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Alliance HIV-1 P24 ANTIGEN ELISA Kit (96 Test)

The Alliance HIV-1 p24 Antigen ELISA is for the detection of HIV-1 and HIV-0 Subtypes in human serum, plasma and cell culture supernatants. This kit provides reagents for immune complex disruption (ICD) of antigen/antibody complexes in serum and plasma samples and proprietary antibodies increasing the sensitivity of the assay.

This kit is intended for research purposes only and is not approved for diagnostic use.

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NEK050001KT
96.0
1379.00 USD
 
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NEK050A001KT
192.0
2305.00 USD
 
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NEK050B001KT
480.0
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Overview

HIV p24 is the 230 amino acid phosphorylated protein of the capsid forming the conical core of the virus that encapsulates the HIV genomic complex. The Alliance HIV-1 p24 Antigen ELISA is for the detection of HIV-1 and HIV-0 Subtypes in human serum, plasma and cell culture supernatants. In gene therapy, HIV-derived lentiviral vectors are useful and commonly used to deliver transgenes to mammalian cells. Assays against the p24 antigen have become a standard method for measuring lentiviral transduction efficiency.

Here are some references demonstrating the p24 Alliance ELISA can be used for lentiviral transduction efficiency:

  • Valkama, A., Leinonen, H., Lipponen, E. et al. Optimization of lentiviral vector production for scale-up in fixed-bed bioreactor. Gene Ther 25, 39–46 (2018).
  • Majdoul S, Cosette J, Seye AK, et al. Peptides derived from evolutionarily conserved domains in Beclin-1 and Beclin-2 enhance the entry of lentiviral vectors into human cells. J Biol Chem. 2017;292(45):18672–18681. doi:10.1074/jbc.M117.800813
  • Lesch, H., Laitinen, A., Peixoto, C. et al. Production and purification of lentiviral vectors generated in 293T suspension cells with baculoviral vectors. Gene Ther 18, 531–538 (2011).
  • Fenard D, Ingrao D, Seye A, et al. Vectofusin-1, a new viral entry enhancer, strongly promotes lentiviral transduction of human hematopoietic stem cells. Molecular therapy. Nucleic Acids. 2013
  • Ausubel LJ, Hall C, Sharma A, et al. Production of CGMP-Grade Lentiviral Vectors. Bioprocess Int. 2012;10(2):32–43.

This kit provides reagents for immune complex disruption (ICD) of antigen/antibody complexes in serum and plasma samples and proprietary antibodies increasing the sensitivity of the assay. This kit is intended for research purposes only and is not approved for diagnostic use.

Specifications

Quantity 96.0
Shipping Condition Blue Ice
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Application Note

A Comparative Study of Two Immunoassay Platforms to Determine Lentivirus Titer for CAR-T Development

The main vectors of gene therapy in research are viruses. The most popular tool for gene delivery is a genetically modified lentivirus. Modified lentivirus (HIV-1) vectors retain their ability to infect undivided cells, thereby increasing their ability to transduce a wide variety of cells, includ ...

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