An ELISA or Enzyme Linked Immuno-Sorbent Assay allows for the rapid screening and quantitation of analytes against a variety of materials. We offer a wide range of ELISA kits to measure the safety of dairy, meat, poultry, seafood, grain, feed, and honey.
ELISAs involve at least one antibody with specificity for a particular analyte or antigen and consist of the following basic protocol:
- Liquid sample containing the target analyte is added to a microwell that is pre-coated with analyte-specific antibodies. Any target analyte in the sample binds to the antibodies.
- An enzyme-linked antibody or antigen is added to the microwell, also binding to the analyte specific antibodies coated to the microwell.
- A substrate is added which changes its color as it interacts with the enzymes linked to the analytes or antibodies.
- The color change is measured with a spectrophotometer.
- Quantitation of the analyte is determined by comparing the test sample results against a standard curve of known analyte concentrations.
