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The AlphaLISA® SureFire® Ultra™ total KAP1 assay is a sandwich immunoassay for quantitative detection of KAP1 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.
This kit has been pre-validated for use in combination with Pelago Bioscience’s CETSA® (Cellular Thermal Shift Assay) technology. Please note that further assay optimization will be required in order to adapt the kit to your particular cell line and experimental conditions. A valid CETSA® licence from Pelago Bioscience or a single kit agreement is required for using the CETSA® technology.
Extra control lysate for the AlphaLISA® SureFire® Ultra™ Total KAP1 assay. This control is already provided in the AlphaLISA SureFire Ultra Total KAP1 assay kit, but can also be ordered separately.