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Designed for use with PerkinElmer's IVIS® optical imaging systems, Living Image enables you to analyze 2D and 3D optical imaging data from your animal models with ease. With features such as wizard guidance for acquisition parameter setup and co-registration with other imaging modalities, Living Image allows you to seamlessly capture, visualize and analyze your 2D or 3D optical data to facilitate your drug discovery & development and biology research.
Living Image in vivo software is available for the IVIS Lumina and IVIS Spectrum Series.
Label and track mammalian cells including stem cells, T-cells, macrophages and more with VivoTrack™ fluorescent labeling dye.
VivoTrack 680 is an NIR fluorescent lipophilic dye that intercalates in the cell membrane. With high solubility and excellent stability, VivoTrack offers superior brightness and uniform labeling, ideal for cell detection in vitro and longitudinal cell tracking in vivo across many applications including inflammation, immunology, and stem cell research.
Available as either NHS esters or maleimide reactive dyes, making conjugation to either free amine (-NH3) or free thiol (-SH) containing molecules possible, our VivoTag fluorescent dyes have been validated for in vivo and in vitro applications. Please refer to the configuration table below to see which VivoTag fluorescent agent is best for your research studies.
VivoTag is available in multiple sizes and wavelengths to fit your imaging needs.
1-13 of 13 Resource Library
Fluorescent dyes have been used for many years to label cells for microscopy studies, and a variety of dyes in the visible fluorescence spectrum are available to label different cellular compartments and organelles. Efficient delivery of the fluorophore to the cell without excessively modifying surface proteins or perturbing cell function is the major biotechnological challenge.
VivoTrack™ is a NIR fluorescent dye with a long aliphatic tail to facilitate insertion into the lipid bilayer of the cell. With high solubility, reactivity, and excellent stability, VivoTrack dye offers superior brightness ideal for detection and longitudinal cell tracking in vivo across many applications including inflammation, immunology, and stem cell research.
This application note highlights the benefits of using VivoTrack NIR fluorescent dye in your in vivo imaging studies:
Targeted cancer therapy aims to block key signaling pathways that are critical for tumor cell growth and survival. The blockage eventually results in cell death via apoptosis and eventual tumor growth suppression. This strategy has proven to be quite effective, and the FDA has approved several targeted therapeutics in the past decade. Encouraged by the success in clinical development, many academic and pharmaceutical researchers are in active pursuit of improved next generation targeted anti-cancer drugs. As a result, many new chemical and biological entities are emerging from initial screening of in vitro, in vitro and/or in silico selection processes. From the perspective of drug development, it poses a great challenge for the next stage of in vivo validation and demands a robust, accurate, and efficient method for assessment of these candidates in living animal models.
CAR T therapy has achieved tremendous success in treating blood malignancies, however treating solid tumors with this therapy has proven to be challenging due to several factors such as on-tumor, off-tumor toxicity.
Read this case study where researchers from University of Pennsylvania created a mouse model that expresses tumor associated antigens in normal tissue to study off-tumor CART-T cell toxicity. These studies used optical imaging on the IVIS® platform to longitudinally monitor off-tumor antigen expression, tumor progression, and CAR-T cell trafficking in live animals.
With the potential to treat a wide range of disease, from organ damage to congenital defects, stem cell research and tissue engineering form the underlying basis of regenerative medicine. Significant advances in the science of skin regeneration, for example, have now made it possible to develop and grow artificial skin grafts in a lab for treatment of burn victims. Other therapeutic applications include the use of stem cells to treat and repair central nervous system diseases such as ischemia and cerebral palsy, cardiovascular diseases, as well as autoimmune diseases including type I diabetes.
Epifluorescence (2D) imaging of superficially implanted mouse tumor xenograft models offers a fast and simple method for assessing tumor progression or response to therapy. This approach for tumor assessment requires the use of near infrared (NIR) imaging agents specific for different aspects of tumor biology, and this Application Note highlights the ease and utility of multiplex NIR fluorescence imaging to characterize the complex biology within tumors growing in a living mouse.
In vivo optical imaging can provide information at the cellular biomarker level regarding disease states and therapeutic response. Bioluminescence imaging presents few challenges with respect to imaging and data acquisition. However, fluorescence imaging requires strategies to compensate for background fluorescence. Without proper background subtraction, results may underestimate biological changes or the magnitude of therapeutic efficacy of a drug. There are several contributors to background fluorescence which can vary depending on disease model and probe used all of which need to be considered when developing a mouse model for fluorescence imaging. Learn about key considerations for defining and applying background correction to improve fluorescence quantification and interpretation using the IVIS® platform in this technical note.
Targeted cancer therapy aims to block key signaling pathways that are critical for tumor cell growth and survival. The blockage eventually results in cell death via apoptosis and tumor growth suppression. Encouraged by the success in clinical development, many academic and pharmaceutical researcher are in active pursuit of the improvement of next generation targeted anti-cancer drugs. As a result, many new chemical and biological entities are emerging from initial screening of in vitro, in vitro and/or in silico selection processes. From the perspective of drug development, it poses a great challenge on the next stage of in vivo validation and demands a robust, accurate, and efficient method for assessment of these candidates in living animal models.
Bone erosion is a pathological condition characterized by breaks in the cortical bone surface and loss of the adjacent trabecular bone. Several pathological processes can lead to bone erosion, including malignant tumors, abnormal metabolic processes such as hyperparathyroidism, and chronic inflammatory diseases such as rheumatoid arthritis. In clinical settings, bone erosion is routinely detected using X-ray based imaging technologies such as computed tomography (CT). Although preclinical CT offers high resolution 3D imaging for bone, accessibility to this modality may be challenging. Learn how optical and high-resolution X-ray imaging capability on the IVIS Lumina X5 system and Living Image® software was used for obtaining high quality images for quantitative analysis in a mouse bone erosion model, with the ease and speed of 2D imaging.
Cancer chemotherapy can produce severe side effects such as suppression of immune function and damage to heart muscle, gastrointestinal tract, and liver. If serious enough, tissue injury can be a major reason for late stage termination of drug discovery research projects, so it is becoming more important to integrate safety/toxicology assessments earlier in the drug development process. There are a variety of traditional serum markers, tailored mechanistically to specific tissues, however there are no current non-invasive assessment tools that are capable of looking broadly at in situ biological changes in target and non-target tissue induced by chemical insult.
Obesity is a global epidemic that is the fifth leading cause of death worldwide and in the US alone, nearly 85% of adults are expected to be overweight or obese by 2030. In addition to the increased risk of overall mortality, obesity is associated with an increased risk for other metabolic disorders such as diabetes and heart disease which pose a significant burden to health care systems.
Read this case study to learn how researchers at the Joslin Diabetes Center in Boston, Massachusetts used the IVIS® optical imaging system to visualize and quantify CRISPR/Cas9 engineered adipose tissue in a mouse model to study the prevention of obesity and obesity-related metabolic disorders.
Drug induced liver injury (DILI) is a major reason for late stage termination of drug discovery research projects, highlighting the importance of early integration of liver safety assessment in the drug development process. A technical approach for in vivo toxicology determination was developed using Acetaminophen (APAP), a commonly used over-the-counter analgesic and antipyretic drug, to induce acute hepatocellular liver injury.
The collection of app notes focuses on how we can quickly analyze cellular images through machine-assisted learning and characterize profile, and test for the efficacy of the delivery vehicle and transgene using our latest technologies.
Optical-based in vivo imaging of vascular changes and vascular leak is an emerging modality for studying altered physiology in a variety of different cancers and inflammatory states. A number of fluorescent imaging probes that circulate with the blood, but have no target selectivity, have been used to detect tumor leakiness as an indication of abnormal tumor vasculature. Inflammation is also characterized by distinct vascular changes, including vasodilation and increased vascular permeability, which are induced by the actions of various inflammatory mediators. This process is essential for facilitating access for appropriate cells, cytokines, and other factors to tissue sites in need of healing or protection from infection. This application note investigates the use of three fluorescent imaging probes, to detect and monitor vascular leak and inflammation in preclinical mouse breast cancer models.