PerkinElmer

AlphaLISA Immunoassays

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ELISA, western blot, and other immunodetection technologies have commonly been used for quantitation of biomarkers and other analytes in a variety of sample types. These methods necessitate long, tedious protocols and often provide limited detectable range.

AlphaLISA® provides a homogenous (no-wash) alternative to ELISAs, with a simple, streamlined workflow that can be used to detect and quantitate biomolecules in both simple and complex sample types. AlphaLISA is a bead-based luminescent amplification assay, offering greater sensitivity, a wider dynamic range and small sample sizes over traditional ELISA.

 

In the AlphaLISA assay, a biotinylated antibody and an antibody-conjugated AlphaLISA Acceptor bead are used to capture the target analyte. The biotinylated antibody associates with an Alpha streptavidin-coated Donor bead. When the analyte is present in the sample, the Donor and Acceptor beads are brought together. Upon excitation, a photosensitizer inside the Donor bead converts ambient oxygen to an excited singlet state. Singlet oxygen diffuses up to 200 nm to produce a chemiluminescent reaction in the Acceptor bead, leading to light emission. The amount of light is proportional to the amount of analyte present in your sample.

Alpha offers the benefits over ELISAs including:

  • No-wash
  • Faster time to results
  • Requires a fraction of the sample size, ~5µL
  • Wider dynamic range up to 5 log
  • Improved sensitivity
  • Ability to multiplex
  • Ability to study large protein complexes

For research use only. Not for use in diagnostic procedures.

 

Make the switch to AlphaLISA

Switching to a new immunoassay platform may seem like a large undertaking, but it doesn’t have to be. We have a team of experts ready to help you make the switch. We have a wide range of support options from development guides to or Application Support Knowledgebase (ASK) and from technical support, to our global Field Application Scientist who are hands-on subject matters in assay development.
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OnPoint_2color_100x100

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can custom conjugate your antibody or biomolecule to any bead, or biotinylate or DIG-label your targets. Or maybe you are you short on time and need an assay developed quickly? Custom Alpha assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.
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1-25 of 469 Products & Services

  • PerkinElmer

    AlphaLISA SureFire Ultra Phospho-SHP-2 (Tyr580) Control Lysate

    Extra control lysate for the AlphaLISA SureFire Ultra phospho-SHP-2 (Tyr580) assay. This control is already provided in the AlphaLISA SureFire Ultra phospho-SHP-2 assay kit, but can also be ordered separately.
    List Price : 27.00 USD
  • AlphaLISA phospho-SYK detection kit

    Phospho-SHP-2 (Tyr580) AlphaLISA SureFire Ultra Detection Kit

    The AlphaLISA® SureFire® Ultra™ p-SHP-2 (Tyr580) assay is a sandwich immunoassay for quantitative detection of phospho-SHP-2 (phosphorylated on Tyr580) in cellular lysates using Alpha Technology.
    Price Range : 619.00 USD - 41100.00 USD
  • Alpha SureFire® Ultra™ Multiplex phospho/total SYK Assay Kit

    The Alpha SureFire® Ultra™ Multiplex phospho-SYK (Tyr525/526) + Total SYK assay kit is used to measure both the phosphorylation (on Tyr525/526) and total levels of endogenous SYK in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure SYK phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 1114.00 USD - 71400.00 USD
  • Alpha SureFire® Ultra™ Multiplex phospho/total STAT5 Assay Kit

    The Alpha SureFire® Ultra™ Multiplex phospho-STAT5 (Tyr694/695) + Total STAT5 assay kit is used to measure both the phosphorylation (on Tyr694/695) and total levels of endogenous STAT5 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure STAT5 phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 1114.00 USD - 71400.00 USD
  • Alpha SureFire® Ultra™ Multiplex phospho/total STAT3 Assay Kit

    The Alpha SureFire® Ultra™ Multiplex phospho-STAT3 (Tyr705) + Total STAT3 assay kit is used to measure both the phosphorylation (on Tyr705) and total levels of endogenous STAT3 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure STAT3 phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 1114.00 USD - 71400.00 USD
  • Alpha SureFire® Ultra™ Multiplex phospho/total p70 S6K Assay Kit

    The Alpha SureFire® Ultra™ Multiplex phospho-p70 S6K (Thr389) + Total p70 S6K assay kit is used to measure both the phosphorylation (on Thr389) and total levels of endogenous p70 S6K in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure p70 S6K phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 1114.00 USD - 71400.00 USD
  • Alpha SureFire® Ultra™ Multiplex phospho/total p53 Assay Kit

    The Alpha SureFire® Ultra™ Multiplex phospho-p53 (Ser15) + Total p53 assay kit is used to measure both the phosphorylation (on Ser15) and total levels of endogenous p53 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure p53 phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 1114.00 USD - 71400.00 USD
  • Alpha SureFire® Ultra™ Multiplex phospho/total NFκB Assay Kit

    The Alpha SureFire® Ultra™ Multiplex phospho-NFκB p65 (Ser536) + Total NFκB p65 assay kit is used to measure both the phosphorylation (on Ser536) and total levels of endogenous NFκB in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure NFκB phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 1114.00 USD - 71400.00 USD
  • Alpha SureFire® Ultra™ Multiplex phospho/total MEK1 Assay Kit

    The Alpha SureFire® Ultra™ Multiplex phospho-MEK1 (Ser218/222) + Total MEK1 assay kit is used to measure both the phosphorylation (on Ser218/222) and total levels of endogenous MEK1 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure MEK1 phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 1114.00 USD - 71400.00 USD
  • Alpha SureFire® Ultra™ Multiplex phospho/total eIF2α Assay Kit

    The Alpha SureFire® Ultra™ Multiplex phospho-eIF2α (Ser51) + Total eIF2α assay kit is used to measure both the phosphorylation (on Ser51) and total levels of endogenous eIF2α in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure eIF2α phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 1114.00 USD - 71400.00 USD
  • Alpha SureFire® Ultra™ Multiplex phospho/total ErbB2 Assay Kit

    The Alpha SureFire® Ultra™ Multiplex phospho-ErbB2 (Tyr1221/1222) + Total ErbB2 assay kit is used to measure both the phosphorylation (on Tyr1221/1222) and total levels of endogenous ErbB2 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure ErbB2 phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 1114.00 USD - 71400.00 USD
  • Alpha SureFire® Ultra™ Multiplex phospho/total BTK Assay Kit

    The Alpha SureFire® Ultra™ Multiplex phospho-BTK (Tyr223) + Total BTK assay kit is used to measure both the phosphorylation (on Tyr223) and total levels of endogenous BTK in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure BTK phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 1114.00 USD - 71400.00 USD
  • Alpha SureFire® Ultra™ Multiplex acetyl/total p53 Assay Kit

    The Alpha SureFire® Ultra™ Multiplex p53 (K382Ac) + Total p53 assay kit is used to measure both the acetylation (Lys382) and total levels of endogenous p53 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure p53 acetylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 1114.00 USD - 71400.00 USD
  • AlphaLISA phospho-SYK detection kit

    AlphaLISA SureFire® Ultra™ total mTOR Assay Kit

    The AlphaLISA® SureFire® Ultra™ total mTOR assay kit can be used to measure levels of mTOR (both phosphorylated and non-phosphorylated) in cellular lysates using no-wash Alpha technology.

    This kit has been pre-validated for use in combination with the Pelago Bioscience’s CETSA® (Cellular Thermal Shift Assay) technology. Please note that further assay optimization will be required in order to adapt the kit to your particular cell line and experimental conditions. A valid CETSA® licence from Pelago Bioscience or a single kit agreement is required for using the CETSA® technology.

    Price Range : 619.00 USD - 41100.00 USD
  • AlphaLISA phospho-SYK detection kit

    AlphaLISA SureFire® Ultra™ total ErbB2 Assay Kit

    The AlphaLISA® SureFire® Ultra™ total ErbB2 assay kit can be used to measure levels of ErbB2 (HER2) (both phosphorylated and non-phosphorylated) in cellular lysates using no-wash Alpha technology.
    Price Range : 619.00 USD - 39500.00 USD
  • AlphaLisa Analyte 2

    Cytochrome C (human), lyophilized (AlphaLISA)

    Recombinant human cytochrome C (CYCS) standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human CYCS detection kit, but can also be ordered separately.
    List Price : 1022.00 USD
  • AlphaLisa Analyte 2

    NFL (human), lyophilized (AlphaLISA)

    Recombinant human neurofilament light chain protein (NFL) standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human NFL detection kit, but can also be ordered separately.
    List Price : 1022.00 USD
  • AlphaLISA HIV 1 p24 detection kit

    NFL (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human neurofilament light chain protein (NFL) enables the quantitative determination of human FABP3 in buffer and cerebrospinal fluid (CSF) using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 1051.00 USD - 23600.00 USD
  • AlphaLisa Analyte 2

    FABP3 (human), lyophilized (AlphaLISA)

    Recombinant human fatty acid binding protein 3 (FABP3) standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human FABP3 detection kit, but can also be ordered separately.
    List Price : 1022.00 USD
  • AlphaLISA HIV 1 p24 detection kit

    FABP3 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human fatty acid binding protein 3 (FABP3) enables the quantitative determination of human FABP3 in buffer, cell cultrue media, and serum using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 1008.00 USD - 14900.00 USD
  • AlphaLisa Analyte 2

    IRAK4 (human), lyophilized (AlphaLISA)

    Recombinant human interleukin 1 receptor-associated kinase 4 (IRAK4) standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human IRAK4 detection kit, but can also be ordered separately.
    List Price : 1022.00 USD
  • AlphaLISA HIV 1 p24 detection kit

    IRAK4 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human interleukin 1 receptor-associated kinase 4 (IRAK4) enables the quantitative determination of human IRAK4 in buffer, lysates, and tissue homogenates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 1008.00 USD - 14900.00 USD
  • AlphaLISA phospho-SYK detection kit

    Total SHP-2 AlphaLISA SureFire Ultra Detection Kit

    The AlphaLISA® SureFire® Ultra™ total SHP-2 assay is a sandwich immunoassay for quantitative detection of SHP-2 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-SHP-2 assay.

    This kit has been pre-validated for use in combination with the Pelago Bioscience’s CETSA® (Cellular Thermal Shift Assay) technology. Please note that further assay optimization will be required in order to adapt the kit to your particular cell line and experimental conditions. A valid CETSA® licence from Pelago Bioscience or a single kit agreement is required for using the CETSA® technology.

    Price Range : 619.00 USD - 41100.00 USD
  • AlphaLISA phospho-SYK detection kit

    Total p70 S6K AlphaLISA SureFire Ultra Detection Kit

    The AlphaLISA® SureFire® Ultra™ total p70 S6K assay is a sandwich immunoassay for quantitative detection of p70 S6K (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-p70 S6K assay.

    This kit has been pre-validated for use in combination with the Pelago Bioscience’s CETSA® (Cellular Thermal Shift Assay) technology. Please note that further assay optimization will be required in order to adapt the kit to your particular cell line and experimental conditions. A valid CETSA® licence from Pelago Bioscience or a single kit agreement is required for using the CETSA® technology.

    Price Range : 619.00 USD - 41100.00 USD
  • AlphaLISA phospho-SYK detection kit

    Total NFκB AlphaLISA SureFire Ultra Detection Kit

    The AlphaLISA® SureFire® Ultra™ total NFκB assay is a sandwich immunoassay for quantitative detection of NFκB (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-NFκB assay.
    Price Range : 619.00 USD - 41100.00 USD
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