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IL-17A homodimer (mouse) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® Mouse IL-17A (Homodimer) Detection Kit is designed for detection and quantitation of mouse interleukin 17A homodimer in cell culture media using a homogeneous (no-wash steps, no separation steps) assay.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
Your Price
100 assay points
876.00 USD
500 assay points
2133.00 USD
5,000 assay points
14200.00 USD
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  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).




  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay


Interleukin-17 (IL-17), a pro-inflammatory cytokine produced in large amounts exclusively by T cells, is part of a family of at least six members (IL-17 A to F) of the IL-17 family. IL-17A is a glycosylated homodimeric protein composed of two 133 amino acid A chains bound by two disulfide bonds. It acts on fibroblasts and macrophages by binding to its receptors to induce the secretion of other pro-inflammatory mediators such as TNF-?, IL-1?, IL-6, IL-8. IL-17 plays critical roles in autoimmune conditions such as lupus erythematosus, rheumatoid arthritis, psoriasis, inflammatory bowel disease, multiple sclerosis, and inflammatory skin diseases. As such, inhibitors have been developed as possible treatments for many of these diseases. IL-17 is also a target for anti-inflammatory therapies to improve recovery post-stroke and to reduce the development of skin cancer. The present kit permits the detection of mouse IL-17A homodimer (i.e. analyte) in mouse serum, plasma, and cell culture supernatants.


AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.


Assay Target IL17A
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Format Microplates
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Inflammation
Unit Size 100 assay points
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Application Brief

Eight Limitations of ELISA and How to Overcome Them Using Alternative Technologies

The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...


Application Note

Quantify Low Abundant mIL-17A Homodimer in Mouse Serum and Plasma

Measuring low levels of biomarkers in complex biological samples such as serum and plasma is always a challenge. This is further complicated by the low sample volumes collected from mice, which are widely used in the early stages of drug discovery and development.

This application note demo ...



Data Sheet