Designed and developed specifically for the aquaculture industry, the MaxSignal® HTS Nitrofurans and Chloramphenicol ELISA Assays and DS2 Automated ELISA Platform solution delivers a simple, simultaneous 5-in-1 sample preparation method for AOZ, AMOZ, SEM and AHD Nitrofurans as well as Chloramphenicol. This saves time, hands-on requirements, reagents and lab bench footprint and helps support faster and more informed decision making around daily, incoming seafood lots. Cross-contamination will also be reduced and the solution provides excellent sample traceability when using the integrated bar-code scanner and can be linked to LIMS for easy result recording and sharing.
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The MaxSignal® HTS Furazolidone (AOZ) ELISA Kit provides a competitive enzyme immunoassay for the quantitative analysis of AOZ. Furazolidone belongs to the class of nitrofuran antibiotics frequently used in animal production due to their highly efficient antibacterial properties. These drugs are also used as growth promoters during the production of shrimp. However, the parent compounds and their metabolites have been shown to have carcinogenic and mutagenic characteristics, which led to the banning of nitrofurans for the treatment of animals used for food production. In 1993, the EU banned the nitrofurans furaltadone, nitrofurantoin, and nitrofurazone for use in animals used as sources of food, and in 1995, the use of furazolidone was also banned. Therefore, the administration of furaltadone to food-producing animals has been prohibited.
MaxSignal® HTS Furazolidone (AOZ) ELISA Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the entire kit at 2–8°C. Do not use this product past the expiration date indicated on the Certificate of Analysis.
The use of nitrofurans, a class of broad-spectrum antibiotics widely used in the aquaculture industry and their metabolites, has been regulated by several countries and organizations across the world for their harmful effects on human health. ELISA assays are widely used for the detection of nitrofuran metabolites and chloramphenicol for regulatory conformance owing to the high sensitivity, selectivity and ease of use of the method. In this study, we demonstrate the accuracy and precision (CCß validation study) of the simultaneous 5-in-1 sample extraction method by manual ELISA and DS2 automation methods. Sensitivity of the assay kits (LOD) was also demonstrated using manual and DS2 method. Finally, sample variability testing was performed to characterize the effects of matrix from various shrimp sources.
Shrimp aquaculture: As one of Asia’s fastest growing markets, with 10% growth CAGR, it’s a trend that shows no sign of slowing down. To meet this demand, some producers are improving yields through use of antimicrobial compounds.
At the same time, regulations stipulate that seafood processors need to test each outgoing lot to the country of export’s standards for antibiotics, including nitrofurans and chloramphenicol. And that’s where our MaxSignal® HTS automated solution comes in.
This FAQ provides information about the MaxSignal HTS 5-in-1 ELISA and DS2 Automated Laboratory ELISA platform.
Designed and developed specifically for the aquaculture industry, the PerkinElmer MaxSignal® HTS Nitrofurans and Chloramphenicol ELISA Kits and DS2 Automated Laboratory ELISA solution delivers a simple, simultaneous 5-in-1 sample preparation method for AOZ, AMOZ, SEM and AHD Nitrofurans as well as Chloramphenicol.
As a leader in the food and feed safety testing industry, PerkinElmer develops, manufactures, and markets a wide range of seafood diagnostic kits for the detection of antibiotic, histamine, and phycotoxin residues. Consider PerkinElmer your one-stop-shop for seafood safety test kits!
This specification sheet provides information about the MaxSignal HTS 5-in-1 ELISA and DS2 Automated Laboratory ELISA platform.