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The AlphaLISA® SureFire® Ultra™ p-KAP1 (S824) assay is a sandwich immunoassay for quantitative detection of phospho-KAP1 (phosphorylated on S824) in cellular lysates using Alpha Technology.
Extra control lysate for the AlphaLISA® SureFire® Ultra™ Human and Mouse Total Histone H3 assay. This control is already provided in the AlphaLISA SureFire Ultra Human amd Mouse Total Histone H3 assay kit, but can also be ordered separately.
The AlphaLISA® SureFire® Ultra™ total PLC G1 assay is a sandwich immunoassay for quantitative detection of Total (phosphorylated and non-phosphorylated) PLC G1in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.
The AlphaLISA® SureFire® Ultra™ p-PLC G2 (Tyr1197) assay is a sandwich immunoassay for quantitative detection of phospho-PLC G2 (phosphorylated on Tyr1197) in cellular lysates using Alpha Technology.
The AlphaLISA® SureFire® Ultra™ Human Total PKR assay is a sandwich immunoassay for quantitative detection of total PKR in cellular lysates using Alpha Technology.
The AlphaLISA® SureFire® Ultra™ p-FGF Receptor 3 (Y724) assay is a sandwich immunoassay for quantitative detection of phospho-FGF Receptor 3 (phosphorylated on Y724) in cellular lysates using Alpha Technology.
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Cellular kinase signal transduction pathways are involved in the regulation of many important cellular processes such as cell survival, differentiation, and apoptosis. Kinase signaling networks are typically characterized by multiple kinases arranged in cascades containing nodes with feedback loops and crosstalk between pathways. Numerous assay technologies have been developed for studying kinase signaling pathways, and for screening compound libraries in search of agents to modify receptors or kinase activities. The quality of these assays can be impacted by data variability due to cell seeding inhomogeneity or from compound toxicity. A common method for controlling for variability is to normalize the assay signal to a cellular protein whose level does not change as a function of the treatment.
Alpha SureFire® Ultra™ Multiplex Phospho and Total assays utilize two types of Alpha Acceptor beads to simultaneously measure two signals from each assay well to easily normalize the assay. Here, we demonstrate the benefit and utility of normalizing assay signal of phosphorylated protein levels to total protein levels in two different cellular models: ERK 1/2 phosphorylation in human melanoma A375 cells and AKT 1/2/3 phosphorylation in mouse myoblast C2C12 cells.