Liquid Chromatography

Liquid chromatography is a vitally important technology utilized across a number of industries, including food safety, cannabis/hemp production, pharmaceutical development, CMC, manufacturing and QA/QC, environmental monitoring and various industrial and consumer product applications. Although these industries may vary greatly, the LC applications they utilize all demand precision, accuracy and flexibility to meet their unique analytical goals.


Whether your application requires the ruggedness of high-performance liquid chromatography (HPLC), the precision and power of ultra-high performance chromatography (UHPLC), or the flexibility of an HPLC-ICP-MS speciation solution, PerkinElmer offers the right technology to meet your analytical goals. Our analytical solutions, including a full suite of chromatography data system (CDS) software packages, consumables and service offerings, come together to deliver a total liquid chromatography solution to increase your throughput, reduce costs and limit unnecessary complexity in analytical processes.

LC 300 UHPLC System

Our LC 300 UHPLC is the system of choice for applications that demand superior performance and sensitivity. With ultra-precise gradient flows up to 18,000 psi/1,240 bar, the LC 300 UHPLC system delivers the power and increased throughput required for even the most demanding applications.

Key features of the LC 300 UHPLC platform include:

  • 18,000 psi (1240 bar) autosampler and pump configuration.
  • Patented ILD injection valve design reduces pressure and shock to improve data quality and increase column lifetimes.
  • Integrated solvent containment and column oven for reduced system complexity
  • Wide range of detectors: PDA, MWD, UV/Vis, FL and RI
  • Automated sample dilution and derivatization
  • Full instrument control and data processing with the SimplicityChrom CDS software

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LC 300 HPLC System

The next generation LC 300 HPLC system delivers the accuracy and flexibility needed for routine liquid chromatography applications. With its robust feature set and intuitive operation, the LC 300 HPLC has been designed to increase your laboratory productivity and throughput, even in the face of challenging budget and staffing situations.

Key features of the LC 300 HPLC platform include:

  • 10,000 psi (690 bar) autosampler and pump configuration.
  • High visibility color display for immediate assessment of system status without having to log into your CDS software.
  • Outstanding injection reproducibility – even at low volumes
  • Three injection modes: full loop, partial fill and µL-pickup for maximum flexibility
  • Long seal life via a dedicated and automated piston seal wash mechanism
  • Wide range of detectors: PDA, MWD, UV/Vis, FL and RI

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NexSAR Speciation Analysis Ready HPLC System

Next-generation Speciation Analysis Ready system engineered with a completely inert and metal-free fluid path, enabling laboratories to meet low chromatographic background requirements on the most challenging speciation applications.

  • Inert HPLC pump engineered to deliver pulse-free and quiet baselines, with a fluid path that is completely metal-free and composed of inert materials.
  • Inert/cooled inert HPLC autosampler with metal-free and inert fluid path available in either cooled or non-cooled configurations
  • Ergonomically designed solvent tray reduces cavitation and stores solvent bottles in an inert holder, preventing leaks or spillages from damaging the rest of the system.
  • Optional three-channel degasser and solvent tray allows degassing of up to three different solvents, minimizing the risk of air bubbles from entering the system, which would otherwise affect pump and column performance.

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LC 300 Resources

Analysis of Common Preservatives in Personal Care Products by HPLC with UV Detection

Parabens are utilized as an antimicrobial preservative in many commercial offerings including cosmetics, personal care products and foods, and offer both chemical and thermal stability to products. Although parabens increase the safety of products, recent studies have suggested that parabens may exhibit weak endocrine disrupting effects on users of the products, an effect that can be further intensified by the ability of parabens to be absorbed by the skin without being broken done by esterase enzymes. In this application note, a fast, robust and sensitive method utilizing a PerkinElmer LC 300 HPLC system with Multi-Wavelength UV/Vis (MWD) detector for the analysis of 2-phenoxyethanol and seven commonly used parabens in eight different commercially available cosmetic and personal care products is presented.

Analysis of Phytoestrogen Isoflavones in Dietary Supplements by HPLC/UV

Isoflavones are water-soluble compounds found in many plant and food sources. Designated as phytoestrogens, isoflavones are structurally similar to to the female hormone estrogen, and have been shown to exhibit a number of beneficial impacts to human health, such as a reduction in the development of certain cancers, as well as an improvement in cholesterol ratios. Studies also suggest, however, that the phytoestrogenic nature of isoflavones can also lead to an exacerbation of existing thyroid disorders, owing to a disruption to the endocrine system. As such, precise quantification of isoflavone content in nutraceutical products is paramount to ensure label-claim accuracy and consumer safety. In this application note, an efficient and reliable method for the determination of six common isoflavone compounds is presented. The method, which was developed in accordance with the USP monograph for soy isoflavones in dietary supplement capsules, utilizes a PerkinElmer LC 300 UHPLC system, with subsequent detection of compounds achieved utilizing an LC 300 PDA detector.

Analysis of Sugars in Maple Syrup by HILIC with Refractive Index Detection

Maple syrup, a common natural sweetener, is consumed regularly by households around the world. Quantification of sugar content in maple syrup is paramount to satisfy product labeling requirements and ensure label-claim accuracy. Although sucrose is the primary sugar component in maple syrup, a complete identification and quantification of the other complex carbohydrates, including glucose, fructose and maltose, is essential. In this application note, a robust and efficient method for the determination of the four aforementioned complex carbohydrates is presented. Utilizing an LC 300 HPLC system, a hydrophilic interaction chromatography (HILIC) technique is utilized with subsequent detection achieved utilizing an LC 300 Refractive Index (RI) detector.

Analysis of 16 Cannabinoids Using the PerkinElmer LC 300 HPLC System with PDA Detection

Accurate identification and quantification of cannabinoid content is a critical step in the process to bringing safe and high quality cannabis flower and fortified products to market. As the legalization of recreational cannabis use continues throughout the world, fast and efficient liquid chromatography methods for the determination of cannabinoid content are need to satisfy both regulatory and consumer demands. In this application note, a fast and simple method for the determination of 16 common cannabinoids is presented. Utilizing a PerkinElmer LC 300 HPLC system with a PDA detector, the method results in the elution of all sixteen compounds in under seven minutes, with LOQs = 0.15 µg/mL for most analytes.

PerkinElmer LC 300 Autosampler Intermediate Loop Decompression (ILD) Injection Valve

The injection of an accurate and reproducible sample volume into a UHPLC system is challenging, but essential to ensuring a high quality analysis. The patented PerkinElmer Intermediate Loop Decompression (ILD) valve, featured in the LC 300 Autosampler, is a 7-port valve with a strategically placed radial groove in the rotor seal which ensures the injection of an accurate and reproducible sample volume, while also increasing the lifetime of columns used in analysis.

LC Applications

Analysis of Drug Substances in Common Cold Medicines with the PerkinElmer Flexar FX-15 System Equipp

The misuse of cold remedies can be prevented by appropriate medical indications and accurate label claims. To that end, the U.S. Food and Drug Administration and the pharmaceutical industry have made it a standard procedure to routinely test drug products to ensure the accuracy of the amount of active ingredients. This application note presents a method for the simultaneous analysis of acetaminophen, dextromethorphan and phenylephrine. Method conditions and precision are presented. A cold medicine tablet is analyzed and the type and amount of active ingredient are confirmed.

Cannabinoid Monitoring in Dried Cannabis Flowers

This application describes an analytical method for the chromatographic separation and quantitative monitoring of seven primary cannabinoids, including THC and THC-A, in cannabis extracts by HPLC with PDA detection. Naturally occurring cannabinoids, the main biologically active component of the cannabis plant, form a complex group of closely related compounds, of which 113 are known and 70 are well described. Of these, the primary focus has been on Δ-tetrahydrocannabinol (THC), as the primary active ingredient due to its pharmacological and toxicological characteristics, upon which strict legal limits have been enforced.

Analysis of Organic Acids in Fruit Juices by HPLC and UV Detection

Organic acids, used as preservatives, acidulants or stabilizers in food, contribute to the sensory properties of foods and beverages by providing both taste and aroma. Monitoring the content of organic acids in foods is an important step in the food manufacturing process, as well as quality and regulatory protocols, to ensure the purity and composition of finished products. In this application note, a simple and robust HPLC method for the analysis of organic acids in store-bought fruit juices is presented.

PAHs in Surface Water by PDA and Fluorescence Detection

Polycyclic aromatic hydrocarbons (PAHs) are prevalent in a number of environmental matrices, and are created as a result of the combustion of fossil fuels. PAHs are water soluble and toxic to invertebrate aquatic organisms. They have also been shown to bioaccumulate in the environment, and their solubility results in binding with soils, sediments and particulate matter. Many government agencies, such as the US EPA, have established regulatory limits for PAHs in environmental matrices. In this application, utilizing a spiking experiment, the levels at which PAHs in surface water can be monitored by UHPLC, with a sub-2 µm particle sized column combined with photo diode array (PDA) and fluorescence (FL) detection, are investigated.

The Analysis of Sunscreen Active Ingredients and Parabens in Lotions and Lip Balms by UHPLC with PDA Detection

Cosmetics utilized to prevent skin damage from the sun’s radiation often include ingredients which can pose a risk to human health at high enough concentrations. Oxybenzone, for example, can contribute to melanoma associated with the production of DNA-attacking free radicals upon UV exposure. Parabens, utilized as an antimicrobial preservative, have been shown to have estrogenic activity, which could lead to increased risk of breast cancer. Although deemed safe by regulatory bodies such as FDA, manufacturers must monitor the concentration of these and other potentially harmful compounds in their products to ensure levels are at or below regulatory levels. This application note presents a UHPLC method with PDA detection for the analysis of parabens and other active ingredients in a variety of cosmetic products.

NexSAR Resources

Characterization of Arsenic Species in Apple Juice using a NexSAR HPLC-ICP-MS Speciation Analysis Ready Solution

Although apple juice is a healthy, and often preferred, alternative to artificially flavored and carbonated drinks for many parents to give to children, concern has been raised over the presence of arsenic within. This work demonstrates a reversed-phase ion-pairing method for the complete separation and accurate quantification of the main and most toxicologically relevant arsenic species in commercially available apple juices, using a PerkinElmer NexSAR HPLC-ICP-MS system.

Quantification of Low Levels of Hexavalent Chromium in Water Using a NexSAR HPLC-ICP-MS

Due to rising concerns over the carcinogenic properties of hexavalent chromium (Cr6+) in drinking water, many national and regional water standards are looking to lower the maximum allowable levels of total chromium and hexavalent chromium in potable waters. And in order to assess trace concentrations of Cr6+ in drinking water, it is necessary to have an instrument capable of measuring parts per trillion (ppt) concentrations of Cr and possessing a wide linear dynamic range – HPLC-ICP-MS is often the instrumentation of choice in such applications. This work demonstrates an ion exchange method for the characterization of Cr6+ in potable drinking water using our NexSAR HPLC-ICP-MS Speciation Solution. Discover the advantages of the inert and metal-free fluid path of the NexSAR Inert HPLC coupled with the NexION® ICP-MS’ Universal Cell Technology™ for these types of analyses – download this application note.

Speciation of Hexavalent Chromium in Children's Toys According to EN 71-3 Category II with the NexSAR HPLC-ICP-MS

Testing for harmful metal(loid)s that may be included in raw materials, processes, paints and additives used in the manufacturing processes of children's toys has been mandated by the regulation EN 71-3. This regulation recommends specific testing methods and maximum allowable concentrations of leachable metals and metalloids such as Al, As, B, Ba, Cd, Co, Cr (III), Cr (VI), Cu, Hg, Mn, Ni, Pb, Sb, Se, Sn, organotin, Sr and Zn in parts of toys, with the exclusion of general packaging materials. This work shows a method for the measurement of hexavalent chromium in different toy material categories in accordance with method EN 71-3 Category II, leveraging the unique combination and capabilities of the NexSAR™ Inert HPLC coupled to the robust NexION® ICP-MS.

NexSAR HPLC-ICP-MS Speciation Solution

For labs looking to expand their analytical portfolio to include HPLC-ICP-MS, PerkinElmer's NexSAR HPLC-ICP-MS brings together the inert NexSAR speciation analysis ready HPLC system and the revolutionary NexION ICP-MS in a seamless platform, integrated using Clarity chromatography software.

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