PerkinElmer
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MaxSignal IAC Fumonisin

The MaxSignal® Fumonisin Immunoaffinity Column (IAC) kit is designed for quantitative analysis of Fumonisins (B1, B2, B3) using HPLC or LC-MS/MS.

This kit is intended as a screening tool for research use only, and not intended for clinical diagnostic use.

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FOOD-1510-01
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Overview

This MaxSignal Fumonisin Immunoaffinity Column can selectively extract Fumonisins (B1, B2, B3) from the sample solution, thereby having a highly targeted purification effect on the sample. The sample solution that has been purified by passing through the column can be directly used for HPLC or LC-MS/MS analysis after concentrating with nitrogen gas and resuspended.

Fumonisin is a metabolite produced by Fusarium fungi. It has acute and chronic toxicities, and is species-specific and organ-specific. Studies have found that high concentrations of fumonisin can cause acute species-specific toxicity symptoms in many domestic and laboratory animals, such as equine leukoencephalomalacia, porcine pulmonary edema, and liver and kidney diseases in sheep. The toxin has also been found to be related to esophageal and liver cancer in humans.

The basis of the IAC measurement is the antigen-antibody interaction. All the main toxin antibodies are embedded throughout the column. After a sample is extracted and filtered, it is slowly passed through the IAC. The toxins bind to their corresponding antibody in the column. The IAC is then washed to remove unbound substances. The toxins are then eluted with the eluent, concentrated using nitrogen gas, then injected into an analytical instrument for detection. Affinity columns can be used in combination with HPLC and LC-MSMS to achieve rapid testing, and to increase signal-to-noise ratio and improve the accuracy of the detection method.

This kit is manufactured to the international quality standard ISO 9001.

Specifications

Product Brand Name MaxSignal
Quantity in a Package Amount 25.0 Box
Sample Type Grain, Feed
Sample Volume 3 mL
Shipping Condition Blue Ice
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MaxSignal Immunoaffinity Columns

Combination of Immunoaffinity Column with high performance liquid chromatography (HPLC) or LC-MS/MS is a quantitative method for the detection of mycotoxins in samples such as grains, nuts, feed, dairy and more. Samples are purified by the column and then analyzed with HPLC or LC-MSMS.

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