Western Lightning ONE Femto Ultra, Chemiluminescent Substrate (25 mL)

Our most sensitive substrate in our premier line of premixed chemiluminescent HRP substrates, Western Lightning ONE Femto Ultra offers low-femtogram detection with excellent signal intensity. Western Lightning ONE Femto Ultra is designed with maximum sensitivity in a single reagent format. Stored and ready-to-use at room temperature, the 12 month extended shelf life means you can buy and use Western Lightning ONE Femto Ultra with confidence. Available in 25 mL size.

Comparison of Western Lightning^™ ONE Versus Major Competitors

Performance and Signal Duration

• Western blotting detection of HDAC-1 in HeLa cell lysate
• Exposure time is 120 secs for each time point
• Images captured with ImageQuant^™ LAS 4000 from GE Healthcare

Performance	Signal Duration
Detection Level: Mid-picogram
Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane Primary antibody: Rabbit-anti Human HDAC-1 1:2000 Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:20000
Detection Level: Mid-femtogram
Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane Primary antibody: Rabbit-anti Human HDAC-1 1:5000 Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:75000
Detection Level: Low-femtogram
Two-fold dilution series of HeLa whole cell lysate from 2.5 μg to 0.039 μg of total protein on nitrocellulose membrane Primary antibody:  Rabbit-anti Human HDAC-1 1:10000 Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:200000