cannabis Flowers

    This application describes an analytical method for the chromatographic separation and quantitative monitoring of seven primary cannabinoids, including THC and THC-A, in cannabis extracts by HPLC with PDA detection. Naturally occurring cannabinoids, the main biologically active component of the cannabis plant, form a complex group of closely related compounds, of which 113 are known and 70 are well described. Of these, the primary focus has been on ?9-tetrahydrocannabinol (THC), as the primary active ingredient due to its pharmacological and toxicological characteristics, upon which strict legal limits have been enforced.