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Spotlight 400N FT-NIR Imaging System

Spotlight IR microscope systems are designed to meet the challenges of an expanding laboratory by generating high-quality, reproducible data from a variety of sample types. The Spotlight 400N FT-NIR Imaging System combines high sensitivity and rapid imaging with ease-of-use. The ability to image large sample areas rapidly at high spatial resolution extends FT-NIR microscopy into new applications.

Part Number L1860121
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FT-NIR Imaging for Exceptional Analyses

The Spotlight 400N FT-NIR Imaging System is designed with state-of-the-art technology to allow for intelligent automation and sophisticated analysis capabilities. The system incorporates a number of unique productivity tools and features an ATR imaging system that enables the collection of high resolution infrared images of extremely small samples.

Unique features of the Spotlight 400N System include:

  • High quality spectral production and images from sample areas, yielding pixel resolutions of 6.25, 25, or 50 microns
  • Region of Interest (ROI) finding to allow for ease of analysis of multiple particles and layers all at once
  • Configurability to be used with extended range mid-IR, near-IR or dual range FT-IR with the Frontier™ system to give maximum information from samples in the shortest possible time.
  • Configurability to be used with mid-IR, near-IR, or dual range FT-IR with the Frontier™ or Spectrum Two™ systems to give maximum information from samples in the shortest possible time.

The Spotlight 400N FT-NIR Imaging System can be configured to meet your FT-NIR microscopy demands and produce high-quality spectra and FT-NIR images from extremely small samples. See the Configurations table below for standard options, and contact a sales representative for more information to customize a system to suit your analysis needs.

  • Standard InGaAs (indium gallium arsenide) point and array detector for high-performance near-IR applications
  • Micro-ATR objective with a choice of a silicon or germanium crystal
  • ATR imaging objective with a germanium crystal with automatic switching between wide area survey and high definition imaging modes
  • Standard motorized stage that can be controlled by a joystick or through Spectrum software
  • Standard motorized stage that can be controlled by a joystick or through Spectrum software
  • White light LED illumination for true color display with capabilities for auto-illumination and autofocus modes
  • Spectrum 10™ software as standard with an option for enhanced security in Spectrum 10 ES™
  • SpectrumIMAGE™ software as standard enabling full operation over the imaging system
  • Can be configured as a system with Spectrum Two or purchased as an upgrade package to an existing PerkinElmer FT-NIR spectrometer.

Microscopy for a Variety of Applications

Easy to use, yet exceptionally powerful and versatile, the Spotlight’s flexibility and sensitivity make it a perfect addition to any lab setting, in any discipline. Some of the applications supported by the Spotlight 400N System include:

  • Materials Analysis
  • Forensics
  • Pharmaceuticals
  • Biomedical Research
  • Academic Research
  • Biomaterials

Results Matter

The Spectrum 10 software suite drives the Spotlight 400N system and can support advanced FT-NIR analysis in research and industrial laboratories, always providing optimal results. The software supplied with the system gives you full control over the microscope including the focusing on the sample, illumination, stage position, changing between sampling modes, and manipulating the spectra that you collect. The spectrometers can operate in ratio, single-beam, or interferogram mode. SpectrumIMAGE software enables control over the imaging system with the ability to view sample images with ease in real time. With Spectrum MultiSearch, identification of unknown mixtures is made simple through the use of comparison algorithms allowing for the separation and library matching of up to 10 components.

For regulated industries that demand secure IR quality testing, Spectrum 10 ES™ software is available and encompasses user access control, audit trailing, data security, and data integrity to ensure secure analyses to meet the technical demands of 21 CFR Part 11 compliance.


21 CFR Part 11 Compatible Yes
Depth 57.0 cm
Detector Type InGaAs
Height 47.0 cm
Operating Range 5 – 40 °C
Portable No
Product Brand Name Spotlight
Product Group Instrument
Research Areas Food & Agriculture
Warranty 1 year
Wave Length 10
Wave Length Range 7,800 – 4,000 cm-1
Weight 32.0 kg
Width 34.0 cm


Model Spotlight Detector Frontier Options Standard Features Optional Extras
Spotlight 400N – Frontier NIR MCT or InGaAs
  • Standard NIR system with DTGS detector
  • Spectrum 10
  • SpectrumIMAGE
  • MultiSearch
  • Motorized stage control unit
  • PC with Windows 7 and stage controller
  • AutoATR objective
  • ATR Imaging Accessory
  • NIR reflectance accessory
  • Microscopy polarizer kit
  • Heated stage accessory
  • Micro-sampling tool kit
Spotlight 400 – Frontier MIR/NIR InGaAs
  • System with MIR and NIR DTGS detectors
Resources, Events & More
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Application Note

ATR FT-IR Imaging of Human Hair Cross-Section

Human hair is typically 50-100 µ in diameter, previous transmission and ATR studies of its cross section1,2 reveal three major identifiable regions.

PDF 598 KB
ATR Imaging of Pharmaceutical Tablets

ATR chemical imaging has advanced the state of the art in chemical imaging of certain types of pharmaceutical tablets. It provides a very large improvement in achievable spatial resolution compared with NIR chemical imaging, and sharper, generally easier to interpret images.

Augmenting Standard Methods of Measuring Airborne PM2.5 Using IR Imaging

The use of IR imaging expands the measurement possibilities by giving information about the individual types of materials present in the collected particles. The information from the IR imaging experiment is not only qualitative (i.e. identification of the particulates), but can also be calibrated to give quantitative information on the components present. The IR imaging measurement will take only five minutes, whereas Ion Chromatography requires solvent extraction of the particulates and would take around 20-25 minutes per sample

FT-IR Imaging Dara from Sectioned Tomato Seed

The following data was obtained from a sectioned of tomato seed and analyzed using the PerkinElmer spotlight FT-IR imaging system. The section was placed onto a Barium Fluoride window and the infrared data was obtained in transmission mode.

PDF 346 KB
FT-IR Imaging Study on Stoma of Limonium

There are many stomata (thousands per square centimeter) in the lower epidermis of plant leaves. Two crescent-shaped cells called guard cells, which are rich in chloroplasts, flank the stoma. Starch is produced in daytime by photosynthesis within the guard cells, not in adjacent epidermal cells.

PDF 610 KB
IR Microscopy Used in Effort to Solve Mystery: Did Mallory Climb Everest in 1924?

FT-IR Microscopy quickly provides a unique fingerprint of a complicated material made up of multiple constituents. Another advantage of this technique is that it requires little or no sample preparation and that it can generate spectra in a matter of a few minutes.

PDF 175 KB
Measurement of Counterfeit Pharmaceuticals Using the Spotlight Imaging System

Counterfeiting of pharmaceuticals and the proliferation of substandard drugs constitute a serious health risk for the world population, including both industrialized and developing countries. It is estimated that five to eight percent of the world’s total pharmaceutical sales are counterfeit or of dubious quality. This application note discusses the identification of counterfeit drugs based on differences in the distribution of ingredients within the product.

Polymer Applications Compendium

Today’s plastics are some of the most used materials on a global volume basis. Broadly integrated into today’s industrial and commercial lifestyles, they make a major, irreplaceable contribution to virtually every product category.

In this compendium you will find a wide range of applications for polymers, plastics, rubbers and advanced materials. Discover how to put these applications to work for you simply and efficiently.



Infrared Imaging and Microscopy Systems

Introducing the PerkinElmer infrared imaging and microscopy systems family.

Spotlight FT-IR Microscope & Imaging Systems Interactive Brochure

Materials characterization challenges come in all shapes and sizes – and as labs expand and become more centralized, increased sample volumes get to be a challenge as well. Whether you’re determining composition or identifying defects in advanced materials, performing pharma QA/QC, supporting forensics teams, or engaging in academic research, you need FT-IR instrumentation that’s flexible and easy to operate, getting you ready for whatever comes your way.

Our Spotlight IR microscope and imaging systems are engineered to help you meet your challenges, large and small. These systems deliver simple operation that’s easy enough for novices to perform, clear software controls for any size sample type, streamlined reporting tools, and the flexibility and sensitivity that make it the perfect addition to any lab setting, in any discipline.

Read the interactive brochure to learn more about the Spotlight IR microscopy and imaging solutions to move your science forward.

Spotlight™ 400 FT-IR and 400N FT-NIR Imaging Systems

With Spotlight 400 FT-IR and Spotlight 400N FT-NIR imaging systems, you experience unprecedented, uncompromising data quality and clear, complete, highly detailed results from all your samples. Spotlight FT-IR systems are purpose-built for a wide range of demanding imaging applications. So you’re able to switch between sampling modes – standard transmission, reflection, ATR imaging, and more – with ease, and your images can be collected.

Spotlight™ 400 FT-IR and 400N FT-NIR Imaging Systems Brochure

It’s not every day that a masterpiece is created. But how else does one describe a laboratory instrument that will dramatically improve the understanding of materials across an unprecedented range of industries. With the Spotlight™ 400, IR imaging just became faster, more efficient and more flexible than ever before. It is quite simply the most productive laboratory FT-IR imaging system in the world.


Case Study

Infrared Microscopy Provides More Accurate Kidney Stone Diagnosis

A recent study has demonstrated that the accuracy of kidney stone diagnosis can be substantially improved by switching from conventional optical microscopy and contrast staining to infrared (IR) microscopy. There are over a dozen different types of kidney stone, and diagnosing the type is important because most effective treatment methods vary for each.

PDF 436 KB
Spotlight FT-IR Imaging System Helps Forensic Lab Win Triple Murder Conviction

Analyses using infrared microspectroscopy; performed by the Michigan State Police Forensic; Science Division (MSPFSD); Lansing Laboratory; helped to win a triple murder conviction by matching paint from one victim’s clothing to the murderer’s car.

PDF 306 KB


2020-2022 Materials Characterization Consumables and Supplies Interactive Catalog

Today’s advanced and increasingly diverse Advanced Materials laboratories are facing new challenges on a daily basis – starting from raw materials right up to the finished product. Our comprehensive portfolio of analytical solutions is designed to give you the higher accuracy, sensitivity, and ease of use your laboratory demands for examining with confidence, the purity, composition, and performance of your compounds. What’s more, a range of complementary services is available to keep your laboratory up and running, meeting the stringent requirements of a variety of environments and working practices.

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Tablet uniformity measurements using Near-IR Spectroscopy

Among the FDA’s top reasons for product recall were problems with sub-potency and tablet dissolution. In addition, recent cases of cGMP violations have highlighted issues in tablet manufacturing. One area where industry is seeking to improve its processes is in the blending area.

PDF 308 KB

Product Note

Variable Temperature Stage Options for FT-IR Microscopes and Imaging Systems

There is an increased interest in the microscopic analysis of samples using FT-IR variable-temperature microscopy or imaging. Application areas include polymer studies, pharmaceutical and food analysis and geological measurements. PerkinElmer offers two options for variable temperature analyses – Linkam variable temperature sample cell and the hot stage accessory.

PDF 255 KB

Regulatory Compliance Certification

Technical Note

Large Area ATR FT-IR Imaging Using the Spotlight FT-IR Imaging System

ATR FT-IR imaging offers users easy spectroscopic access to “difficult” samples which are hard to image in transmission or reflection, or which have confusing depth structure.

PDF 702 KB
Spatial Resolution in ATR FT-IR Imaging: Measurement and Interpretation

ATR FT-IR imaging with germanium crystals offers the potential for acquiring infrared spectral images at up to four times higher spatial resolution than is possible using conventional reflectance or transmission imaging.

PDF 264 KB