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Spotlight 400 FT-IR Imaging System

Spotlight IR microscope systems are designed to meet the challenges of an expanding laboratory by generating high-quality, reproducible data from a variety of sample types. The Spotlight 400 FT-IR Imaging System combines high sensitivity and rapid imaging with ease-of-use. The ability to image large sample areas rapidly at high spatial resolution extends FT-IR microscopy into new applications.

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FT-IR Imaging for Exceptional Analyses

The Spotlight 400 FT-IR Imaging System is designed with state-of-the-art technology to allow for intelligent automation and sophisticated analysis capabilities. The system incorporates a number of unique productivity tools and features an ATR imaging system that enables the collection of high resolution infrared images of extremely small samples to visualize the composition of materials based on FT-IR spectral data.

Unique features of the Spotlight 400 System include:

  • High quality spectral production and images from sample areas, yielding pixel resolutions of 6.25, 25, or 50 microns
  • Region of Interest (ROI) finding to allow for ease of analysis of multiple particles and layers all at once
  • Configurability to be used with extended range mid-IR, near-IR or dual range FT-IR with the Frontier™ system to give maximum information from samples in the shortest possible time.
  • Configurability to be used with mid-IR, near-IR, or dual range FT-IR with the Frontier™ or Spectrum Two™ systems to give maximum information from samples in the shortest possible time.

The Spotlight 400 FT-IR Imaging System can be configured to meet your FT-IR microscopy demands and produce high-quality spectra and FT-IR images from extremely small samples. See the Configurations table below for standard options, and contact a sales representative for more information to customize a system to suit your analysis needs.

  • Choice of a single element or dual mode MCT (mercury cadmium telluride) detector with an array or a temperature-stabilized DTGS (deuterated triglycine sulfate) as a standard configuration
  • Micro-ATR objective with a choice of a silicon or germanium crystal
  • ATR imaging objective with a germanium crystal with automatic switching between wide area survey and high definition imaging modes
  • Standard motorized stage that can be controlled by a joystick or through Spectrum software
  • Standard motorized stage that can be controlled by a joystick or through Spectrum software
  • White light LED illumination for true color display with capabilities for auto-illumination and autofocus modes
  • Spectrum 10™ software as standard with an option for enhanced security in Spectrum 10 ES™
  • SpectrumIMAGE™ software as standard enabling full operation over the imaging system
  • Can be configured as a system with Spectrum Two or purchased as an upgrade package to an existing PerkinElmer FT-IR spectrometer.

A variety of optional accessories and consumables are available for use with the Spotlight systems, such as an infrared polarizer kit, heated stage accessory, crystal compression cells, and a micro-sampling tool kit for ease with sample handling and preparation.

Microscopy for a Variety of Applications

Easy to use, yet exceptionally powerful and versatile, the Spotlight’s flexibility and sensitivity make it a perfect addition to any lab setting, in any discipline. Some of the applications supported by the Spotlight 400 System include:

  • Materials Analysis
  • Forensics
  • Pharmaceuticals
  • Biomedical Research
  • Academic Research
  • Biomaterials

Results Matter

The Spectrum 10 software suite drives the Spotlight 400 system and can support advanced FT-IR analysis in research and industrial laboratories, always providing optimal results. The software supplied with the system gives you full control over the microscope including the focusing on the sample, illumination, stage position, changing between sampling modes, and manipulating the spectra that you collect. The spectrometers can operate in ratio, single-beam, or interferogram mode. SpectrumIMAGE software enables control over the imaging system with the ability to view sample images with ease in real time. With Spectrum MultiSearch, identification of unknown mixtures is made simple through the use of comparison algorithms allowing for the separation and library matching of up to 10 components.

For regulated industries that demand secure IR quality testing, Spectrum 10 ES™ software is available and encompasses user access control, audit trailing, data security, and data integrity to ensure secure analyses to meet the technical demands of 21 CFR Part 11 compliance.


21 CFR Part 11 Compatible Yes
Depth 57.0 cm
Detector Type MCT
Height 47.0 cm
Operating Range 5 – 40 °C
Portable No
Product Brand Name Spotlight
Product Group Instrument
Technology Type Infrared
Warranty 1 year
Wave Length 7800-600 cm-1
Wave Length Range 7,800 – 600 cm-1
Weight 32.0 kg
Width 34.0 cm


Model Spotlight Detector Spectrum Two System Includes PC with Stage Controller? Includes AutoATR Objective? Part Number Optional Extras
Spotlight 200i – Spectrum Two Standard MCT Standard LiTaO3 detector No No L1862103
  • PC with Windows 7 and motorized stage controller
  • AutoATR objective
  • Microscopy polarizer kit
  • Heated stage accessory
  • Micro-sampling tool kit
Spotlight 200i – Spectrum Two Standard DTGS Standard LiTaO3 detector No No L1862104
Spotlight 200i – Spectrum Two System MCT Temperature-stabilized DTGS detector, APV/AVI Yes No L1862105
Spotlight 200i – Spectrum Two with AutoATR MCT Temperature-stabilized DTGS detector, APV/AVI Yes Yes L1862106

Resources, Events & More

All (20)
Resource Type File Name File Format
Brochure Infrared Imaging and Microscopy Systems PDF  7 MB
Brochure Spotlight™ 400 FT-IR and 400N FT-NIR Imaging Systems PDF  7 MB
Brochure Spotlight™ 400 FT-IR and 400N FT-NIR Imaging Systems Brochure PDF  1 MB
Product Note Variable-temperature Stage Options for FT-IR Microscopes and Imaging Systems PDF  255 KB
Guide Material Characterization Instrument Guide PDF  6 MB
Guide Solutions and Services For Better Results PDF  5 MB
Application Note 006832_01 .qxd PDF  1 MB
Application Note ATR FT-IR Imaging of Human Hair Cross-Section PDF  598 KB
Application Note ATR Imaging of Pharmaceutical Tablets PDF  1 MB
Application Note Augmenting Standard Methods of Measuring Airborne PM2.5 Using IR Imaging PDF  1 MB
Application Note FT-IR Imaging Dara from Sectioned Tomato Seed PDF  346 KB
Application Note FT-IR Imaging Study on Stoma of Limonium PDF  610 KB
Application Note IR Microscopy Used in Effort to Solve Mystery: Did Mallory Climb Everest in 1942? PDF  175 KB
Technical Note Large Area ATR FT-IR Imaging Using the Spotlight FT-IR Imaging System PDF  702 KB
Technical Note Spatial Resolution in ATR FT-IR Imaging: Measurement and Interpretation PDF  264 KB
Poster Tablet uniformity measurements using Near-IR Spectroscopy PDF  308 KB
Case Study Infrared Microscopy Provides More Accurate Kidney Stone Diagnosis PDF  436 KB
Case Study Spotlight FT-IR Imaging System Helps Forensic Lab Win Triple Murder Conviction PDF  306 KB
Product Info Polymer Applications Compendium PDF  11 MB
Regulatory Compliance Certification Spotlight 400 PDF  63 KB