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MaxSignal® TMB Microwell Substrate Solution

The MaxSignal® TMB Microwell Substrate Solution is a ready-to-use substrate and chromogen intended for use with peroxidase in microwell applications.

Part Number FOOD-1806-1000
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  • Simplifies ELISA kits for end users - One-component, ready-to-use formulation
  • Proven - Validated in hundreds of different types of ELISA
  • Long shelf life - Stable for 3 years when stored at 2-8ºC
  • Complete - No additional reagents or filtering required
  • Safe - Non-carcinogenic with no DMF or DMSO present in the reagents
  • Assured quality - ISO 9001 certified

TMB is a substrate and chromogen that yields a blue color when oxidized, as a result of oxygen radicals produced by the reduction of hydrogen peroxide by HRP. For kinetic or non-stopped ELISA assays, the TMB chromogen has maximal absorbances at 370 nm and 652 nm. The color then changes to yellow with the addition of sulfuric or phosphoric acid, with maximum absorbance at 450 nm.

One-component, Ready-to-use TMB Formulation Simplifies ELISA Kits.

The MaxSignal® TMB (3,3',5,5' - tetramethylbenzidine) Microwell Substrate Solution is a one-component, ready-to-use liquid formulation that requires no preparation before use. This simplifies ELISA kits for the end user while increasing accuracy by reducing potential errors. Unlike most other commercially available substrates, these products contain no DMF or DMSO.

TMB Validated in Hundreds of Different Types of ELISA Kits.

The MaxSignal TMB Microwell Substrate Solution is a proven, robust solution that has been incorporated into hundreds of different types of ELISA kits developed for both the research and applied markets which have been sold throughout the world for many years. This substrate solution provides high signal and high sensitivity for ELISA kits.

This kit is manufactured to the international quality standard ISO 9001.


Quantity in a Package Amount 1.0 L
Research Areas Food & Agriculture