Meet the HPLC system you can count on day after day. For routine analyses, it simply streamlines your processes with easy, trouble-free operation while delivering reliable results every time.
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The need for high performance and reliability are a given for labs that run samples on liquid chromatography instruments. In addition, there are increased efficiency and testing demands across all types of labs that run routine analyses. That makes our proven, rugged and reliable Flexar HPLC system the perfect choice when you need fast cycle times, reproducibility and low carryover. With its elegant streamlined interface, our Flexar LC is easy to use and simplifies your day-to-day processes so you can focus on your applications. Flexar LC provides two chromatography data system (CDS) options, the powerfully easy-to use Chromera® CDS software or TotalChrom® for either single workstation or enterprise-wide control and regulatory compliance.
It’s easy to design precisely the Flexar HPLC system you need for fast, sensitive performance that enables reliable, repeatable sample testing you can count on.
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Standard Column Oven
Peltier Column Oven
PDA Plus Detector
In this application, we describe a technique for the monitoring of six cannabinoids, including THC and CBD, in hemp seed oil by HPLC with PDA detection. Figure 1 shows the chemical structures for the six cannabinoids. In recent years, scientific knowledge regarding the composition and health benefits of edible hemp products has significantly increased. Hemp seed oil has been promoted as a good source of nutritious omega-6 and omega-3 polyunsaturated acids, and may be a cleaner, more sustainable alternative to fish oil.
Capsaicinoids are the compounds that produce the pungency, aroma and flavor of chili peppers. The two most abundant capsaicinoids in chili peppers are capsaicin (8-methyl-N-vanillyl-trans-6-nonenamide) and dihydrocapsaicin. Combined, these two make up close to 90% of the most pungent varieties of capsaicinoids, with capsaisin making up about 71%. The capsaicin content of peppers is one of the parameters that determine their commercial quality. The amount of capsaicin can vary, depending on the light intensity and temperature at which the plant is grown, the age of the fruit, and the position of the fruit in the plant. Besides their widespread uses in foods, capsaicinoids are increasingly being used as the active component in pharmaceuticals and have been used as an analgesic against arthritis pain and inflammation. They have also been reported to be active against neurogenic inflammation (as in pepper sprays) and have shown protective effects against high cholesterol levels and obesity. Considering the increased use of capsaicinoids in both foods and pharmaceuticals, there is an increasing demand for their accurate quantitation as part of monitoring the quality of chili peppers. In this regard, this application focuses on the extraction, HPLC separation and quantitation of capsaisin and dihydrocapsaicin in two store-bought chili pepper powders.
The misuse of cold remedies can be prevented by appropriate medical indications and accurate label claims. To that end, the U.S. Food and Drug Administration and the pharmaceutical industry have made it a standard procedure to routinely test drug products to ensure the accuracy of the amount of active ingredients. This application note presents a method for the simultaneous analysis of acetaminophen, dextromethorphan and phenylephrine. Method conditions and precision are presented. A cold medicine tablet is analyzed and the type and amount of active ingredient are confirmed.
In food, organic acids, such as malic, ascorbic and citric acids, originate from natural biochemical processes or are added as preservatives, acidulants and/or stabilizers. Organic acids contribute to the sensory properties of foods and beverages by providing both taste and aroma. In particular, citric acid is widely used in soft drinks to provide the sour citrus taste. The monitoring of these organic acids is essential for quality control during the processing of juices and related products, as well as for evaluating juice authenticity and purity. In addition, the use of organic acids in foods and beverages is regulated in many countries, though regulations vary widely. This application note presents a simple and robust HPLC method for the analysis of organic acids typically found in store-bought fruit juices.
With an emphasis on decreasing calorie intake, more and more individuals are focusing on lowering their sugar consumption from foods and beverages. In response to this, food/beverage manufacturers are now introducing the highly touted sugar substitute rebaudioside A (Reb A), in place of all or most of the sugar in certain foods and beverages.,
This application describes an analytical method for the chromatographic separation and quantitative monitoring of seven primary cannabinoids, including THC and THC-A, in cannabis extracts by HPLC with PDA detection. Naturally occurring cannabinoids, the main biologically active component of the cannabis plant, form a complex group of closely related compounds, of which 113 are known and 70 are well described. Of these, the primary focus has been on ?9-tetrahydrocannabinol (THC), as the primary active ingredient due to its pharmacological and toxicological characteristics, upon which strict legal limits have been enforced.
This application describes the sample preparation and analytical method for the chromatographic separation and quantitative monitoring of twelve primary cannabinoids in the extracts of several food matrices by HPLC, using photodiode array (PDA) detection. The method provides exceptional chromatographic repeatability and affords LOQs well below the current concentration levels of interest for cannabinoids in edibles. Thereupon, the method/procedure defined herein can be expected to fulfill the essential task of ensuring product uniformity and cannabinoid screening in edible foods.
Heightened awareness of polycyclic aromatic hydrocarbons (PAHs) has become prevalent due to urban background levels found in surface water, soil, air, cosmetics and food. They are generated by the combustion of fossil fuels and are always found as a mixture of individual compounds that differ in behavior, environmental distribution, and their effect on biological systems. PAHs encompass a wide molecular weight range, differing based on their physical, chemical, and biological characteristics. PAHs in surface water result from a variety of sources including residential, industrial and commercial outlets, streets and parking lots, and atmospheric fallout. In this application, via a spiking experiment, we explore the levels at which PAHs in surface water can be monitored by UHPLC with a sub-2 µm particle sized column combined with photo diode array (PDA) and fluorescence (FL) detection.,
The food industry routinely fortifies many foods with vitamins to enhance their nutritional value and to help with deficiencies in dietary requirements. However, to meet legal requirements, the manufacturers must label their products in accordance to the specific regulations pertaining to the country in which the products are sold and consumed. In 2009, the United States Pharmacopeial Convention introduced the USP Dietary Supplements Compendium (DSC) – an industry directed resource, including regulatory guidance and reference tools. In the U.S., the Food and Drug Administration (FDA) regulates all dietary supplement products and manufacturing practices under 21 CFR (Code of Federal Regulation), Part 111. In Europe, the main EU legislation for vitamins and minerals in food supplements is Directive 2002/46/EC. Considering the above, this application focused on providing a robust chromatographic method for the separation of eight water-soluble B vitamins in vitamin supplement tablets using HPLC with photodiode array (PDA) detection.
This work pursued an effective, efficient and robust HPLC method for the quantitative analysis of lactic and acetic acid in beers by HPLC with PDA detection.
Lactose, also referred to as milk sugar, is the primary carbohydrate present in dairy products. Globally, the analysis of lactose is especially important due to the many individuals suffering from lactose intolerance. The work presented herein provides a method for the routine sample preparation and quantitative determination of lactose in mozzarella and ricotta cheeses and milk. As the monosaccharaides glucose and galactose can be hydrolytically or enzymatically produced from lactose, these two sugars were also chromatographically separated, though not quantitated. The method uses an Altus™ HPLC system with refractive index (RI) detector, which is especially effective for analyzing analytes with little or no UV absorption, such as many dietary sugars.
This application brief provides a routine and reproducible method for the HPLC analysis of 11 organic acids commonly found in juices and other beverages, focusing especially on the relatively higher molecular weight acids.
Learn more about our various testing methods and applications for cannabis analyses by reading through our Cannabis Testing brochure.
The grain industry is very complex. It’s global, diverse, and can also present analytical challenges. Today’s grain users demand more when it comes to quality, safety, and uniformity. In addition, they seek diverse products with unique characteristics.
PerkinElmer is equipped to help the grain industry in its quest to feed the world – nutritiously and economically. Our testing and analysis solutions encompass the three primary areas required for complete knowledge of grains and their derivatives – composition, functionality, and safety.
Food testing labs like yours are constantly challenged with accurately analyzing samples quickly and efficiently – all while striving to reduce costs due to market forces. In this brochure you can find a range of solutions across multiple technologies, products, and services that meets or exceeds the testing needs of meat and seafood processors. Our solutions offer more efficiency and increased accuracy and sensitivity for better yields in real time with minimal training.
Whether you’re a lab manager or a bench chemist, you know firsthand how things are changing quickly in food and environmental analysis. And your lab needs to change to keep pace.
Oil refineries and natural gas producers around the world require their lab operations to perform large numbers of analyses before their products are used in industries and by consumers. Detection of even the slightest impurities, accurate process control and hydrocarbon distribution analysis is critical to the success of these operations.That’s how PerkinElmer can help. As a global scientific leader and solutions provider to refining and natural gas labs, PerkinElmer's proven technology and experience meets the ever-changing needs of the oil and gas industry. PerkinElmer is committed to the success of your oil and gas sample analysis by providing the instrumentation, software, consumables, and services you need for fast, easy and precise testing. The result: better control of your operations and improved product quality.