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FL 6500 Fluorescence Spectrophotometer with Spectrum FL Software

The FL 6500 Fluorescence Spectrometer is the optimal system for analyzing samples susceptible to photo-bleaching. Photo-bleaching may be experienced with certain thin coatings and compounds in bioresearch, cell-biology, molecular biology, immunology, enzymology, tissue and protein samples; FL 6500 is also excellent for phosphorescence analysis. Our new instrument features intuitive software and 26 diverse plug-n-play accessories that outshine your expectations.

View the interactive online brochure to see how the FL 6500 can support your needs.

Part Number N4200010
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With the new FL 6500 Fluorescence Spectrophotometer instrument your laboratory can generate accurate and reliable fluorescence data, regardless of the operator’s experience level.

Features and Benefits include:

  • Maximize your dynamic range with Pulse long life Xenon lamp with illumination source with selectable Power Settings; power settings range from 20 to 120 Kw
  • High resolution (1.0 nm)
  • High scan speed up to 24,000 nm/min
  • Sensitivity, high performance R928 PMT detector combined with PerkinElmer optics ensures optimum performance across the spectrum.
  • Broad excitation and emission wavelength range up to 900 nm
  • For samples at risk for photo-bleaching the FL 6500 provides accurate and high quality results with a user controllable pulsed excitation source.
  • Superior wavelength accuracy and reproducibility for right first time results
  • 4 collection modes enable you to do more on one instrument: Fluorescence, Phosphorescence, Chemi/Bio-Luminescence, and Time Resolved Phosphorescence measurements
  • Easy to use, your laboratory can generate accurate and reliable fluorescence data, regardless of the operator’s experience level through intuitive Spectrum FL software designed around your workflow.
  • Flexible – 26 and plug-n-play auto-recognized accessories enable you to analyze a wide array of sample types and conditions.
  • Effortless, with numerous preset methods aid you to move between variety of method types.
  • Ideally suited thin coatings, nanoparticles, quantum dots, cell-biology, immunology, molecular biology, enzymology, and tissue & protein analysis.
  • Save time and money with the long life Xenon bulb. When the time does come to replace it save again, you can easily replace the pre-aligned bulb yourself without the assistance of service engineer
  • Peace of mind with Radian, PerkinElmer’s proprietary instrument remote monitoring system
  • Compact footprint to free up valuable bench space (occupies only 66 cm (26 inches) of bench space


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Application Note

Quinine in Tonic Water Application Note

Fluorescence spectroscopy is a very sensitive and selective analytical technique, giving it a major advantage over absorption spectroscopy when used in the detection and measurement of trace amounts of organic compounds. The PerkinElmer FL6500 Fluorescence Spectrometer with Singe Cell accessory provides an accurate, simple and repeatable method for the determination of quinine in tonic water samples.

Validation of the Performance of the FL 6500 and FL 8500 for Pharmaceutical and Industrial Analysis

The US Pharmacopoeia method USP<853> (which is part of USP 40) gives some guidelines for measuring performance of fluorescence spectrometers. The Spectrum FL softwareused for the FL6500 and FL8500 has a validation module which can be used to test key...



Fluorescence Spectroscopy Interactive Brochure

The FL 6500 and FL 8500 use interchangeable, plug-and-play accessories, intuitive software that mirrors your laboratory workflow, and support and services that validate your equipment and ensure standard compliance regulations are met.


Case Study

Biopharmaceutical CMO Bridges Data Integrity Gap

EXELEAD, a biopharmaceutical CMO, knew Data Integrity was an area of vital interest to the FDA during a recent on-site visit and wanted to make sure there were no shortcomings on their end as a part of an ongoing commitment to quality processes.

PDF 299 KB


FL 6500 and 8500 Fluorescence Consumables and Accessories Reference Guide

Today’s advanced and increasingly diverse Advanced Materials laboratories are facing new challenges on a daily basis – starting from raw materials right up to the finished product. PerkinElmer’s comprehensive portfolio of analytical solutions is designed to give you the higher accuracy, sensitivity, and ease of use your laboratory demands for examining with confidence, the purity, composition, and performance of your polymers and compounds. What’s more, a range of complementary services is available to keep your laboratory up and running, meeting the stringent requirements of a variety of environments and working practices. Better insights for better products. Choose PerkinElmer.

The FL 6500 and 8500 are industry-leading fluorescence spectrometers. You’ll be confident in the results you produce with the high-energy pulsed Xenon light source that prevents photobleaching in the FL 6500, along with the FL 8500 system that offers a continuous-wave excitation source that is best for testing diluted or small samples.

PDF 880 KB

Regulatory Compliance Certification


White Paper

Data Integrity – Spectrum FL Enhanced Security (ES) Software for Fluorescence Spectroscopy

The FDA defines data integrity as “completeness, consistency, and accuracy of data. Complete, consistent, and accurate data should be attributable, legible, contemporaneously recorded, original or a true copy, and accurate (ALCOA)”. ALCOA itself has evolved to ALCOA Plus, which incorporates two of the fundamental definition terms as stated by the FDA; complete, consistent, enduring and available. Compliance with 21 CFR Part 11 is mandatory for pharmaceutical companies and their suppliers to sell products into the United States, and also applies to other related industries.

PerkinElmer’s Spectrum™ FL Enhanced Security (ES) software platform for fluorescence spectroscopy provides structural requirements and features to match the specific needs for ensuring data integrity. It affords the system owner the ability to comply with regulations and incorporate features into the validation plan to exhibit compliance. The purpose of this document is to demonstrate how Spectrum FL ES meets the technical requirements for 21 CFR Part 11.