1-6 of 6 Resource Library
Fluorescent nucleotide analogs are biologically active with a variety of DNA and/or RNA polymerizes. Labeling methods such as: nick translation, random priming, polymerase chain reaction, 3'-end labeling, or transcription of RNA using SP6, T3, or T7 RNA polymerases may be used.
Fluorescent 2’, 3’-dideoxynucleotide analogs are biologically active with a variety of DNA polymerases. Since dideoxynucleotides are chain terminators, they result in the analog being extended in a base specific manner onto the 3’-end of the DNA chain.