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Separation buffer containing coating reagent 8 (CR8), a positively-charged additive that binds the silanol groups of the glass channels in the LabChip multi-sipper chip (the glass is negatively-charged) to neutralize the charge. Non-specific interaction of substrates and products that are highly positively charged, or substrates with sequences containing repeats of a positive charge, will be reduced with this separation buffer.

CR-3, or Coating Reagent 3, is an essential component in separation buffer that coats the inner channels of a microfluidic chip to prevent the adsorption and build-up of substrate peptides and other assay components. CR-3 is already present in ProfilerPro Separation Buffer (#760367) at a concentration of 0.1% and does NOT need to be added to Separation Buffer unless you are making your own formulation.