PerkinElmer
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MaxSignal IAC T-2/HT-2 Toxin

The MaxSignal® T-2/HT-2 Immunoaffinity Column (IAC) kit is designed for quantitative analysis of T-2 and HT-2 Toxins using HPLC or LC-MS/MS.

This kit is intended as a screening tool for research use only, and not intended for clinical diagnostic use.

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FOOD-1512-01
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Overview

This MaxSignal T-2/HT-2 Toxin Immunoaffinity Column can selectively extract T-2 and HT-2 Toxins from the sample solution, thereby having a highly targeted purification effect on the sample. The sample solution that has been purified by passing through the column can be directly used for HPLC or LC-MS/MS analysis after being concentrated with nitrogen gas and resuspended.

T-2 toxin (T-2) is a mycotoxin produced by a variety of Fusaria. It mainly pollutes food crops such as wheat, barley, and corn, and their products, and is relatively harmful to human health and cattle industry. HT-2 toxin is the main metabolite of T-2 toxin. These two toxins are strong inhibitors of protein synthesis in the body and mainly affect the functions of blood, liver, kidney, pancreatic muscle and lymphocytes. The general clinical symptoms after poisoning are anorexia, vomiting, diarrhea, growth arrest, as well as reproduction and neurological dysfunction, etc.

The basis of the IAC measurement is the antigen-antibody interaction. All the main toxin antibodies are embedded throughout the column. After a sample is extracted and filtered, it is slowly passed through the IAC. The toxins bind to their corresponding antibody in the column. The IAC is then washed to remove unbound substances. The toxins are then eluted with the eluent, concentrated using nitrogen gas, then injected into an analytical instrument for detection. Affinity columns can be used in combination with HPLC and LC-MSMS to achieve rapid testing, and to increase signal-to-noise ratio and improve the accuracy of the detection method.

This kit is manufactured to the international quality standard ISO 9001.

Specifications

Product Brand Name MaxSignal
Quantity in a Package Amount 25.0 Box
Sample Type Grain, Feed, Condiment
Sample Volume 3 mL
Shipping Condition Blue Ice
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MaxSignal Immunoaffinity Columns

Combination of Immunoaffinity Column with high performance liquid chromatography (HPLC) or LC-MS/MS is a quantitative method for the detection of mycotoxins in samples such as grains, nuts, feed, dairy and more. Samples are purified by the column and then analyzed with HPLC or LC-MSMS.

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