Spotlight™ IR microscope systems are designed to meet the challenges of an expanding laboratory by generating high-quality, reproducible data from a variety of sample types. The Spotlight 400N FT-NIR Imaging System combines high sensitivity and rapid imaging with ease-of-use. The ability to image large sample areas rapidly at high spatial resolution extends FT-NIR microscopy into new applications.
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FT-NIR Imaging for Exceptional Analyses
The Spotlight 400N FT-NIR Imaging System is designed with state-of-the-art technology to allow for intelligent automation and sophisticated analysis capabilities. The system incorporates a number of unique productivity tools and features an ATR imaging system that enables the collection of high resolution infrared images of extremely small samples.
Unique features of the Spotlight 400N System include:
The Spotlight 400N FT-NIR Imaging System can be configured to meet your FT-NIR microscopy demands and produce high-quality spectra and FT-NIR images from extremely small samples. See the Configurations table below for standard options, and contact a sales representative for more information to customize a system to suit your analysis needs.
Microscopy for a Variety of Applications
Easy to use, yet exceptionally powerful and versatile, the Spotlight’s flexibility and sensitivity make it a perfect addition to any lab setting, in any discipline. Some of the applications supported by the Spotlight 400N System include:
The Spectrum 10 software suite drives the Spotlight 400N system and can support advanced FT-NIR analysis in research and industrial laboratories, always providing optimal results. The software supplied with the system gives you full control over the microscope including the focusing on the sample, illumination, stage position, changing between sampling modes, and manipulating the spectra that you collect. The spectrometers can operate in ratio, single-beam, or interferogram mode. SpectrumIMAGE software enables control over the imaging system with the ability to view sample images with ease in real time. With Spectrum MultiSearch, identification of unknown mixtures is made simple through the use of comparison algorithms allowing for the separation and library matching of up to 10 components.
For regulated industries that demand secure IR quality testing, Spectrum 10 ES™ software is available and encompasses user access control, audit trailing, data security, and data integrity to ensure secure analyses to meet the technical demands of 21 CFR Part 11 compliance.
|21 CFR Part 11 Compatible||Yes|
|Operating Range||5 – 40 °C|
|Product Brand Name||Spotlight|
|Wave Length Range||7,800 – 4,000 cm-1|
|Model||Spotlight Detector||Frontier Options||Standard Features||Optional Extras|
|Spotlight 400N – Frontier NIR||MCT or InGaAs||
|Spotlight 400 – Frontier MIR/NIR||InGaAs||
Human hair is typically 50-100 µ in diameter, previous transmission and ATR studies of its cross section1,2 reveal three major identifiable regions.
Technology improvements and increased use has established chemical imaging as a means of providing information on certain critical quality parameters that is not readily addressable using other techniques.
Application Note, Particle Matter, PM 2.5, Internation Agency on Cancer, IARC, World Health Organization, WHO, PM 10, air sampling, Frontier FT-IR, Spotlight 400, Infrared
The following data was obtained from a sectioned of tomato seed and analyzed using the PerkinElmer spotlight FT-IR imaging system. The section was placed onto a Barium Fluoride window and the infrared data was obtained in transmission mode.
There are many stomata (thousands per square centimeter) in the lower epidermis of plant leaves. Two crescent-shaped cells called guard cells, which are rich in chloroplasts, flank the stoma. Starch is produced in daytime by photosynthesis within the guard cells, not in adjacent epidermal cells.
FT-IR Microscopy quickly provides a unique fingerprint of a complicated material made up of multiple constituents. Another advantage of this technique is that it requires little or no sample preparation and that it can generate spectra in a matter of a few minutes.
Of the several types of counterfeiting defined by the WHO, this application note covers the case where the counterfeit is chemically very similar to the genuine product, containing active ingredient and excipients in the correct concentrations. Traditional techniques such as HPLC and NIR spectroscopy could not differentiate the genuine and counterfeit products. They both work at the macro level, so would show only that the concentrations of ingredients were very similar.
Introducing the PerkinElmer infrared imaging and microscopy systems family.
With Spotlight 400 FT-IR and Spotlight 400N FT-NIR imaging systems, you experience unprecedented, uncompromising data quality and clear, complete, highly detailed results from all your samples. Spotlight FT-IR systems are purpose-built for a wide range of demanding imaging applications. So you’re able to switch between sampling modes – standard transmission, reflection, ATR imaging, and more – with ease, and your images can be collected.
It’s not every day that a masterpiece is created. But how else does one describe a laboratory instrument that will dramatically improve the understanding of materials across an unprecedented range of industries. With the Spotlight™ 400, IR imaging just became faster, more efficient and more flexible than ever before. It is quite simply the most productive laboratory FT-IR imaging system in the world.
A recent study has demonstrated that the accuracy of kidney stone diagnosis can be substantially improved by switching from conventional optical microscopy and contrast staining to infrared (IR) microscopy. There are over a dozen different types of kidney stone, and diagnosing the type is important because most effective treatment methods vary for each.
Analyses using infrared microspectroscopy; performed by the Michigan State Police Forensic; Science Division (MSPFSD); Lansing Laboratory; helped to win a triple murder conviction by matching paint from one victim’s clothing to the murderer’s car.
Among the FDA’s top reasons for product recall were problems with sub-potency and tablet dissolution. In addition, recent cases of cGMP violations have highlighted issues in tablet manufacturing. One area where industry is seeking to improve its processes is in the blending area.
There is an increased interest in the microscopic analysis of samples using FT-IR variable-temperature microscopy or imaging. Application areas include polymer studies, pharmaceutical and food analysis and geological measurements. PerkinElmer offers two options for variable temperature analyses – Linkam variable temperature sample cell and the hot stage accessory.
ATR FT-IR imaging offers users easy spectroscopic access to “difficult” samples which are hard to image in transmission or reflection, or which have confusing depth structure.
ATR FT-IR imaging with germanium crystals offers the potential for acquiring infrared spectral images at up to four times higher spatial resolution than is possible using conventional reflectance or transmission imaging.