Introduction
The official requirements for the United States Pharmacopeia can be found in the general notices, individual monographs, and general tests and assays chapters. Monographs contain tests, procedures, and criteria to ensure the identity, strength, quality, and purity of the active pharmaceutical ingredient (API) and drug product.
But it isn’t always easy to find all the information necessary to set up a method for a given assay. This is why we’re providing easy-to-follow applications to help your lab adopt and offer new methods for the analysis of a wide variety of pharmaceuticals.
This collection of application briefs and notes demonstrates the use of PerkinElmer LC 300 HPLC System and PerkinElmer Epic™ HPLC Columns, fulfilling all the requirements described in the monographs for the top generic medicines approved by FDA.

HPLC Analysis of Betamethasone Dipropionate Using Epic C18 HPLC Column
Betamethasone dipropionate is classified as a ‘super-potent’ steroid in the treatment of psoriasis in comparison with betamethasone valerate (another common analogue of betamethasone) which is rated as upper mid-strength. This application brief describes the use of an Epic C18 column for the analysis of betamethasone dipropionate in accordance with the official USP monograph.

HPLC Analysis of Acetaminophen, Aspirin and Caffeine Using an Epic C18 Column in Accordance with the USP
This application brief describes the use of an ES Industries Epic C18 column for the analysis of Acetaminophen, Aspirin and Caffeine in accordance with the official Acetaminophen, Aspirin and Caffeine Tablets United States Pharmacopeia (USP) method for the assay.

HPLC Analysis of Finasteride Using an Epic C18 HPLC Column
Finasteride is commonly sold under the brand name Proscar and in 2016 was the 75th most prescribed drug in the USA. This application brief describes the use of an Epic C18 column to analyze finasteride in accordance with the official Finasteride Tablets USP monograph.

HPLC Analysis of Amlodipine Besylate in Accordance with the USP
Amlodipine is listed on the World Health Organization’s (WHO) list of essential medicines as an antihypertensive medicine, meaning that it considered among the safest and most effective compounds needed to meet the requirements of a basic healthcare system. This application brief describes the use of our Epic C18 column to analyze amlodipine besylate in accordance with the official USP monograph.

HPLC Analysis of the Semi-Synthetic Antibiotic Azithromycin Using an Epic C18 Column
The mode of action of azithromycin is like other macrolide antibiotics, it inhibits bacterial protein synthesis by binding to and interfering with the assembly of the 50S large ribosomal subunit of the bacteria, thereby inhibiting translation of mRNA. This application brief describes the use of an Epic C18 column for the simple analysis of azithromycin.

HPLC Analysis of Lamotrigine Related Compound B Impurity in Accordance with the USP
The methods for assessing Lamotrigine related compound B are described in the United States Pharmacopeia1 and rely on chromatographic techniques. This application brief describes use of an Epic C18 column coupled with the LC 300 liquid chromatography system and SimplicityChrom chromatography data system (CDS).

HPLC Analysis of Pregabalin Using Epic C18 Column in Accordance With USP
Pregabalin is an anti-epileptic drug that works by slowing down impulses in the brain that cause seizures. It can also be utilized to treat fibromyalgia, or nerve pain in people with diabetes, herpes zoster, or spinal cord injury owing to its ability to affect chemicals in the brain that send pain signals across the nervous system.

HPLC Analysis of Risperidone Impurities Using an Epic C8 Column in Accordance with United States Pharmacopeia
Easily follow along the USP monograph for the analysis of impurities in Risperidone, an antipsychotic medication. Meet method performance requirements using an LC 300 and Epic C8 column.

HPLC Assay of Risperidone Using an Epic C18 Column in Accordance With the United States Pharmacopeia
Risperidone is an antipsychotic medicine that works by changing the effects of chemicals in the brain. This application brief easily walks through the assay, demonstrating the suitability of the LC 300 instrument and Epic C18 column.

HPLC Analysis of Omeprazole Using an Epic C8 Column in Accordance with United States Pharmacopeia
Navigating USP monographs can be difficult. This Application Brief makes following the USP method for the analysis of Omeprazole easy with an LC 300 instrument and an Epic C8 LC column.

HPLC Assay of Methylphenidate Hydrochloride Using a Chromegabond WR C18 Column in Accordance with United States Pharmacopeia
Use a Chromegabond WR C18 LC column to successfully meet the challenging United States Pharmacopeia (USP) performance requirements for the HPLC analysis of Methylphenidate Hydrochloride.

HPLC Assay of Triamcinolone Acetonide Using an Epic C18 Column in Accordance with United States Pharmacopeia
Use an Epic C18 LC column, LC 300 instrument platform, and SimplicityChrom™ chromatographic data system (CDS) software to successfully meet the United States Pharmacopeia (USP) performance requirements for the HPLC analysis of Triamcinolone Acetonide.

HPLC Assay of Triamcinolone Acetonide Purity Using an Epic C18 Column in Accordance with United States Pharmacopeia
The Epic C18 LC column, LC 300 instrument platform, and SimplicityChrom™ chromatographic data system (CDS) software successfully meet the United States Pharmacopeia (USP) performance requirements for the HPLC analysis of Triamcinolone Acetonide purity.

HPLC Analysis of Ibuprofen Purity Using an Epic C18 Column in Accordance with United States Pharmacopeia
This Application Brief demonstrates the ability of PerkinElmer's Epic C18 LC column, LC 300 instrument platform, and SimplicityChrom™ chromatographic data system (CDS) software to successfully meet the United States Pharmacopeia (USP) performance requirements for the HPLC analysis of Ibuprofen purity.

HPLC Analysis of Ibuprofen Related Compound C Impurity Using an Epic C18 Column in Accordance with United States Pharmacopeia
This Application Brief demonstrates the ability of PerkinElmer's Epic C18 LC column, LC 300 instrument platform, and SimplicityChrom™ chromatographic data system (CDS) software to successfully meet the United States Pharmacopeia (USP) performance requirements for the analysis of Ibuprofen Related Compound C Impurity.