FL 6500 Fluorescence Spectrophotometer | PerkinElmer
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FL 6500 Fluorescence Spectrophotometer

The FL 6500 Fluorescence Spectrometer is the optimal system for analyzing samples susceptible to photo-bleaching. Photo-bleaching may be experienced with certain thin coatings and compounds in bioresearch, cell-biology, molecular biology, immunology, enzymology, tissue and protein samples; FL 6500 is also excellent for phosphorescence analysis. Our new instrument features intuitive software and 26 diverse plug-n-play accessories that outshine your expectations.

View the interactive online brochure to see how the FL 6500 can support your needs.

Part Number N4200011
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Overview

With the new FL 6500 Fluorescence Spectrophotometer instrument your laboratory can generate accurate and reliable fluorescence data, regardless of the operator’s experience level.

Features and Benefits include:

  • Maximize your dynamic range with Pulse long life Xenon lamp with illumination source with selectable Power Settings; power settings range from 20 to 120 Kw
  • High resolution (1.0 nm)
  • High scan speed up to 24,000 nm/min
  • Sensitivity, high performance R928 PMT detector combined with PerkinElmer optics ensures optimum performance across the spectrum.
  • Broad excitation and emission wavelength range up to 900 nm
  • For samples at risk for photo-bleaching the FL 6500 provides accurate and high quality results with a user controllable pulsed excitation source.
  • Superior wavelength accuracy and reproducibility for right first time results
  • 4 collection modes enable you to do more on one instrument: Fluorescence, Phosphorescence, Chemi/Bio-Luminescence, and Time Resolved Phosphorescence measurements
  • Easy to use, your laboratory can generate accurate and reliable fluorescence data, regardless of the operator’s experience level through intuitive Spectrum FL software designed around your workflow.
  • Flexible – 26 and plug-n-play auto-recognized accessories enable you to analyze a wide array of sample types and conditions.
  • Effortless, with numerous preset methods aid you to move between variety of method types.
  • Ideally suited thin coatings, nanoparticles, quantum dots, cell-biology, immunology, molecular biology, enzymology, and tissue & protein analysis.
  • Save time and money with the long life Xenon bulb. When the time does come to replace it save again, you can easily replace the pre-aligned bulb yourself without the assistance of service engineer
  • Peace of mind with Radian, PerkinElmer’s proprietary instrument remote monitoring system
  • Compact footprint to free up valuable bench space (occupies only 66 cm (26 inches) of bench space

Specifications

Research Areas Food & Agriculture
Resources, Events & More
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Application Note

Fluorescence Quantification of DNA using PicoGreen and the FL 6500 Spectrometer

Determination of nucleic acids is a common procedure in most molecular laboratories. Accurate and rapid method to quantify DNA is essential to obtain reliable outcomes. Fluorescence spectroscopy offers a simple yet highly sensitive technique to determine low amounts of DNA in solution (even in biolo ...

PDF 948 KB
Fluorometric Determination of Aluminium in Pharmaceutical Products Using the FL6500 Fluorescence Spectrometer

Aluminium salts such as potassium aluminium sulphate, aluminium phosphate and aluminium oxyhydroxide have been extensively used as adjuvants in licensed vaccines. As active ingredients, quantification of aluminium salts in vaccine formulations is required during the manufacturing process. Determinat ...

PDF 1 MB
Protein Quantification Using the FL 6500 Fluorescence Spectrometer and NanoOrange Dye

The application note details a method for quantifying proteins using the FL 6500 Fluorescence Spectrometer and NanoOrange dye. This approach is highlighted for its sensitivity and specificity, especially suitable for detecting low-abundance proteins in complex biological samples. The document outlin ...

PDF 942 KB
Quinine in Tonic Water Application Note

Fluorescence spectroscopy is a very sensitive and selective analytical technique, giving it a major advantage over absorption spectroscopy when used in the detection and measurement of trace amounts of organic compounds. The PerkinElmer FL6500 Fluorescence Spectrometer with Singe Cell accessory prov ...

PDF 1 MB
Thermal Denaturation of protein using the Peltier System coupled with the FL 6500 spectrometer

Development of recombinantly expressed therapeutic proteins as biopharmaceuticals hold the great advantage of biocompatibility and biodegradability. The success of such protein-based drug formulation relies on a deep understanding of the physicochemical stability throughout the production process un ...

PDF 1 MB
Validation of the Performance of the FL 6500 and FL 8500 for Pharmaceutical and Industrial Analysis

The US Pharmacopoeia method USP<853> (which is part of USP 40) gives some guidelines for measuring performance of fluorescence spectrometers. The Spectrum FL softwareused for the FL6500 and FL8500 has a validation module which can be used to test key...

PDF 1 MB

Brochure

Fluorescence Spectroscopy Interactive Brochure

The FL 6500 and FL 8500 use interchangeable, plug-and-play accessories, intuitive software that mirrors your laboratory workflow, and support and services that validate your equipment and ensure standard compliance regulations are met.

PDF 3 MB

Case Study

Biopharmaceutical CMO Bridges Data Integrity Gap

EXELEAD, a biopharmaceutical CMO, knew Data Integrity was an area of vital interest to the FDA during a recent on-site visit and wanted to make sure there were no shortcomings on their end as a part of an ongoing commitment to quality processes.

PDF 299 KB

Flyer

FL 6500 and 8500 Fluorescence Consumables and Accessories Reference Guide

Today’s advanced and increasingly diverse Advanced Materials laboratories are facing new challenges on a daily basis – starting from raw materials right up to the finished product. PerkinElmer’s comprehensive portfolio of analytical solutions is designed to give you the higher accuracy, sensitivity, ...

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Infographic

Regulatory Compliance Certification

PDF 96 KB

White Paper

Data Integrity – Spectrum FL Enhanced Security (ES) Software for Fluorescence Spectroscopy

The FDA defines data integrity as “completeness, consistency, and accuracy of data. Complete, consistent, and accurate data should be attributable, legible, contemporaneously recorded, original or a true copy, and accurate (ALCOA)”. ALCOA itself has evolved to ALCOA Plus, which incorporates two of t ...

PDF 2 MB