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Interleukin 1a (IL1a) is an 18 kDa pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. The IL1 family includes the agonists IL1a and IL1ß and the antagonist IL1RA. These three molecules compete for the binding to type 1 IL1 receptor (IL-1R1). IL1a is produced as a pro-protein by macrophages and monocytes, and is processed to its active form as a response to cell injury, inducing cell apoptosis. IL1a gene polymorphism has been associated with Alzheimer’s disease and rheumatoid arthritis.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Int ...
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.