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The AlphaLISA® SureFire® Ultra™ total STAT4 assay is a sandwich immunoassay for quantitative detection of STAT4 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.
The AlphaLISA® SureFire® Ultra™ total Acetyl CoA carboxylase assay is a sandwich immunoassay for quantitative detection of Acetyl CoA carboxylase (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.
The AlphaLISA® SureFire® Ultra™ Phospho-RSK1 (Ser380) assay is a sandwich immunoassay for quantitative detection of Phospho-RSK1 (Ser380) in cellular lysates using Alpha Technology.
The AlphaLISA® SureFire® Ultra™ Multiplex phospho-STAT1 (Ser727) + Total STAT1 assay kit is used to measure both the phosphorylation of STAT1 and total levels of endogenous STAT1 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure STAT1 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.
The AlphaLISA® SureFire® Ultra™ total IRAK4 assay is a sandwich immunoassay for quantitative detection of IRAK4 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.
The AlphaLISA® SureFire® Ultra™ total CDK2 assay is a sandwich immunoassay for quantitative detection of CDK2 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.
The Alpha SureFire® Ultra™ Multiplex phospho AMPKα1/2 (Thr172) + Total AMPKα1/2 assay kit is used to measure both the phosphorylation (on Thr172) and total levels of endogenous AMPKα1/2 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure AMPKα1/2 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.
The AlphaLISA® SureFire® Ultra™ Phospho-RSK1 (Thr359/Ser363) assay is a sandwich immunoassay for quantitative detection of Phospho-RSK1 (Thr359/Ser363) in cellular lysates using Alpha Technology.
Extra control lysate for the AlphaLISA® SureFire® Ultra™ Phospho-PKR (Thr451) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-PKR (Thr451) assay kit, but can also be ordered separately.
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Cellular kinase signal transduction pathways are involved in the regulation of many important cellular processes such as cell survival, differentiation, and apoptosis. Kinase signaling networks are typically characterized by multiple kinases arranged in cascades containing nodes with feedback loops and crosstalk between pathways. Numerous assay technologies have been developed for studying kinase signaling pathways, and for screening compound libraries in search of agents to modify receptors or kinase activities. The quality of these assays can be impacted by data variability due to cell seeding inhomogeneity or from compound toxicity. A common method for controlling for variability is to normalize the assay signal to a cellular protein whose level does not change as a function of the treatment.
Alpha SureFire® Ultra™ Multiplex Phospho and Total assays utilize two types of Alpha Acceptor beads to simultaneously measure two signals from each assay well to easily normalize the assay. Here, we demonstrate the benefit and utility of normalizing assay signal of phosphorylated protein levels to total protein levels in two different cellular models: ERK 1/2 phosphorylation in human melanoma A375 cells and AKT 1/2/3 phosphorylation in mouse myoblast C2C12 cells.