LANCE cAMP Detection Kit, 500 assay points

LANCE cAMP Assay. Red, robust and ready for uHTS! Measure cAMP produced by whole cells or membrane receptors stimulated with GPCR agonists and antagonists with more sensitivity and precision than ever before and outstanding productivity.

PerkinElmer's innovative LANCE® cAMP assay combines homogeneous TR-FRET technology with red-shifted Alexa® Fluor dye chemistry for maximum excitation/emission discrimination. The result is a FRET assay without the compound interference associated with blue dyes. You can read longer without accumulating background signal to maximize S/N and Z'.

Features:

• High sensitivity: fmol detection, leading to improved performance and productivity
• High precision: Z' = 0.8 for Gai cell assay, 0.8 for Gas cell assay
• Flexible sample formats: work with whole cells or membranes
• Easy to automate: simple one-step protocol (following cell stimulation)
• Ultra HTS-compatible: simple conversion between 96-, 384- or 1536-well plate formats results in dramatic savings
• Stable signal (>20 hours at room temperature): ideal for screening
• Read on any TRF-compatible instrument, such as PerkinElmer's ViewLux™, EnVision™, VICTOR3™ and Fusion™ Readers

LANCE cAMP Assay Principle. Light pulse at 340 nm excites the Europium-chelate of the Eu-SA/b-cAMP tracer. The energy emitted from the chelate is transferred to the Alexa-labeled antibodies bound to the tracer, generating a TR-FRET signal at 665 nm. Residual energy from the chelate will produce light at 615 nm. cAMP of a sample competes with the tracer for antibody binding sites and causes signal reduction. This superior performance and ultra high throughput screening convenience leads to dramatic cost savings.