The AlphaLISA® SureFire® Ultra™ p-GSK-3β (Ser9) assay is a sandwich immunoassay for quantitative detection of endogenous GSK-3β phosphorylated at Ser9 in cellular lysates using Alpha Technology.

The Alpha SureFire^® Ultra™ Multiplex phospho-STAT5 (Tyr694/695) + Total STAT5 assay kit is used to measure both the phosphorylation (on Tyr694/695) and total levels of endogenous STAT5 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure STAT5 phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.

The AlphaLISA^® SureFire^® Ultra^™ total Raf-1 assay is a sandwich immunoassay for quantitative detection to total Raf-1 in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-Raf-1 assay.

The AlphaLISA^® SureFire^® Ultra™ total EGFR assay is a sandwich immunoassay for quantitative detection of EGFR (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-EGFR assay.

The AlphaLISA^® SureFire^® Ultra™ phospho-PD-1 (pan Tyr) assay kit can be used to measure levels of PD-1 (phosphorylated on tyrosine residues) in cellular lysates using no-wash Alpha technology.

The Alpha SureFire^® Ultra™ Multiplex phospho-p70 S6K (Thr389) + Total p70 S6K assay kit is used to measure both the phosphorylation (on Thr389) and total levels of endogenous p70 S6K in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure p70 S6K phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.

CaptSure-coated AlphaPlex-545 (Terbium) Acceptor Beads, for multiplexing SureFire assays. These beads are already included in our Alpha SureFire Ultra Multiplex kits, but can be ordered separately as needed.

The AlphaLISA® SureFire® Ultra™ total CDK4 assay is a sandwich immunoassay for quantitative detection of CDK4 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.

The Alpha SureFire^® Ultra™ Multiplex phospho-SYK (Tyr525/526) + Total SYK assay kit is used to measure both the phosphorylation (on Tyr525/526) and total levels of endogenous SYK in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure SYK phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.

The Alpha SureFire^® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-p70 S6K (Thr389) in a multiplexed assay.

The AlphaLISA® SureFire® Ultra™ total 4E-BP1 assay is a sandwich immunoassay for quantitative detection of 4E-BP1 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.

The AlphaLISA® SureFire® Ultra™ p-FGF Receptor 2 (Y653/654) assay is a sandwich immunoassay for quantitative detection of phospho-FGF Receptor 2 (phosphorylated on Y653/654) in cellular lysates using Alpha Technology.

The AlphaLISA^® SureFire^® Ultra^™ p-AMPKα 1/2 (Thr172) assay is a sandwich immunoassay for quantitative detection of phospho-AMPKα (phosphorylated on Thr172) in cellular lysates using Alpha Technology.

The Alpha SureFire^® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SLP-76 (Ser376) in a multiplexed assay.

The Alpha SureFire^® Ultra™ Multiplex p53 (K382Ac) + Total p53 assay kit is used to measure both the acetylation (Lys382) and total levels of endogenous p53 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure p53 acetylation by either recombinant or endogenous receptors, and can be applied to primary cells.

Extra control lysate for the AlphaLISA SureFire Ultra phospho-VEGFR2 (Tyr1214) assay. This control is already provided in the AlphaLISA SureFire Ultra phospho-VEGFR2 assay kit, but can also be ordered separately.

The AlphaLISA® SureFire® Ultra™ p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.

Extra control lysate for the AlphaLISA SureFire Ultra total ZAP-70 assay. This control is already provided in the AlphaLISA SureFire Ultra total ZAP-70 assay kit, but can also be ordered separately.

The AlphaLISA® SureFire® Ultra™ p-IKKα (Ser176/180) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser176/180) in cellular lysates using Alpha Technology.

The AlphaLISA® SureFire® Ultra™ Multiplex phospho-SMAD2 (Ser465/467) + Total SMAD2 assay kit is used to measure both the phosphorylation of SMAD2 and total levels of endogenous SMAD2 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure SMAD2 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.

The AlphaLISA^® SureFire^® Ultra^™ p-Cofilin (Ser3) assay is a sandwich immunoassay for quantitative detection of phospho-cofilin (phosphorylated on Ser3) in cellular lysates using Alpha Technology.

The Alpha SureFire^® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SMAD1 (Ser463/465) in a multiplexed assay.

The AlphaLISA® SureFire® Ultra™ total JNK1/2 assay is a sandwich immunoassay for quantitative detection of JNK1/2 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.

The AlphaLISA® SureFire® Ultra™ p-MEK1 (Ser218/222) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha no-wash technology.

The AlphaLISA^® SureFire^® Ultra™ total mTOR assay kit can be used to measure levels of mTOR (both phosphorylated and non-phosphorylated) in cellular lysates using no-wash Alpha technology.