Western Lightning ONE Pico, Chemiluminescent Substrate (500 mL)

Part of our premier line of premixed chemiluminescent HRP substrates, Western Lightning ONE Pico offers mid-picogram detection with no mixing required. Designed for sensitive and consistent detection of proteins, this single reagent format enables greater experimental consistency while remaining an economical choice for your everyday detection needs. Stored and ready-to-use at room temperature, the 12 month extended shelf life means you can buy and use Western Lightning ONE Pico with confidence. Unit size 2 x 250 mL.

Comparison of Western Lightning^™ ONE Versus Major Competitors

Performance and Signal Duration

• Western blotting detection of HDAC-1 in HeLa cell lysate
• Exposure time is 120 secs for each time point
• Images captured with ImageQuant^™ LAS 4000 from GE Healthcare

Performance	Signal Duration
Detection Level: Mid-picogram
Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane Primary antibody: Rabbit-anti Human HDAC-1 1:2000 Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:20000
Detection Level: Mid-femtogram
Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane Primary antibody: Rabbit-anti Human HDAC-1 1:5000 Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:75000
Detection Level: Low-femtogram
Two-fold dilution series of HeLa whole cell lysate from 2.5 μg to 0.039 μg of total protein on nitrocellulose membrane Primary antibody:  Rabbit-anti Human HDAC-1 1:10000 Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:200000