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1-25 of 54 Business Insights

  • Poster

    Quasar Liquid Chromatography Columns Postcard

    SPECIAL OFFER: 35% off discount on all Quasar LC Columns Until August 28, 2020 To take advantage of this offer, visit our website and use promotion code: QUASAR35 at checkout.

  • Application Note

    Analysis of 24 Polar and Non-Polar Volatile Organic Compound (VOCs) Emissions from Stationary Source

    Volatile organic compounds (VOCs) have been recognized as a primary source of air pollution, and are known photochemical reaction precursors which facilitate the production of tropospheric, or ground-level, ozone. Ground-level ozone can have significant impacts on human health, and contributes to climate change throughout the world. VOC emissions from stationary sources, such as ducts, stacks and exhaust funnels in factories, refineries and mines, have been identified as significant contributors to air pollution and ground-level ozone formation. In this paper, a qualitative and quantitative method for the analysis of 24 polar and nonpolar VOCs was established using a PerkinElmer TurboMatrix ATD and a PerkinElmer Clarus® GC/MS with electron ionization (EI) source. The method is simple, sensitive and efficient to comply with regulatory requirements.

  • Application Note

    Quantification of Low Levels of Hexavalent Chromium in Water Using a NexSAR HPLC-ICP-MS

    Due to rising concerns over the carcinogenic properties of hexavalent chromium (Cr6+) in drinking water, many national and regional water standards are looking to lower the maximum allowable levels of total chromium and hexavalent chromium in potable waters. And in order to assess trace concentrations of Cr6+ in drinking water, it is necessary to have an instrument capable of measuring parts per trillion (ppt) concentrations of Cr and possessing a wide linear dynamic range – HPLC-ICP-MS is often the instrumentation of choice in such applications.

    This work demonstrates an ion exchange method for the characterization of Cr6+ in potable drinking water using our NexSAR HPLC-ICP-MS Speciation Solution. Discover the advantages of the inert and metal-free fluid path of the NexSAR Inert HPLC coupled with the NexION® ICP-MS’ Universal Cell Technology for these types of analyses – download this application note.

  • Flyer

    Quasar Liquid Chromatography Columns Flyer

    Our wide range of column sizes has its benefits, including:• Longevity to withstand highthroughput environments• High-efficiency separations to facilitate trace-level analysis• Good resolution and peak shapes for more effective compound separation• Analysis of increasing polar and complex analytes.

  • Application Brief

    Arsenic Speciation Analysis in Rice Following Method GB 5009.11-2014 to Ensure Safety

    Rice represents a dietary staple for over half of the world's population. However, in recent years, concerns have been raised over the presence of high levels of arsenic (As), where this carcinogenic metalloid can be accumulated at levels far exceeding ambient concentrations. Since China is the world's largest producer of rice, the Chinese government released a national standard method in 2014 for food safety – GB 5009.11– to aid the evaluation of As species in rice and rice products. In this document, HPLC-ICP-MS is identified as the preferred analytical technique.

    In this study, five arsenic species were characterized in a commercial rice sample and certified reference material using a gradient anion-exchange method in accordance with GB 5009.11. The analysis was performed using a PerkinElmer NexSAR HPLC-ICP-MS speciation solution, consisting of a NexSAR inert HPLC coupled to a NexION® ICP-MS.

  • Application Brief

    HPLC Analysis of Plavix Using Quasar C8 Column

    The medication known as Plavix is an antiplatelet drug which prevents platelets in your blood from sticking together to form an unwanted blood clot, that could block an artery. It is used to lower the risk of having a stroke, or serious heart problem after you've had a heart attack, or aid with circulation problems as it helps to keep blood flowing smoothly in the body. The patent expired in 2012 and the drug is now also sold under many generic brands. Plavix tablets contain the active ingredient clopidogrel hydrogen sulphate, which is readily analysed by HPLC. This application brief describes use of a Quasar C8 column in the analysis of Plavix.

  • Application Brief

    HPLC Analysis of Fluconazole Using Quasar C18 Columns in Accordance with USP Application Brief

    Fluconazole is an azole anti-fungal medication used primarily in the treatment of a wide range of fungal infections. Examples include thrush, urinary tract infections and the prevention of fungal infections in immunocompromised patients undergoing chemotherapy. The mechanism of action can be described as fungistatic, the growth and replication of fungi is inhibited by fluconazole rather than directly attacked. This process is achieved by inhibiting enzymes which are important in the production of fungal membranes, allowing the immune system ample opportunity to target and degrade the pathogen. Fluconazole has generic status and is thus globally produced and prescribed. It is on the World Health Organization’s (WHO) list of essential medicines, which serves as a model of the safest and most effective medications needed by a health system. This application brief describes the use of a Quasar C18, on both porous silica and SPP silica phases, for the analysis of fluconazole in accordance with the official USP monograph.

  • White Paper

    Advantages and Applications of Superficially Porous Particle Columns in Liquid Chromatography

    The current trend in liquid chromatography is towards the achievement of higher kinetic efficiency and shorter analysis time. Different types of column packings are now available for attaining very fast and high resolution separations without changing instruments, worrying about high backpressure or compromising column longevity. In recent developments of particle technology, the use of superficially porous particles has received considerable attention. This white paper gives an overview about the theory behind the success of superficially porous particles technology and presents a summary of its latest applications.

  • Application Note

    Characterization of Arsenic Species in Apple Juice using a NexSAR HPLC-ICP-MS Speciation Analysis Ready Solution

    Although apple juice is a healthy, and often preferred, alternative to artificially flavored and carbonated drinks for many parents to give to children, concern has been raised over the presence of arsenic within. This work demonstrates a reversed-phase ion-pairing method for the complete separation and accurate quantification of the main and most toxicologically relevant arsenic species in commercially available apple juices, using a PerkinElmer NexSAR HPLC-ICP-MS system.

  • Application Brief

    HPLC Analysis of Tramadol Using SPP Column Technology

    Opiates, originally derived from the opium poppy, have been used for thousands of years for both recreational and medicinal purposes.

  • Application Brief

    Rapid and Robust HPLC Analysis of Indoxyl Sulfate Using Quasar C18 Column

    Indoxyl sulfate is one of the most extensively studied solutes that accumulates in the plasma when the kidneys fail. Originally called "indican", it was first isolated by Obermayer and Popper in 1911. High concentrations of indoxyl sulfate in blood plasma are known to be associated with the development and progression of a number of pathological conditions including chronic kidney disease and vascular disease. The scientific literature documents the use of older generation C18 columns, using type A silicas. This application brief will illustrate the application of a new generation C18 column, based on type B silica, for the analysis of indoxyl sulfate, Figure 1, as part of a research study to measure the total levels in a simulated blood serum environment.

  • Application Note

    A Quantitative Determination of Methadone and its Metabolite (EDDP) in Dry Blood Spots Using LC-MSMS

    The present research study provides a much simplified approach whereby a highly sensitive LC-MS/MS triple quadrupole mass spectrometer is used to measure directly the methadone and EDDP concentrations through the dried blood spot (DBS) samples.

  • Application Brief

    HPLC Analysis of the Semi-Synthetic Antibiotic Azithromycin Using a Quasar C18 Column

    Azithromycin, discovered in 1980, is a semi-synthetic macrolide antibiotic derived from erythromycin. The drug is primarily used to treat various bacterial infections including respiratory infections, acute bacterial sinusitis, skin infections, Lyme disease, and some sexually transmitted infections. The mode of action of azithromycin is by inhibiting bacterial protein synthesis through binding to the 50S subunit of the bacterial ribosome, thereby inhibiting translation of mRNA. The USP monograph for the analysis of azithromycin states the use of a high pH mobile phase (pH 11), which requires a specific and expensive column. The method also uses amperometric electrochemical detection, which is often unavailable in many laboratories. HPLC with a UV or PDA detector provides a good alternative as it is available in most laboratories. This application brief describes the use of a Quasar C18 column in the analysis of azithromycin.

  • Application Note

    Rapid Determination of Trace Amounts of Aniline and Its Derivatives by Direct Injection UHPLC/MS/MS

    Aniline and aniline derivatives are widely used as raw materials and intermediates in the polymer, rubber, dye, pesticide and pharmaceutical industries. Unintended releases of aniliane or its derivatives into the environment pose a serious threat, with release possible during any stage of production, storage, transport, use, or disposal. Therefore, it is important and necessary to develop fast, simple, sensitive, selective and efficient methods for the determination of aniline and its derivatives in drinking and environmental waters. The aim of this study is to develop an LC/MS/MS method for rapid analysis of aniline and its derivatives in water samples by direct injection, without using time consuming sample preparation steps.

  • Application Note

    High Sensitivity Methotrexate Quantitation in Human Serum Utilizing

    This application note outlines a rapid LC-MS/MS research method utilizing the QSight® 220 triple quadrupole mass spectrometer. The developed method provides exceptional results for the quantitation of methotrexate in serum especially in terms of LLOQs and linearity.

  • Application Note

    HPLC Analysis of Metformin Using a Quasar C18 Column with USP

    Metformin belongs to a class of drugs known as biguanides and contains the active ingredient metformin hydrochloride. Metformin oral tablets are used, in combination with diet and exercise, to treat high blood glucose levels in patients with type 2 diabetes mellitus. Tablets are often used as monotherapy, or in combination with other anti-diabetic agents or insulin.

    This application brief describes the use of a Quasar C18 column for the analysis of metformin hydrochloride RS in accordance with Dissolution Test 3 in the United States Pharmacopoeia (USP) monograph for metformin hydrochloride tablets.

  • Application Note

    Speciation of Hexavalent Chromium in Children's Toys According to EN 71-3 Category II with the NexSAR HPLC-ICP-MS

    Testing for harmful metal(loid)s that may be included in raw materials, processes, paints and additives used in the manufacturing processes of children's toys has been mandated by the regulation EN 71-3. This regulation recommends specific testing methods and maximum allowable concentrations of leachable metals and metalloids such as Al, As, B, Ba, Cd, Co, Cr (III), Cr (VI), Cu, Hg, Mn, Ni, Pb, Sb, Se, Sn, organotin, Sr and Zn in parts of toys, with the exclusion of general packaging materials.

    This work shows a method for the measurement of hexavalent chromium in different toy material categories in accordance with method EN 71-3 Category II, leveraging the unique combination and capabilities of the NexSAR Inert HPLC coupled to the robust NexION® ICP-MS.

  • Technical Note

    A Comparison of Quasar C18 and Inertsil ODS-3 HPLC Columns

    The most commonly used LC reversed-phase alkyl bonded stationary phase is octadecyl carbon chain (C18)-bondedsilica, which is denoted as USP classification L1. C18 columns have a broad applicability from pharmaceuticals to food and environmental analyses. However, not all C18 columns are alike. Simply swapping a C18 column from one manufacturer to another can result in differences in retention time, resolution and even selectivity. Differences can arise due to variations in hydrophobicity, silanol activity, packing quality, particle size distribution, and silica purity, to name a few.1

    This technical note provides details of a comparative study between PerkinElmer Quasar™ C18 and GL Sciences Inertsil ODS-3 silica-based columns (150 x 4.6 mm, 5 µm), covering the following areas:

    • Relative Hydrophobicity
    • Efficiencies for a Neutral Compound
    • Evaluation of Silanol Activity
    • Application example – analysis of clotrimazole

  • Application Brief

    Warfarin Analysis Using a Quasar AQ UHPLC Column

    Today, Warfarin is the most widely used anticoagulant in the world, used to thin the blood and prevent clots.1 It was discovered by chance when in the 1920’s cattle in the US were found to be bleeding to death having eaten mouldy hay from sweet clover crops.2 However, the exact identity of the substance causing the haemorrhaging was to remain unknown for many years. Over the coming years studies of the spoiled hay eventually led to the extraction of a compound which was later named dicoumarol. It was observed that this dicoumarol could not act as an anticoagulant on its own. It was only after it was metabolised byfungi that it exhibited anticoagulant properties. This explained why only spoiled hay caused the outbreak in the cattle. After further research, the synthesis of a more potent anticoagulant from dicoumarol, warfarin, was produced. Warfarin first commercial use was as a rat poison in 1948, followed by license for human use in 1953. This application brief illustrates the analysis of warfarin, Figure 1, using the Quasar AQ liquid chromatography phase.

  • Application Brief

    Analysis of Naproxen using Quasar SPP C18 Column

    Naproxen is a nonsteroidal anti-inflammatory drugs (NSAIDs) used to treat certain types of arthritis and other acute inflammatory conditions. It was first released to the prescription drug market in 1976 under the name Naprosyn. Then a few years later its counterpart salt, naproxen sodium, was released for prescription use and is used predominately in formulations today. The HPLC analysis of Naproxen on a porous silica C18 column is well documented in the literature. However, with the current trend in liquid chromatography being towards higher kinetic efficiency and shorter analysis time the subsequent development in column technology has realised that, without the need to change instrumentation. This application brief will illustrate the application of a superficially porous particles (SPP) column for the analysis of naproxen.

  • Application Note

    UHPLC-PDA Analysis of Curcuma Using a Quasar SPP Column

    Turmeric is the powdered dry rhizome of the plant Curcuma longa L., and is used as a coloring agent to give a yellow-orange color to food dishes.

    It has been found to be a rich source of phenolic compounds, namely, three curcuminoids: curcumin (C), demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC). Research to date suggests that turmeric, in addition to having an immunomodulatory role, is also of use in preventing oxidative stress that can lead to inflammation, cancer, and arthritis. This application note describes the sample preparation and analytical method for the chromatographic separation and quantitative monitoring of the three curcuminoids in commercially available turmeric spices and roots by UHPLC, using photodiode array (PDA) detection.

  • Application Brief

    Steroid Analysis using Quasar AQ HPLC Column

    Prednisolone, Prednisone and Cortisone are commonly used steroids to treat a range of inflammatory conditions and autoimmune disorders. These synthetic derivatives of hydrocortisone were developed and approved for medicinal use as early as the 1940’s. American chemists first identified Cortisone as having a therapeutic benefit in the treatment of rheumatoid arthritis and it was commercialized by Merck in 1948. The first commercially feasible synthesis of prednisone was carried out in 1955 in the laboratories of the Schering Corporation. A C18 HPLC column can be used for the analysis of these synthetic steroids, but they are not well retained, and resolution is incomplete. This application brief will look at the differences in chromatography between the Quasar C18 and AQ phase chemistries for the analysis prednisolone, prednisone and cortisone.

  • Application Note

    Compendium - Reliable Methods for Nutritional & Toxic Analysis of Breakfast Foods

    Breakfast is an important meal in providing essential nutrients to keep your energy levels up throughout the day. How do we ensure the food and beverages we consume are healthy, nutritious and safe? Following is a collection of application notes highlighting solutions that will help you identify micronutrients in milk, cereal, juice and fresh and dried fruits as well as toxic metals in tea, dairy products and apple juice to ensure safety of your breakfast foods.

  • Application Note

    Measurement of Counterfeit Pharmaceuticals Using the Spotlight Imaging System

    Counterfeiting of pharmaceuticals and the proliferation of substandard drugs constitute a serious health risk for the world population, including both industrialized and developing countries. It is estimated that five to eight percent of the world’s total pharmaceutical sales are counterfeit or of dubious quality. This application note discusses the identification of counterfeit drugs based on differences in the distribution of ingredients within the product.

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