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Applicability of AlphaLISA Technology to a Wide Spectrum of Complex Biological Samples

Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs. Instead of detection with an HRP substrate, the Alpha assay signal is generated by the excitation of an Eu+-coated bead that has been conjugated to the detection antibody.

Alpha technology offers a simple, straight forward workflow. No wash needed!

1. Add sample
2. Add Acceptor bead mix; incubate 1 hr
3. Add Donor beads; incubate 30 mins
4. Read on Alpha-enable microplate reader

Download this application note to see how no-wash AlphaLISA^® technology provides a more-convenient alternative to ELISA for quantitation of biomarkers in complex sample types, including tissue, serum, and plasma.

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