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Western Lightning ONE Femto Ultra, Chemiluminescent Substrate (250 mL)

When proteins are low and antibodies are precious, our extreme-level pre-mixed one-component chemiluminescent horseradish peroxidase (HRP) substrate with low antibody consumption is the right choice. Western Lightning ONE Femto Ultra can detect low-femtogram protein levels in western blots with superior signal intensity.

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Unit Size
List Price
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NEL150001EA
25 mL
124.00 USD
 
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NEL151001EA
125 mL
399.00 USD
 
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NEL152001EA
2 x 125 mL
689.00 USD
 
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Overview

Our most sensitive substrate in our premier line of premixed chemiluminescent HRP substrates, Western Lightning ONE Femto Ultra offers low-femtogram detection with excellent signal intensity. Western Lightning ONE Femto Ultra is designed with maximum sensitivity in a single reagent format. Stored and ready-to-use at room temperature, the 12 month extended shelf life means you can buy and use Western Lightning ONE Femto Ultra with confidence. Unit size 2 x 125 mL.

Comparison of Western Lightning ONE Versus Major Competitors

Performance and Signal Duration

  • Western blotting detection of HDAC-1 in HeLa cell lysate
  • Exposure time is 120 secs for each time point
  • Images captured with ImageQuant LAS 4000 from GE Healthcare
Performance Signal Duration
Detection Level: Mid-picogram
pico pico
  • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
  • Primary antibody: Rabbit-anti Human HDAC-1 1:2000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:20000
Detection Level: Mid-femtogram
femto femto
  • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
  • Primary antibody: Rabbit-anti Human HDAC-1 1:5000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:75000
Detection Level: Low-femtogram
femto ultra femto ultra
  • Two-fold dilution series of HeLa whole cell lysate from 2.5 μg to 0.039 μg of total protein on nitrocellulose membrane
  • Primary antibody:  Rabbit-anti Human HDAC-1 1:10000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:200000

Specifications

Detection Method Chemiluminescence
Product Brand Name Western Lightning ONE
Sample Type Chemiluminescent Substrate
Shipping Condition Ambient
Unit Size 2 x 125 mL
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Application Note

Western Blot Validation of CRISPR-Cas9-induced Functional Knockout using Synthetic sgRNA Molecules

Any well-designed CRISPR experiment must include a method to assess the accuracy and reliability of predicted gene editing results. This application note demonstrates how Horizon Discovery’s Edit-R predesigned synthetic sgRNAs result in high efficacy knockout o ...

PDF 1 MB

Flyer

The Power of Western Lightning ONE HRP Chemiluminescent Substrates

One-Reagent Substrates for Western Blotting

Get the consistency you demand from your western blot experiments with pre-mixed, one-reagent horseradish peroxidase (HRP) chemiluminescent substrates.

Western Lightning ONE, PerkinElmer’s premier line of premixed one-component chemilumines ...

PDF 1002 KB