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AlphaLISA technology is a powerful and versatile platform that offers highly sensitive, no-wash immunoassays using Alpha Donor and AlphaLISA Acceptor beads. In this technical note, we present the optimization of an epigenetic enzymatic assay using a biotinylated Histone H3-derived peptide as substrate.
AlphaLISA technology is a powerful and versatile platform that offers highly sensitive, no-wash immunoassays using Alpha Donor and AlphaLISA Acceptor beads. In this technical note, we present the optimization of an epigenetic enzymatic assay using a biotinylated Histone H3-derived peptide as substrate.
AlphaLISA technology is a powerful and versatile platform that offers, highly sensitive, no-wash immunoassays using Alpha Donor and, AlphaLISA Acceptor beads. In this technical note, we present the, optimization of a PRMT6 enzymatic assay using a biotinylated histone, H3-derived peptide as substrate. Detection of the methylated product, was performed by the addition of Streptavidin (SA) Alpha Donor, beads and AlphaLISA Acceptor beads conjugated to an antibody (Ab), directed against the methylated H3R2 residue. Upon laser irradiation, of the beads-target complexes at 680 nm, short-lived singlet oxygen, molecules produced by the Donor beads can reach the Acceptor, beads in proximity to generate an amplified chemiluminescent signal, at 615 nm. The intensity of the light emission is proportional to the, methylation activity of the PRMT6 enzyme.
The AlphaLISA technology allows performing no-wash homogeneous proximity immunoassays using Alpha Donor and AlphaLISA Acceptor beads. In this technical note, we present the optimization of an epigenetic enzymatic assay using a biotinylated histone H3-derived peptide as substrate.
AlphaLISA technology is a powerful and versatile platform that offers highly sensitive, no-wash immunoassays using Alpha Streptavidin Donorand AlphaLISA Acceptor beads. In this technical note, we present the optimization of a signal decrease SIRT1 assay using as substrate abiotinylated p53-derived peptide acetylated at lysine 382.
AlphaLISA technology is a powerful and versatile platform that offers highly sensitive, no-wash immunoassays using Alpha Donor and AlphaLISA Acceptor beads. In this technical note, we present the optimization of an epigenetic enzymatic assay using a biotinylated Histone H3-derived peptide as substrate.
AlphaLISA technology is a powerful and versatile platform that offers highly sensitive, no-wash immunoassays using Alpha Donor and AlphaLISA Acceptor beads. In this technical note, we present,the optimization of an OGA signal-decrease assay using as substrate a biotinylated Tau-derived peptide O-GlcNAcylated at Ser400.
The AlphaLISA technology allows performing no-wash homogeneous proximity immunoassays using Alpha Donor and AlphaLISA Acceptor beads. In this technical note, we present the optimization of anepigenetic enzymatic assay using a biotinylated histone H3-derived peptide as substrate.
AlphaLISA technology is a powerful and versatile platform that offers highly sensitive, no-wash immunoassays using Alpha Streptavidin Donor and AlphaLISA Acceptor beads. In this technical note, we present the qoptimization of a signal decrease HDAC1 assay using as substrate abiotinylated Histone H3-derived peptide acetylated at lysine 27.
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