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Stably-transfect cells with prepackaged lentivirus particles expressing a combination of novel luciferases and a fluorescent protein for monitoring tumor growth, tracking primary or stem cells in vivo, and other applications.
PerkinElmer’s RediFect™ lentiviral particles contain Red-Fluc, the same red-shifted firefly luciferase (Luciola italica) as our Bioware® Brite cell lines, for a stable bioluminescent signal excellent for deep tissue imaging; or use our green-shifted Renilla luciferase (Renilla reniformis) transgene. Both offer efficient transduction of a wide variety of mammalian cell lines including most cancer cell lines, primary, stem and non-dividing cells. Bioluminescent cells can be imaged using our luciferin or coelenterazine substrates.