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Flexar LC

Meet the HPLC system you can count on day after day. For routine analyses, it simply streamlines your processes with easy, trouble-free operation while delivering reliable results every time.

Part Number N2910402
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The need for high performance and reliability are a given for labs that run samples on liquid chromatography instruments. In addition, there are increased efficiency and testing demands across all types of labs that run routine analyses. That makes our proven, rugged and reliable Flexar HPLC system the perfect choice when you need fast cycle times, reproducibility and low carryover. With its elegant streamlined interface, our Flexar LC is easy to use and simplifies your day-to-day processes so you can focus on your applications. Flexar LC provides two chromatography data system (CDS) options, the powerfully easy-to use Chromera® CDS software or TotalChrom® for either single workstation or enterprise-wide control and regulatory compliance.

It’s easy to design precisely the Flexar HPLC system you need for fast, sensitive performance that enables reliable, repeatable sample testing you can count on.


Model Name Quaternary Pump


System Components
Description Part Number


Binary Pump

Quaternary Pump





Standard Autosampler

Peltier Autosampler





Standard Column Oven

Peltier Column Oven





UV/Vis Detector

PDA Plus Detector




Resources, Events & More
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Application Note

Analysis of Cannabinoids in Hemp Seed Oils by HPLC Using PDA Detection

In this application, we describe a technique for the monitoring of six cannabinoids, including THC and CBD, in hemp seed oil by HPLC with PDA detection. Figure 1 shows the chemical structures for the six cannabinoids. In recent years, scientific knowledge regarding the composition and health benefits of edible hemp products has significantly increased. Hemp seed oil has been promoted as a good source of nutritious omega-6 and omega-3 polyunsaturated acids, and may be a cleaner, more sustainable alternative to fish oil.

Analysis of Capsaicin and Dihydrocapsaicin in Chili Peppers Using the PerkinElmer Altus HPLC System with PDA Detection

Capsaicinoids are the compounds that produce the pungency, aroma and flavor of chili peppers. The two most abundant capsaicinoids in chili peppers are capsaicin (8-methyl-N-vanillyl-trans-6-nonenamide) and dihydrocapsaicin. Combined, these two make up close to 90% of the most pungent varieties of capsaicinoids, with capsaisin making up about 71%. The capsaicin content of peppers is one of the parameters that determine their commercial quality. The amount of capsaicin can vary, depending on the light intensity and temperature at which the plant is grown, the age of the fruit, and the position of the fruit in the plant. Besides their widespread uses in foods, capsaicinoids are increasingly being used as the active component in pharmaceuticals and have been used as an analgesic against arthritis pain and inflammation. They have also been reported to be active against neurogenic inflammation (as in pepper sprays) and have shown protective effects against high cholesterol levels and obesity. Considering the increased use of capsaicinoids in both foods and pharmaceuticals, there is an increasing demand for their accurate quantitation as part of monitoring the quality of chili peppers. In this regard, this application focuses on the extraction, HPLC separation and quantitation of capsaisin and dihydrocapsaicin in two store-bought chili pepper powders.

PDF 719 KB
Analysis of Drug Substances in Common Cold Medicines with the PerkinElmer Flexar FX-15 System Equipp

The misuse of cold remedies can be prevented by appropriate medical indications and accurate label claims. To that end, the U.S. Food and Drug Administration and the pharmaceutical industry have made it a standard procedure to routinely test drug products to ensure the accuracy of the amount of active ingredients. This application note presents a method for the simultaneous analysis of acetaminophen, dextromethorphan and phenylephrine. Method conditions and precision are presented. A cold medicine tablet is analyzed and the type and amount of active ingredient are confirmed.

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Analysis of Organic Acids in Fruit Juices by HPLC and UV Detection

Organic acids, used as preservatives, acidulants or stabilizers in food, contribute to the sensory properties of foods and beverages by providing both taste and aroma. Monitoring the content of organic acids in foods is an important step in the food manufacturing process, as well as quality and regulatory protocols, to ensure the purity and composition of finished products. In this application note, a simple and robust HPLC method for the analysis of organic acids in store-bought fruit juices is presented.

Analysis of Steviol Glycosides

With an emphasis on decreasing calorie intake, more and more individuals are focusing on lowering their sugar consumption from foods and beverages. In response to this, food/beverage manufacturers are now introducing the highly touted sugar substitute rebaudioside A (Reb A), in place of all or most of the sugar in certain foods and beverages.,

Cannabinoid Monitoring in Dried Cannabis Flowers

This application describes an analytical method for the chromatographic separation and quantitative monitoring of seven primary cannabinoids, including THC and THC-A, in cannabis extracts by HPLC with PDA detection. Naturally occurring cannabinoids, the main biologically active component of the cannabis plant, form a complex group of closely related compounds, of which 113 are known and 70 are well described. Of these, the primary focus has been on Δ-tetrahydrocannabinol (THC), as the primary active ingredient due to its pharmacological and toxicological characteristics, upon which strict legal limits have been enforced.

Cannabinoid Monitoring in a Variety of Edibles

This application describes the sample preparation and analytical method for the chromatographic separation and quantitative monitoring of twelve primary cannabinoids in the extracts of several food matrices by HPLC, using photodiode array (PDA) detection. The method provides exceptional chromatographic repeatability and affords LOQs well below the current concentration levels of interest for cannabinoids in edibles. Thereupon, the method/procedure defined herein can be expected to fulfill the essential task of ensuring product uniformity and cannabinoid screening in edible foods.

PAHs in Surface Water by PDA and Fluorescence Detection

Polycyclic aromatic hydrocarbons (PAHs) are prevalent in a number of environmental matrices, and are created as a result of the combustion of fossil fuels. PAHs are water soluble and toxic to invertebrate aquatic organisms. They have also been shown to bioaccumulate in the environment, and their solubility results in binding with soils, sediments and particulate matter. Many government agencies, such as the US EPA, have established regulatory limits for PAHs in environmental matrices. In this application, utilizing a spiking experiment, the levels at which PAHs in surface water can be monitored by UHPLC, with a sub-2 µm particle sized column combined with photo diode array (PDA) and fluorescence (FL) detection, are investigated.

Qualitative and Quantitative Analysis of Water-Soluble B Vitamins by HPLC PDA in Various Multivitamin Tablets

The food industry routinely fortifies many foods with vitamins to enhance their nutritional value and to help with deficiencies in dietary requirements. However, to meet legal requirements, the manufacturers must label their products in accordance to the specific regulations pertaining to the country in which the products are sold and consumed. In 2009, the United States Pharmacopeial Convention introduced the USP Dietary Supplements Compendium (DSC) – an industry directed resource, including regulatory guidance and reference tools. In the U.S., the Food and Drug Administration (FDA) regulates all dietary supplement products and manufacturing practices under 21 CFR (Code of Federal Regulation), Part 111. In Europe, the main EU legislation for vitamins and minerals in food supplements is Directive 2002/46/EC. Considering the above, this application focused on providing a robust chromatographic method for the separation of eight water-soluble B vitamins in vitamin supplement tablets using HPLC with photodiode array (PDA) detection.

The Analysis of Lactic and Acetic Acids in Sour Beers by HPLC with PDA Detection

This work pursued an effective, efficient and robust HPLC method for the quantitative analysis of lactic and acetic acid in beers by HPLC with PDA detection.

The Analysis of Lactose in Milk and Cheese Products by HPLC

Lactose, also referred to as milk sugar, is the primary carbohydrate present in dairy products. Globally, the analysis of lactose is especially important due to the many individuals suffering from lactose intolerance. The work presented herein provides a method for the routine sample preparation and quantitative determination of lactose in mozzarella and ricotta cheeses and milk. As the monosaccharaides glucose and galactose can be hydrolytically or enzymatically produced from lactose, these two sugars were also chromatographically separated, though not quantitated. The method uses an Altus HPLC system with refractive index (RI) detector, which is especially effective for analyzing analytes with little or no UV absorption, such as many dietary sugars.

PDF 542 KB
The Analysis of a Broad Range of Organic Acids by HPLC with UV Detection

This application brief provides a routine and reproducible method for the HPLC analysis of 11 organic acids commonly found in juices and other beverages, focusing especially on the relatively higher molecular weight acids.

PDF 987 KB
UHPLC Separation and Detection of Bisphenol A (BPA) in Plastics

BPA or bisphenol A is well known for its effect on human health and is a concern in two common polymers - PVC and Polycarbonate. BPA is used in PVC production as a polymerization inhibitor, however residual BPA monomers may remain after polymerization is complete. The concerns over BPA began with baby bottles and spread to include other consumer products like toys and medical devices. Analysis during plastics development and production can mitigate health concerns. The PerkinElmer Flexar™ FX-10 UHPLC system provides a sensitive and robust platform for this analysis. Demonstrated in this application note is the UHPLC analysis of BPA in 3 children’s toy matrices, with a chromatographic run time of less than 10 minutes. Further, two separate extraction techniques are detailed which simulate potential BPA exposure routes children may encounter. For some applications, companies have replaced polycarbonates with new copolymers, such as co-polyester, to eliminate BPA altogether.



Cannabis Testing Solutions Brochure

Whether your lab is well established or just starting up, having a single-source partner who can offer turnkey solutions that meet the current regulations is essential to a successful business. For years, we’ve worked with government and contract cannabis laboratories to develop industry-leading methods, technology, and exceptional return on investment. We help drive analytical standards and commit to ensuring your laboratory has maximum uptime. Learn about our various testing methods and applications for cannabis analyses. Let us work with you to build an efficient workflow, so you can focus on growing your business and brand.

Comprehensive Testing Solutions for Feed Millers

Feed milling today is a complex business, and agribusinesses both large and small need to balance the nutritional and safety needs of livestock with availability of raw ingredients and their seasonality and variability. Add to that the valuable supplementation and medication that millers supply, and it’s clear: you’re creating a complete nutritional delivery system, not just a simple mix of grains.

Grain Testing Solutions Brochure

The grain industry is very complex. It’s global, diverse, and can also present analytical challenges. Today’s grain users demand more when it comes to quality, safety, and uniformity. In addition, they seek diverse products with unique characteristics.

PerkinElmer is equipped to help the grain industry in its quest to feed the world – nutritiously and economically. Our testing and analysis solutions encompass the three primary areas required for complete knowledge of grains and their derivatives – composition, functionality, and safety.

Meat and Seafood Testing Solutions

Food testing labs like yours are constantly challenged with accurately analyzing samples quickly and efficiently - all while striving to reduce costs due to market forces. Your commitment to ensuring meat and seafood are safe for consumption, as demand increases, is an uphill battle.

Our commitment to you: to provide a range of solutions across multiple technologies, products, and services that meets or exceeds the testing needs of food processors. Our solutions offer more efficiency and increased accuracy and sensitivity for better yields in real time with minimal training.

From instrumentation and software to consumables and reagents to service and support, we are dedicated to providing you with end-to-end solutions that ease your everyday challenges of automation, throughput, service, and time to results.

QSight Triple Quadrupole LC/MS/MS

Whether you’re a lab manager or a bench chemist, you know firsthand how things are changing quickly in food and environmental analysis. And your lab needs to change to keep pace.

Refinery and Natural Gas Analysis

Oil refineries and natural gas producers around the world require their lab operations to perform large numbers of analyses before their products are used in industries and by consumers. Detection of even the slightest impurities, accurate process control and hydrocarbon distribution analysis is critical to the success of these operations.That’s how PerkinElmer can help. As a global scientific leader and solutions provider to refining and natural gas labs, PerkinElmer's proven technology and experience meets the ever-changing needs of the oil and gas industry. PerkinElmer is committed to the success of your oil and gas sample analysis by providing the instrumentation, software, consumables, and services you need for fast, easy and precise testing. The result: better control of your operations and improved product quality.



2020-2021 Chromatography Consumables Interactive Catalog

Today’s advanced and increasingly diverse chromatography laboratories are facing new challenges on a daily basis – starting from raw materials right up to the finished product. Our comprehensive portfolio of analytical solutions is designed to give you the higher accuracy, sensitivity, and ease of use your laboratory demands for examining with confidence, the purity, composition, and performance of your compounds. What’s more, a range of complementary services is available to keep your laboratory up and running, meeting the stringent requirements of a variety of environments and working practices.

Trust the consumables engineered for your instruments.



Palm Oil Analysis. Complete lab solutions from upstream to downstream

Quality control-monitoring and testing are important in ensuring the quality of palm oil. The quality control parameters are used to judge the quality of palm oil products and it can be monitored and tested to ensure that the palm oil is not deliberately or accidentally adulterated.



LC 300 & Quasar LC Columns Promotional Flyer

Whatever your separation challenge, your choice of liquid chromatography (LC) column can make all the difference. Our Quasar portfolio of LC columns combined with the improved work? Workflow and usability of the LC 300 system allows you to achieve rugged and reproducible results. Now, with the purchase of an LC 300 system, you can pick out a Quasar LC column free of charge. Learn more below.

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Regulatory Compliance Certification

Product Certificate - FLEXAR/FX/LC300 UV/VIS Detector

Product Certificate for the Flexar UV/VIS LC Detector, Flexar FX UV/VIS LC Detector and UV/VIS Detector LC 300

PDF 555 KB
Product Certificate - Flexar LC Pumps

Product Certificate for the Flexar LC Pumps

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Product Certificate - Flexar Solvent Manager

Product Certificate for the Flexar Solvent Manager

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Product Certificate - LC PDA Detector

Product Certificate for the LC Flexar PDA Plus Detector, Photodiode Array Detector LC 300, and Multi-Wavelength UV/VIS Detector LC 300

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