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  • Technical Note

    Membrane Potential Assays Using the ValiScreen®Human Kv1.3 Voltage-Gated K+ Channel Cell Line on the EnVision Multilabel Plate Reader

    Ion channels are one of the major classes of therapeutic targets due to the central role they play in numerous physiological processes. PerkinElmer’s ValiScreen® cell lines provide an effective means to study compound effects on recombinant ion channels or recombinant G protein-coupled receptors in a well-characterized cell line. In this technical brief we illustrate a membrane depolarization assay performed with the EnVision® Multilabel Plate Reader, describe the optimization of important cell culture parameters, and define the EnVision optical and dispenser settings used to perform this assay.

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